Overview
- External ontology
- komodo.medium:280 (source link unavailable) — METHANOCOCCOIDES medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 280 | DSMZ Medium: 280 (mediadive.medium:280) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004733["methanococcoides_medium"]
ing0_KCl["KCl"]
CultureMech_004733 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004733 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004733 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NH4Cl["NH4Cl"]
CultureMech_004733 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004733 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_K2HPO4["K2HPO4"]
CultureMech_004733 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_NaCl["NaCl"]
CultureMech_004733 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_004733 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_Na_acetate["Na-acetate"]
CultureMech_004733 --> ing8_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Na_acetate -.-> CHEBI_32954
ing9_Yeast_extract["Yeast extract"]
CultureMech_004733 --> ing9_Yeast_extract
ing10_Trypticase_peptone["Trypticase peptone"]
CultureMech_004733 --> ing10_Trypticase_peptone
ing11_Sodium_resazurin["Sodium resazurin"]
CultureMech_004733 --> ing11_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Sodium_resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_004733 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Trimethylamine_HCl["Trimethylamine-HCl"]
CultureMech_004733 --> ing13_Trimethylamine_HCl
CHEBI_64700["CHEBI:64700"]
ing13_Trimethylamine_HCl -.-> CHEBI_64700
ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_004733 --> ing14_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004733 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_004733 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_004733 --> ing17_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing17_MnSO4_x_H2O -.-> CHEBI_86364
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004733 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_004733 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004733 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_004733 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_004733 --> ing22_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing23_H3BO3["H3BO3"]
CultureMech_004733 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004733 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004733 --> ing25_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_004733 --> ing26_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_004733 --> ing27_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing28_Biotin["Biotin"]
CultureMech_004733 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_004733 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_004733 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.335637 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.94867 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.40573 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.40573, 3.0] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.246792 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.238203 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.138203, 0.1] |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138203 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
18.769 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 17.769, 1.0] |
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00197433 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.987167 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 10 | Yeast extract | 1.97433 G_PER_L | ||
| 11 | Trypticase peptone | 1.97433 G_PER_L | ||
| 12 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000493583 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.93583 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Trimethylamine-HCl
C3H9N.HCl 95.573 g/mol
|
2.9615 G_PER_L |
CHEBI:64700
trimethylamine hydrochloride
|
|
| 15 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.493583 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.493583 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 18 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 26 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 27 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 28 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 35 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 36 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 37 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 38 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 280 |
| 2026-02-03T11:19:05.522681 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 280 |
| 2026-02-06T05:10:30.558486Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:06.353172Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:28.338087Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004733 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.725933+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOCOCCOIDES medium" to "methanococcoides_medium" for consistent matching |
| 2026-05-20T06:00:50.966782+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.0-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:22.084938+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.500355+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |