medium_252_modified_for_dsm_41420

CultureMech:004641 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 252 MODIFIED FOR DSM 41420

Overview

External ontology
komodo.medium:252_41420 (source link unavailable) — MEDIUM 252 MODIFIED FOR DSM 41420
Applications
  • Microbial cultivation
pH
4.5
Notes
Source: KOMODO ModelSEED | ID: 252_41420 | DSMZ Medium: 252 (mediadive.medium:252) | Aerobic: No

Composition

flowchart LR
    CultureMech_004641["medium_252_modified_for_dsm_41420"]
    ing0_Starch["Starch"]
    CultureMech_004641 --> ing0_Starch
    CHEBI_28017["CHEBI:28017"]
    ing0_Starch -.-> CHEBI_28017
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_004641 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_004641 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004641 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_004641 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCO3["CaCO3"]
    CultureMech_004641 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_Agar["Agar"]
    CultureMech_004641 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Starch 10 G_PER_L CHEBI:28017
starch
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
6 CaCO3
CO3.Ca 100.086 g/mol
2 G_PER_L CHEBI:3311
calcium carbonate
7 Agar 20 G_PER_L CHEBI:2509
agar

Solutions

Trace salt solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 252_41420
2026-02-03T11:19:13.383719 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 252
2026-03-13T06:19:27.821185Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004641
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:43.104256+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 252 MODIFIED FOR DSM 41420" to "medium_252_modified_for_dsm_41420" for consistent matching
2026-06-05T07:41:17.549621+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:02.297526+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T07:04:22.005804+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).