Overview
- External ontology
- komodo.medium:253 (source link unavailable) — medium FOR ECTOTHIORHODOSPIRA
- Applications
-
- Microbial cultivation
- pH
- 8.5
- High-metal flag
- True
- Notes
- Source: KOMODO ModelSEED | ID: 253 | DSMZ Medium: 253 (mediadive.medium:253) | Aerobic: No
Composition
flowchart LR
CultureMech_004655["medium_for_ectothiorhodospira"]
ing0_KH2PO4["KH2PO4"]
CultureMech_004655 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004655 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_NH4Cl["NH4Cl"]
CultureMech_004655 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004655 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_NaCl["NaCl"]
CultureMech_004655 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2SO4["Na2SO4"]
CultureMech_004655 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_Na2CO3["Na2CO3"]
CultureMech_004655 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_Yeast_extract["Yeast extract"]
CultureMech_004655 --> ing7_Yeast_extract
ing8_Sodium_succinate["Sodium succinate"]
CultureMech_004655 --> ing8_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing8_Sodium_succinate -.-> CHEBI_63675
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004655 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004655 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004655 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004655 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnCl2["ZnCl2"]
CultureMech_004655 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_H3BO3["H3BO3"]
CultureMech_004655 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004655 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_004655 --> ing16_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing17_Nicotinamide["Nicotinamide"]
CultureMech_004655 --> ing17_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing17_Nicotinamide -.-> CHEBI_17154
ing18_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
CultureMech_004655 --> ing18_Pyridoxal_hydrochloride
CHEBI_131529["CHEBI:131529"]
ing18_Pyridoxal_hydrochloride -.-> CHEBI_131529
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_004655 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_004655 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_NaHCO3["NaHCO3"]
CultureMech_004655 --> ing21_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing21_NaHCO3 -.-> CHEBI_32139
ing22_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004655 --> ing22_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing22_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.49456 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.049456 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.791296 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.098912 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
178.042 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
19.7824 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5.93472 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 | Yeast extract | 0.49456 G_PER_L | ||
| 9 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.98912 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.25 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.07 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.01 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 18 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.35 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 19 |
Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
|
0.1 G_PER_L |
CHEBI:131529
pyridoxal hydrochloride
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
NaHCO3
CHO3.Na 84.006 g/mol
|
70 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 23 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
100 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Solutions
Trace element solution (A)
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.8 G_PER_L |
Vitamin solution (B)
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.3 G_PER_L | |
| p-Aminobenzoic acid | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 253 |
| 2026-02-03T11:18:54.615611 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 253 |
| 2026-03-13T06:19:27.901227Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004655 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.201542+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "medium FOR ECTOTHIORHODOSPIRA" to "medium_for_ectothiorhodospira" for consistent matching |
| 2026-06-05T07:40:21.903406+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.449917+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.286691+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution (A)' @ 1 ml/l; 3 -> 'Vitamin solution (B)' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |