mycobacterium_medium (CultureMech:004429)

Overview

External ontology

komodo.medium:219 (source link unavailable) — MYCOBACTERIUM medium

Applications

Microbial cultivation

pH

7.0

Notes

Source: KOMODO ModelSEED | ID: 219 | DSMZ Medium: 219 (mediadive.medium:219) | Aerobic: No

Composition

flowchart LR
    CultureMech_004429["mycobacterium_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_004429 --> ing0_Yeast_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_004429 --> ing1_Proteose_peptone_no__3
    ing2_Casein_peptone["Casein peptone"]
    CultureMech_004429 --> ing2_Casein_peptone
    ing3_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_004429 --> ing3_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing4_KH2PO4["KH2PO4"]
    CultureMech_004429 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Sodium_citrate["Sodium citrate"]
    CultureMech_004429 --> ing5_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing5_Sodium_citrate -.-> CHEBI_53258
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004429 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_Glycerol["Glycerol"]
    CultureMech_004429 --> ing7_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing7_Glycerol -.-> CHEBI_17754
    ing8_Tween["Tween"]
    CultureMech_004429 --> ing8_Tween
    ing9_Agar["Agar"]
    CultureMech_004429 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	Yeast extract	2 G_PER_L
2	Proteose peptone no. 3	2 G_PER_L
3	Casein peptone	2 G_PER_L
4	Na2HPO4 x 12 H2O HO4P.2Na 141.958 g/mol	2.5 G_PER_L	CHEBI:34683 disodium hydrogenphosphate
5	KH2PO4 H2O4P.K 136.084 g/mol	1 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
6	Sodium citrate C6H5O7.3Na 258.069 g/mol	1.5 G_PER_L	CHEBI:53258 sodium citrate
7	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.6 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
8	Glycerol C3H8O3 92.094 g/mol	50 G_PER_L	CHEBI:17754 glycerol
9	Tween	0.5 G_PER_L
10	Agar	20 G_PER_L	CHEBI:2509 agar

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 219
2026-02-03T11:19:03.784933	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 219
2026-03-13T06:19:26.868193Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004429
2026-03-17T06:30:07.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:42.027769+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MYCOBACTERIUM medium" to "mycobacterium_medium" for consistent matching
2026-06-05T07:40:21.757987+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.409775+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T21:02:15.484988+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 219 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.