for_dsm_19851 (CultureMech:004424)

Overview

External ontology

komodo.medium:215c.3 (source link unavailable) — For DSM 19851

Applications

Microbial cultivation

Notes

KOMODO Medium 215c.3 records a DSM 19851 strain-specific DSMZ Medium 215c derivative with 36.63 g/L Brain heart infusion plus variable ethanol, Vitamin K1, and haemin.

Composition

flowchart LR
    CultureMech_004424["for_dsm_19851"]
    ing0_Brain_heart_infusion["Brain heart infusion"]
    CultureMech_004424 --> ing0_Brain_heart_infusion
    ing1_ethanol["ethanol"]
    CultureMech_004424 --> ing1_ethanol
    CHEBI_16236["CHEBI:16236"]
    ing1_ethanol -.-> CHEBI_16236
    ing2_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004424 --> ing2_Na2S_x_9_H2O
    ing3_Distilled_water["Distilled water"]
    CultureMech_004424 --> ing3_Distilled_water
    ing4_Vitamin_K1["Vitamin K1"]
    CultureMech_004424 --> ing4_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing4_Vitamin_K1 -.-> CHEBI_18067
    ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_004424 --> ing5_L_Cysteine_HCl_x_H2O
    ing6_haemin["haemin"]
    CultureMech_004424 --> ing6_haemin
    CHEBI_50385["CHEBI:50385"]
    ing6_haemin -.-> CHEBI_50385
    ing7_N2["N2"]
    CultureMech_004424 --> ing7_N2
    CHEBI_17997["CHEBI:17997"]
    ing7_N2 -.-> CHEBI_17997

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Brain heart infusion	36.63 G_PER_L	mediadive.compound:186 (source link unavailable) Brain heart infusion	KOMODO Medium 215c.3 lists Brain heart infusion at 36.63 g/L as an opaque rich-meat product.
2	ethanol C2H6O 46.069 g/mol	variable VARIABLE	CHEBI:16236 ethanol	KOMODO Medium 215c.3 lists ethanol without a gram or molar amount.
3	Na2S x 9 H2O	0.25 G_PER_L	mediadive.compound:54 (source link unavailable) Na2S x 9 H2O	KOMODO Medium 215c.3 lists 0.25 g/L Na2S x 9 H2O.
4	Distilled water	1000 ML_PER_L	mediadive.compound:4 (source link unavailable) Distilled water	KOMODO Medium 215c.3 brings the recipe to one liter with distilled water.
5	Vitamin K1 C31H46O2 450.707 g/mol	variable VARIABLE	CHEBI:18067 phylloquinone	KOMODO Medium 215c.3 lists Vitamin K1 without a gram or molar amount.
6	L-Cysteine HCl x H2O	0.25 G_PER_L	mediadive.compound:770 (source link unavailable) L-Cysteine HCl x H2O	KOMODO Medium 215c.3 lists 0.25 g/L L-Cysteine HCl x H2O.
7	haemin C34H32ClFeN4O4 651.952 g/mol	variable VARIABLE	CHEBI:50385 hemin	KOMODO Medium 215c.3 lists haemin without a gram or molar amount.
8	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	KOMODO Medium 215c.3 lists N2 without a gram or molar amount.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 215c.3
2026-02-03T11:19:07.365786	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 215
2026-03-13T06:19:26.855046Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004424
2026-03-17T19:45:42.013848+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "For DSM 19851" to "for_dsm_19851" for consistent matching
2026-09-12T00:00:00-07:00	repair_komodo_215c_strict_anaerobes_score15.py	RESOLVED_DSMZ_KOMODO_215C_BHI_SCORE15	KOMODO Medium 215c.3 records a DSM 19851 strain-specific DSMZ Medium 215c derivative with 36.63 g/L Brain heart infusion plus variable ethanol, Vitamin K1, and haemin.