Overview
- External ontology
- komodo.medium:220_13870 (source link unavailable) — MEDIUM 220 MODIFIED FOR DSM 13870
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- pH buffer: Na-sesquicarbonate | Source: KOMODO ModelSEED | ID: 220_13870 | DSMZ Medium: 220 (mediadive.medium:220) | Aerobic: No
Composition
flowchart LR
CultureMech_004443["medium_220_modified_for_dsm_13870"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_004443 --> ing0_Casein_peptone
ing1_Soy_peptone["Soy peptone"]
CultureMech_004443 --> ing1_Soy_peptone
ing2_NaCl["NaCl"]
CultureMech_004443 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar["Agar"]
CultureMech_004443 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_D____Glucose["D(+)-Glucose"]
CultureMech_004443 --> ing4_D____Glucose
CHEBI_17634["CHEBI:17634"]
ing4_D____Glucose -.-> CHEBI_17634
ing5_K2HPO4["K2HPO4"]
CultureMech_004443 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Na["Na"]
CultureMech_004443 --> ing6_Na
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 32.0 G_PER_L | [Merged 2 duplicates: 15.0, 17.0] | |
| 2 | Soy peptone | 8.0 G_PER_L | [Merged 2 duplicates: 5.0, 3.0] | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Collapsed 2 identical duplicates: 5.0, 5.0] |
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
D(+)-Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 | Na | variable VARIABLE | Extracted from recipe notes (pH buffer) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 220_13870 |
| 2026-02-03T11:19:07.500777 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 220 |
| 2026-03-13T03:09:12.103151Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:26.925266Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004443 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:42.090026+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 220 MODIFIED FOR DSM 13870" to "medium_220_modified_for_dsm_13870" for consistent matching |
| 2026-05-16T09:04:12.242780+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:16.605596+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.900274+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:38.304724+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |