Overview
- External ontology
- komodo.medium:210 (source link unavailable) — HALOANAEROBIUM medium
- Applications
-
- Microbial cultivation
- pH
- 9.0
- High-metal flag
- True
- Notes
- Source: KOMODO ModelSEED | ID: 210 | DSMZ Medium: 210 (mediadive.medium:210) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004390["haloanaerobium_medium"]
ing0_NaCl["NaCl"]
CultureMech_004390 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004390 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_004390 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Yeast_extract["Yeast extract"]
CultureMech_004390 --> ing3_Yeast_extract
ing4_Trypticase["Trypticase"]
CultureMech_004390 --> ing4_Trypticase
ing5_Glucose["Glucose"]
CultureMech_004390 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_NaOH["NaOH"]
CultureMech_004390 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_Na_thioglycolate["Na-thioglycolate"]
CultureMech_004390 --> ing7_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing7_Na_thioglycolate -.-> CHEBI_86481
ing8_Na_ascorbate["Na-ascorbate"]
CultureMech_004390 --> ing8_Na_ascorbate
CHEBI_113451["CHEBI:113451"]
ing8_Na_ascorbate -.-> CHEBI_113451
ing9_Biotin["Biotin"]
CultureMech_004390 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_004390 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_004390 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_004390 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Riboflavin["Riboflavin"]
CultureMech_004390 --> ing13_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing13_Riboflavin -.-> CHEBI_17015
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_004390 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_004390 --> ing15_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_004390 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_004390 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_004390 --> ing18__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_004390 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_004390 --> ing20_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004390 --> ing21_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004390 --> ing22_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
ing23_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004390 --> ing23_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing23_CaCl2_x_2_H2O -.-> CHEBI_86158
ing24_ZnCl2["ZnCl2"]
CultureMech_004390 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
ing25_CuCl2["CuCl2"]
CultureMech_004390 --> ing25_CuCl2
CHEBI_49553["CHEBI:49553"]
ing25_CuCl2 -.-> CHEBI_49553
ing26_H3BO3["H3BO3"]
CultureMech_004390 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004390 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004390 --> ing28_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_004390 --> ing29_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
122.495 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 121.495, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.2243 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.934579 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 | Yeast extract | 9.34579 G_PER_L | ||
| 5 | Trypticase | 9.34579 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 |
NaOH
HO.Na 39.997 g/mol
|
10 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 8 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
20 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 9 |
Na-ascorbate
C6H7O6.Na 198.106 g/mol
|
20 G_PER_L |
CHEBI:113451
sodium ascorbate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 22 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 23 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 24 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 26 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 30 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 210 |
| 2026-02-03T11:19:13.020616 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 210 |
| 2026-02-06T05:10:43.710110Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:27.421912Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:26.727616Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004390 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:41.868975+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOANAEROBIUM medium" to "haloanaerobium_medium" for consistent matching |
| 2026-06-05T07:40:21.665287+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.380276+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/4/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |