for_dsm_10643 (CultureMech:004422)

Overview

External ontology

komodo.medium:215c.1 (source link unavailable) — For DSM 10643

Applications

Microbial cultivation

Notes

KOMODO Medium 215c.1 records a DSM 10643 strain-specific DSMZ Medium 215c derivative with 37 g/L Brain heart infusion, 0.25 g/L Na2S x 9 H2O, 8.70 g/L glycerol, distilled water, 0.25 g/L Cysteine-HCl x H2O, and N2 gas.

Composition

flowchart LR
    CultureMech_004422["for_dsm_10643"]
    ing0_Brain_heart_infusion["Brain heart infusion"]
    CultureMech_004422 --> ing0_Brain_heart_infusion
    ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004422 --> ing1_Na2S_x_9_H2O
    ing2_glycerol["glycerol"]
    CultureMech_004422 --> ing2_glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_glycerol -.-> CHEBI_17754
    ing3_Distilled_water["Distilled water"]
    CultureMech_004422 --> ing3_Distilled_water
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_004422 --> ing4_L_Cysteine_HCl_x_H2O
    ing5_N2["N2"]
    CultureMech_004422 --> ing5_N2
    CHEBI_17997["CHEBI:17997"]
    ing5_N2 -.-> CHEBI_17997

Ingredients (6)

#	Ingredient	Concentration	Ontology	Notes
1	Brain heart infusion	37 G_PER_L	mediadive.compound:186 (source link unavailable) Brain heart infusion	KOMODO Medium 215c.1 lists Brain heart infusion at 37 g/L as an opaque rich-meat product.
2	Na2S x 9 H2O	0.25 G_PER_L	mediadive.compound:54 (source link unavailable) Na2S x 9 H2O	KOMODO Medium 215c.1 lists 0.25 g/L Na2S x 9 H2O.
3	glycerol C3H8O3 92.094 g/mol	8.70 G_PER_L	CHEBI:17754 glycerol	KOMODO Medium 215c.1 adds 8.70 g/L glycerol to the DSMZ Medium 215c base.
4	Distilled water	1000 ML_PER_L	mediadive.compound:4 (source link unavailable) Distilled water	KOMODO Medium 215c.1 brings the recipe to one liter with distilled water.
5	L-Cysteine HCl x H2O	0.25 G_PER_L	mediadive.compound:770 (source link unavailable) L-Cysteine HCl x H2O	KOMODO Medium 215c.1 lists 0.25 g/L L-Cysteine HCl x H2O.
6	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	KOMODO Medium 215c.1 lists N2 without a gram or molar amount.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 215c.1
2026-02-03T11:19:18.108477	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 215
2026-03-13T06:19:26.850577Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004422
2026-03-17T19:45:42.008601+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "For DSM 10643" to "for_dsm_10643" for consistent matching
2026-09-12T00:00:00-07:00	repair_komodo_215c_strict_anaerobes_score15.py	RESOLVED_DSMZ_KOMODO_215C_BHI_SCORE15	KOMODO Medium 215c.1 records a DSM 10643 strain-specific DSMZ Medium 215c derivative with 37 g/L Brain heart infusion, 0.25 g/L Na2S x 9 H2O, 8.70 g/L glycerol, distilled water, 0.25 g/L Cysteine-HCl x H2O, and N2 gas.