methanosarcina_acetivorans_medium (CultureMech:004832)

Overview

External ontology

komodo.medium:304 (source link unavailable) — METHANOSARCINA ACETIVORANS medium

Applications

Microbial cultivation

pH

7.0

Notes

Archived DSMZ Medium 304 defines METHANOSARCINA ACETIVORANS MEDIUM with base salts, yeast extract, 10 mL DSMZ Medium 141 trace element solution, resazurin, trimethylamine hydrochloride or methanol, cysteine hydrochloride hydrate, sodium sulfide nonahydrate, 80:20 N2/CO2, and pH 7.0; the live KOMODO Medium 304 table selects the trimethylamine hydrochloride branch.

Composition

flowchart LR
    CultureMech_004832["methanosarcina_acetivorans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_004832 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004832 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Na2CO3["Na2CO3"]
    CultureMech_004832 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_004832 --> ing3_Yeast_extract
    ing4_NH4Cl["NH4Cl"]
    CultureMech_004832 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KCl["KCl"]
    CultureMech_004832 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004832 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2HPO4["Na2HPO4"]
    CultureMech_004832 --> ing7_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing7_Na2HPO4 -.-> CHEBI_34683
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_004832 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_004832 --> ing9_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing9_MnSO4_x_H2O -.-> CHEBI_86364
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004832 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_004832 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004832 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_004832 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_004832 --> ing14_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing14_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing15_H3BO3["H3BO3"]
    CultureMech_004832 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004832 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004832 --> ing17_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_004832 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Resazurin["Resazurin"]
    CultureMech_004832 --> ing19_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing19_Resazurin -.-> CHEBI_8806
    ing20_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_004832 --> ing20_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing20_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing21_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004832 --> ing21_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing21_Na2S_x_9_H2O -.-> CHEBI_76209
    ing22_Trimethylamine_HCl["Trimethylamine-HCl"]
    CultureMech_004832 --> ing22_Trimethylamine_HCl
    CHEBI_64700["CHEBI:64700"]
    ing22_Trimethylamine_HCl -.-> CHEBI_64700
    ing23_HCl["HCl"]
    CultureMech_004832 --> ing23_HCl
    CHEBI_17883["CHEBI:17883"]
    ing23_HCl -.-> CHEBI_17883
    ing24_KOH["KOH"]
    CultureMech_004832 --> ing24_KOH
    CHEBI_32035["CHEBI:32035"]
    ing24_KOH -.-> CHEBI_32035
    ing25_N2["N2"]
    CultureMech_004832 --> ing25_N2
    CHEBI_17997["CHEBI:17997"]
    ing25_N2 -.-> CHEBI_17997
    ing26_CO2["CO2"]
    CultureMech_004832 --> ing26_CO2
    CHEBI_16526["CHEBI:16526"]
    ing26_CO2 -.-> CHEBI_16526
    ing27_Distilled_water["Distilled water"]
    CultureMech_004832 --> ing27_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing27_Distilled_water -.-> CHEBI_15377

Ingredients (28)

