hutners_salts_medium_590

CultureMech:004392 bacterial DEFINED LIQUID
Original name: Hutner's salts (medium 590)

Overview

External ontology
komodo.medium:2110 (source link unavailable) — Hutner's salts (medium 590)
Applications
  • Microbial cultivation
pH
6.8
Notes
KOMODO 2110 records the Hutner's salts stock from DSMZ/MediaDive Medium 590. DSMZ Medium 590 defines Hutner's salts as 10.0 g/L nitrilotriacetic acid, 29.7 g/L MgSO4 x 7 H2O, 3.335 g/L CaCl2 x 2 H2O, 9.25 mg/L (NH4)6Mo7O24 x 4 H2O, 99.0 mg/L FeSO4 x 7 H2O, 50.0 ml/L Metals 44, and 950.0 ml/L distilled water adjusted to pH 6.8.

Composition

flowchart LR
    CultureMech_004392["hutners_salts_medium_590"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_004392 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_004392 --> ing1_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing1_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004392 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004392 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_004392 --> ing4__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing4__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004392 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
950.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 590 Hutner's salts lists 950.0 ml/L Distilled water.
2 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10.0 G_PER_L CHEBI:44557
nitrilotriacetic acid
 DSMZ Medium 590 Hutner's salts lists 10.0 g/L Nitrilotriacetic acid.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 DSMZ Medium 590 Hutner's salts lists 29.7 g/L MgSO4 x 7 H2O.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
 DSMZ Medium 590 Hutner's salts lists 3.335 g/L CaCl2 x 2 H2O.
5 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
9.25 MG_PER_L CHEBI:91249
ammonium molybdate
 DSMZ Medium 590 Hutner's salts lists 9.25 mg/L (NH4)6Mo7O24 x 4 H2O.
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
99.0 MG_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 DSMZ Medium 590 Hutner's salts lists 99.0 mg/L FeSO4 x 7 H2O.

Preparation

  1. Dissolve nitrilotriacetic acid.
  2. Adjust pH to 7.0 with about 7.3 g KOH.
  3. Dissolve MgSO4, CaCl2, ammonium molybdate, and FeSO4 separately.
  4. Combine the dissolved salts with 50.0 ml/L Metals 44.
  5. Adjust the final Hutner's salts stock to pH 6.8 with NaOH or H2SO4.

Solutions

"Metals 44"

Dose: 50.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Distilled water 1000.0 ML_PER_L DSMZ Medium 590 Metals 44 lists 1000.0 ml/L Distilled water.
Na-EDTA 250.0 MG_PER_L DSMZ Medium 590 Metals 44 lists 250.0 mg/L Na-EDTA.
ZnSO4 x 7 H2O 1095.0 MG_PER_L DSMZ Medium 590 Metals 44 lists 1095.0 mg/L ZnSO4 x 7 H2O.
FeSO4 x 7 H2O 500.0 MG_PER_L DSMZ Medium 590 Metals 44 lists 500.0 mg/L FeSO4 x 7 H2O.
MnSO4 x H2O 154.0 MG_PER_L DSMZ Medium 590 Metals 44 lists 154.0 mg/L MnSO4 x H2O.
CuSO4 x 5 H2O 39.2 MG_PER_L DSMZ Medium 590 Metals 44 lists 39.2 mg/L CuSO4 x 5 H2O.
Co(NO3)2 x 6 H2O 24.8 MG_PER_L DSMZ Medium 590 Metals 44 lists 24.8 mg/L Co(NO3)2 x 6 H2O.
Na2B4O7 x 10 H2O 17.7 MG_PER_L DSMZ Medium 590 Metals 44 lists 17.7 mg/L Na2B4O7 x 10 H2O.
DSMZ Medium 590 Hutner's salts adds 50.0 ml/L Metals 44.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 2110
2026-03-13T06:19:26.746561Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004392
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:41.892265+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hutner's salts (medium 590)" to "hutners_salts_medium_590" for consistent matching
2026-05-16T09:03:37.400314+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:08.850905+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_komodo_2110_hutners_salts_score15.py RESOLVED_KOMODO_2110_HUTNERS_SALTS_SCORE15 KOMODO 2110 records the Hutner's salts stock from DSMZ/MediaDive Medium 590. DSMZ Medium 590 defines Hutner's salts as 10.0 g/L nitrilotriacetic acid, 29.7 g/L MgSO4 x 7 H2O, 3.335 g/L CaCl2 x 2 H2O, 9.25 mg/L (NH4)6Mo7O24 x 4 H2O, 99.0 mg/L FeSO4 x 7 H2O, 50.0 ml/L Metals 44, and 950.0 ml/L distilled water adjusted to pH 6.8. Replaced the NaOH-only KOMODO note extraction with the complete DSMZ Hutner's salts stock, nested the Metals 44 stock, and grounded all quantified chemical components.