Overview
- External ontology
- komodo.medium:215c (source link unavailable) — BHI medium FOR STRICT ANAEROBES
- Applications
-
- Microbial cultivation
- Notes
- KOMODO Medium 215c cites DSMZ Medium 215c and records the same 37 g/L Brain heart infusion, 0.25 g/L Na2S x 9 H2O, 0.25 g/L Cysteine-HCl x H2O, distilled water, and N2 gas atmosphere.
Composition
flowchart LR
CultureMech_004425["bhi_medium_for_strict_anaerobes"]
ing0_Brain_heart_infusion["Brain heart infusion"]
CultureMech_004425 --> ing0_Brain_heart_infusion
ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004425 --> ing1_Na2S_x_9_H2O
ing2_Distilled_water["Distilled water"]
CultureMech_004425 --> ing2_Distilled_water
ing3_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_004425 --> ing3_L_Cysteine_HCl_x_H2O
ing4_N2["N2"]
CultureMech_004425 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brain heart infusion | 37 G_PER_L |
mediadive.compound:186 (source link unavailable)
Brain heart infusion
|
KOMODO Medium 215c lists Brain heart infusion at 37 g/L as an opaque rich-meat product. |
| 2 | Na2S x 9 H2O | 0.25 G_PER_L |
mediadive.compound:54 (source link unavailable)
Na2S x 9 H2O
|
KOMODO Medium 215c lists 0.25 g/L Na2S x 9 H2O. |
| 3 | Distilled water | 1000 ML_PER_L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
KOMODO Medium 215c brings the recipe to one liter with distilled water. |
| 4 | L-Cysteine HCl x H2O | 0.25 G_PER_L |
mediadive.compound:770 (source link unavailable)
L-Cysteine HCl x H2O
|
KOMODO Medium 215c lists 0.25 g/L L-Cysteine HCl x H2O. |
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
KOMODO Medium 215c lists N2 without a gram or molar amount. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 215c |
| 2026-02-03T11:19:11.375464 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 215 |
| 2026-03-13T06:19:26.857017Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004425 |
| 2026-03-17T19:45:42.015782+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI medium FOR STRICT ANAEROBES" to "bhi_medium_for_strict_anaerobes" for consistent matching |
| 2026-09-12T00:00:00-07:00 | repair_komodo_215c_strict_anaerobes_score15.py | RESOLVED_DSMZ_KOMODO_215C_BHI_SCORE15 | KOMODO Medium 215c cites DSMZ Medium 215c and records the same 37 g/L Brain heart infusion, 0.25 g/L Na2S x 9 H2O, 0.25 g/L Cysteine-HCl x H2O, distilled water, and N2 gas atmosphere. |