#	Ingredient	Concentration	Ontology	Notes
1	NaCl Cl.Na 58.443 g/mol	23.178218 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 304 lists 23.40 g NaCl and adds 10 mL of the Archived DSMZ Medium 141 trace element solution containing 1.00 g/L NaCl to 1000 mL water, yielding 23.178218 g/L in the 1010 mL final formulation.
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	9.386139 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 304 lists 9.45 g MgSO4 x 7 H2O and adds 10 mL of the Archived DSMZ Medium 141 trace element solution containing 3.00 g/L MgSO4 x 7 H2O to 1000 mL water, yielding 9.386139 g/L in the 1010 mL final formulation.
3	Na2CO3 CO3.2Na 105.988 g/mol	4.950495 G_PER_L	CHEBI:29377 sodium carbonate	Archived DSMZ Medium 304 lists 5.00 g Na2CO3 and 10 mL of trace element solution in 1000 mL water, yielding 4.950495 g/L in the 1010 mL final formulation.
4	Yeast extract	0.990099 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 304 lists 1.00 g Yeast extract and 10 mL of trace element solution in 1000 mL water, yielding 0.990099 g/L in the 1010 mL final formulation.
5	NH4Cl Cl.H4N 53.492 g/mol	0.990099 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 304 lists 1.00 g NH4Cl and 10 mL of trace element solution in 1000 mL water, yielding 0.990099 g/L in the 1010 mL final formulation.
6	KCl Cl.K 74.551 g/mol	0.792079 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 304 lists 0.80 g KCl and 10 mL of trace element solution in 1000 mL water, yielding 0.792079 g/L in the 1010 mL final formulation.
7	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.139604 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 304 lists 0.14 g CaCl2 x 2 H2O and adds 10 mL of the Archived DSMZ Medium 141 trace element solution containing 0.10 g/L CaCl2 x 2 H2O to 1000 mL water, yielding 0.139604 g/L in the 1010 mL final formulation.
8	Na2HPO4 HO4P.2Na 141.958 g/mol	0.594059 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Archived DSMZ Medium 304 lists 0.60 g Na2HPO4 and 10 mL of trace element solution in 1000 mL water, yielding 0.594059 g/L in the 1010 mL final formulation.
9	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.014851 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 1.50 g/L Nitrilotriacetic acid, yielding 0.014851 g/L in the 1010 mL final formulation.
10	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.004950 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.50 g/L MnSO4 x H2O, yielding 0.004950 g/L in the 1010 mL final formulation.
11	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.000990 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.10 g/L FeSO4 x 7 H2O, yielding 0.000990 g/L in the 1010 mL final formulation.
12	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.001782 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.18 g/L CoSO4 x 7 H2O, yielding 0.001782 g/L in the 1010 mL final formulation.
13	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.001782 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.18 g/L ZnSO4 x 7 H2O, yielding 0.001782 g/L in the 1010 mL final formulation.
14	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.0000990 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.01 g/L CuSO4 x 5 H2O, yielding 0.0000990 g/L in the 1010 mL final formulation.
15	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000198 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.02 g/L KAl(SO4)2 x 12 H2O, yielding 0.000198 g/L in the 1010 mL final formulation.
16	H3BO3 H3BO3 61.833 g/mol	0.0000990 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.01 g/L H3BO3, yielding 0.0000990 g/L in the 1010 mL final formulation.
17	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000990 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.01 g/L Na2MoO4 x 2 H2O, yielding 0.0000990 g/L in the 1010 mL final formulation.
18	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000297 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.03 g/L NiCl2 x 6 H2O, yielding 0.000297 g/L in the 1010 mL final formulation.
19	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.000002970 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 304 adds 10 mL of the Archived DSMZ Medium 141 trace element solution to 1000 mL water; the stock contains 0.30 mg/L Na2SeO3 x 5 H2O, yielding 0.000002970 g/L in the 1010 mL final formulation.
20	Resazurin C12H7NO4 229.191 g/mol	0.000990 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 304 lists 1.00 mg Resazurin and 10 mL of trace element solution in 1000 mL water, yielding 0.000990 g/L in the 1010 mL final formulation.
21	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.247525 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 304 lists 0.25 g Cysteine-HCl x H2O and 10 mL of trace element solution in 1000 mL water, yielding 0.247525 g/L in the 1010 mL final formulation.
22	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.247525 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 304 lists 0.25 g Na2S x 9 H2O and 10 mL of trace element solution in 1000 mL water, yielding 0.247525 g/L in the 1010 mL final formulation.
23	Trimethylamine-HCl C3H9N.HCl 95.573 g/mol	2.970297 G_PER_L	CHEBI:64700 trimethylamine hydrochloride	Archived DSMZ Medium 304 lists Trimethylamine-HCl as an alternative substrate for the medium and live KOMODO selects this branch, yielding 2.970297 g/L in the 1010 mL final formulation.
24	HCl HCl 36.461 g/mol	variable VARIABLE	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 304 adjusts the final medium to pH 7.0 with hydrochloric acid.
25	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	Archived DSMZ Medium 141 trace element solution adjusts the trace element solution with KOH.
26	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 304 prepares the medium under 80% N2 and 20% CO2.
27	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 304 prepares the medium under 80% N2 and 20% CO2.
28	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 304 lists 1000 mL distilled water before the trace addition.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 304
2026-03-13T06:19:28.871195Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004832
2026-03-17T19:45:44.353209+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOSARCINA ACETIVORANS medium" to "methanosarcina_acetivorans_medium" for consistent matching
2026-08-06T08:58:32.358216+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_304_score30.py	RESOLVED_KOMODO_304_SCORE30	Archived DSMZ Medium 304 defines METHANOSARCINA ACETIVORANS MEDIUM with base salts, yeast extract, 10 mL DSMZ Medium 141 trace element solution, resazurin, trimethylamine hydrochloride or methanol, cysteine hydrochloride hydrate, sodium sulfide nonahydrate, 80:20 N2/CO2, and pH 7.0; the live KOMODO Medium 304 table selects the trimethylamine hydrochloride branch.