Overview
- External ontology
- komodo.medium:215c.2 (source link unavailable) — For DSM 15692
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- KOMODO Medium 215c.2 records a DSM 15692 strain-specific DSMZ Medium 215c derivative at pH 7.2 with NaHCO3, N2, and CO2 gas context.
Composition
flowchart LR
CultureMech_004423["for_dsm_15692"]
ing0_Brain_heart_infusion["Brain heart infusion"]
CultureMech_004423 --> ing0_Brain_heart_infusion
ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004423 --> ing1_Na2S_x_9_H2O
ing2_Distilled_water["Distilled water"]
CultureMech_004423 --> ing2_Distilled_water
ing3_CO2["CO2"]
CultureMech_004423 --> ing3_CO2
CHEBI_16526["CHEBI:16526"]
ing3_CO2 -.-> CHEBI_16526
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_004423 --> ing4_L_Cysteine_HCl_x_H2O
ing5_N2["N2"]
CultureMech_004423 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
ing6_NaHCO3["NaHCO3"]
CultureMech_004423 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brain heart infusion | 37 G_PER_L |
mediadive.compound:186 (source link unavailable)
Brain heart infusion
|
KOMODO Medium 215c.2 lists Brain heart infusion at 37 g/L as an opaque rich-meat product. |
| 2 | Na2S x 9 H2O | 0.25 G_PER_L |
mediadive.compound:54 (source link unavailable)
Na2S x 9 H2O
|
KOMODO Medium 215c.2 lists 0.25 g/L Na2S x 9 H2O. |
| 3 | Distilled water | 1000 ML_PER_L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
KOMODO Medium 215c.2 brings the recipe to one liter with distilled water. |
| 4 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
KOMODO Medium 215c.2 lists CO2 without a gram or molar amount. |
| 5 | L-Cysteine HCl x H2O | 0.25 G_PER_L |
mediadive.compound:770 (source link unavailable)
L-Cysteine HCl x H2O
|
KOMODO Medium 215c.2 lists 0.25 g/L L-Cysteine HCl x H2O. |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
KOMODO Medium 215c.2 lists N2 without a gram or molar amount. |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
variable VARIABLE |
CHEBI:32139
sodium hydrogencarbonate
|
KOMODO Medium 215c.2 records NaHCO3 in the pH 7.2 buffer context without a gram amount. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 215c.2 |
| 2026-02-03T11:19:12.993435 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 215 |
| 2026-03-13T03:09:27.368667Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaHCO3 |
| 2026-03-13T06:19:26.852925Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004423 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaHCO3 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:42.011460+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 15692" to "for_dsm_15692" for consistent matching |
| 2026-05-16T09:03:33.126743+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:02.166817+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_komodo_215c_strict_anaerobes_score15.py | RESOLVED_DSMZ_KOMODO_215C_BHI_SCORE15 | KOMODO Medium 215c.2 records a DSM 15692 strain-specific DSMZ Medium 215c derivative at pH 7.2 with NaHCO3, N2, and CO2 gas context. |