Overview
- External ontology
- komodo.medium:269 (source link unavailable) — ACIDIPHILIUM medium
- Applications
-
- Microbial cultivation
- pH
- 3.0
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 269 | DSMZ Medium: 269 (mediadive.medium:269) | Aerobic: No
Composition
flowchart LR
CultureMech_004675["acidiphilium_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_004675 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KCl["KCl"]
CultureMech_004675 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_K2HPO4["K2HPO4"]
CultureMech_004675 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004675 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_004675 --> ing4_Yeast_extract
ing5_D_Glucose["D-Glucose"]
CultureMech_004675 --> ing5_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing5_D_Glucose -.-> CHEBI_17634
ing6_H2SO4["H2SO4"]
CultureMech_004675 --> ing6_H2SO4
CHEBI_26836["CHEBI:26836"]
ing6_H2SO4 -.-> CHEBI_26836
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Yeast extract | 0.3 G_PER_L | ||
| 6 |
D-Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 7 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 269 |
| 2026-02-03T11:18:58.923259 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 269 |
| 2026-03-13T03:08:49.139351Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: H2SO4 |
| 2026-03-13T06:19:27.997856Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004675 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.316802+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIPHILIUM medium" to "acidiphilium_medium" for consistent matching |
| 2026-05-16T09:03:01.948281+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:21.998984+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.481347+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |