for_dsm_2805 (CultureMech:004855)

Overview

External ontology

komodo.medium:307.1 (source link unavailable) — For DSM 2805

Applications

Microbial cultivation

pH

7.2

Notes

BacDive curates DSM 2805 as SYNTHROPHOMONAS MEDIUM, DSMZ Medium 212 with strain-specific modifications, and lists the flattened final DSM 2805 composition with Na-propionate and D-Glucose. All 32 rates are the exact BacDive strain-specific provider values, not independently recomputed final concentrations from DSMZ instructions. Distilled water is listed without a number. Existing growth assertions are preserved, not newly verified by these quantity claims. The DOI redirect was located but its versioned PDF was not inspected; evidence cites the captured strain-page PURL only. Archive the previous formulation nodes in append-only history. The inspected complete source defines this reconstruction, including removal of false LB expansion, placeholders and flattened stock quantities. Prior evidence content is preserved; same-reference explanations may be extended, never replaced.

Composition

flowchart LR
    CultureMech_004855["for_dsm_2805"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_004855 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_Na_propionate["Na-propionate"]
    CultureMech_004855 --> ing1_Na_propionate
    CHEBI_132106["CHEBI:132106"]
    ing1_Na_propionate -.-> CHEBI_132106
    ing2_Na2CO3["Na2CO3"]
    CultureMech_004855 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_D_Glucose["D-Glucose"]
    CultureMech_004855 --> ing3_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_D_Glucose -.-> CHEBI_17634
    ing4_Trypticase_peptone["Trypticase peptone"]
    CultureMech_004855 --> ing4_Trypticase_peptone
    ing5_KH2PO4["KH2PO4"]
    CultureMech_004855 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NaCl["NaCl"]
    CultureMech_004855 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_NH4Cl["NH4Cl"]
    CultureMech_004855 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_004855 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004855 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_004855 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004855 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_HCl["HCl"]
    CultureMech_004855 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_004855 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_Sodium_resazurin["Sodium resazurin"]
    CultureMech_004855 --> ing14_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Sodium_resazurin -.-> CHEBI_8806
    ing15_NaOH["NaOH"]
    CultureMech_004855 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_004855 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_004855 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004855 --> ing18_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing18_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004855 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_004855 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_004855 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004855 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_004855 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_ZnCl2["ZnCl2"]
    CultureMech_004855 --> ing24_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing24_ZnCl2 -.-> CHEBI_49976
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004855 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004855 --> ing26_NiCl2_x_6_H2O
    ing27_D_____biotin["D-(+)-biotin"]
    CultureMech_004855 --> ing27_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_D_____biotin -.-> CHEBI_15956
    ing28_H3BO3["H3BO3"]
    CultureMech_004855 --> ing28_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing28_H3BO3 -.-> CHEBI_33118
    ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_004855 --> ing29_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
    CultureMech_004855 --> more["...3 more"]

Ingredients (33)

#	Ingredient	Concentration	Ontology	Notes
1	Na2SO4 2Na.O4S 142.043 g/mol	2.79163 G_PER_L	CHEBI:32149 sodium sulfate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
2	Na-propionate C3H5O2.Na 96.061 g/mol	1.49551 G_PER_L	CHEBI:132106 sodium propionate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
3	Na2CO3 CO3.2Na 105.988 g/mol	1.49551 G_PER_L	CHEBI:29377 sodium carbonate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
4	D-Glucose C6H12O6 180.156 g/mol	0.997009 G_PER_L	CHEBI:17634 D-glucose	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
5	Trypticase peptone	0.997008 G_PER_L		BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
6	KH2PO4 H2O4P.K 136.084 g/mol	0.498505 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
7	NaCl Cl.Na 58.443 g/mol	0.398803 G_PER_L	CHEBI:26710 sodium chloride	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
8	NH4Cl Cl.H4N 53.492 g/mol	0.398803 G_PER_L	CHEBI:31206 ammonium chloride	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
9	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.329013 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
10	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.299103 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
11	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.299103 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
12	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.0498505 G_PER_L	CHEBI:86158 calcium chloride dihydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
13	HCl HCl 36.461 g/mol	0.00249252 G_PER_L	CHEBI:17883 hydrogen chloride	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
14	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.00149551 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
15	Sodium resazurin C12H7NO4 229.191 g/mol	0.000498505 G_PER_L	CHEBI:8806 Resazurin	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
16	NaOH HO.Na 39.997 g/mol	0.000498504 G_PER_L	CHEBI:32145 sodium hydroxide	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
17	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	0.000299103 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
18	Nicotinic acid C6H5NO2 123.111 g/mol	0.000199402 G_PER_L	CHEBI:15940 nicotinic acid	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
19	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000199402 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
20	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000189432 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
21	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	9.97009e-05 G_PER_L	CHEBI:31345 Calcium pantothenate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
22	Vitamin B12	9.97009e-05 G_PER_L	CHEBI:176843 vitamin B12	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
23	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	9.97009e-05 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
24	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	7.97607e-05 G_PER_L	CHEBI:30753 4-aminobenzoic acid	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
25	ZnCl2 2Cl.Zn 136.296 g/mol	6.97906e-05 G_PER_L	CHEBI:49976 zinc dichloride	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
26	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3.58923e-05 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
27	NiCl2 x 6 H2O	2.39282e-05 G_PER_L		BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications) Withdraw only the generic CHEBI:34887 assertions. Keep the source-explicit hexahydrate label and quantity, and the verified upstream compound:40 accession where already present. Do not substitute the MIM REJECTED candidate, infer an anhydrous equivalent, or change mass rates using molecular weight. Curator identity check: packaged MIM index SHA256=a36cd4683feb89e2fc2a1721dc15008d1e10c12044e89a36c27b6621a8aaf262 reports the alternate candidate as REJECTED; no replacement chemical identifier is asserted.
28	D-(+)-biotin C10H16N2O3S 244.316 g/mol	1.99402e-05 G_PER_L	CHEBI:15956 biotin	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
29	H3BO3 H3BO3 61.833 g/mol	5.98205e-06 G_PER_L	CHEBI:33118 boric acid	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
30	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	3.98804e-06 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
31	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	2.99103e-06 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
32	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	1.99402e-06 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	BacDive lists this final concentration for the DSM 2805 strain-specific DSMZ Medium 212 formulation. Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)
33	Distilled water			Preserve the explicitly strain-specific BacDive rate, not an independent calculation from the rounded DSMZ variant instructions. No new growth assertion. Additional exact source excerpt: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications)

Preparation

Prepare the anaerobic DSMZ Medium 212 formulation with the DSM 2805 final carbon sources, Na-propionate and D-Glucose, under an 80% N2 and 20% CO2 atmosphere.
Dispense the basal solution under 80% N2 and 20% CO2 into anoxic Hungate-type tubes and autoclave; autoclave carbonate, carbon-source, cysteine, and sulfide solutions separately under anoxic gas.
Prepare the seven-vitamins solution under 100% N2 and sterilize it by filtration.
Add the sterile supplement solutions to sterile anoxic basal medium after autoclaving.
Adjust the complete medium to pH 7.2 if necessary.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Syntrophobacter wolinii DSM 2805
Term
Id
NCBITaxon:29527
Label
Syntrophobacter wolinii
Strain
DB; DSM 2805
Evidence
- Reference
  https://bacdive.dsmz.de/strain/16667
  Supports
  SUPPORT
  Explanation
  BacDive lists Syntrophobacter wolinii DSM 2805 as cultivated on SYNTHROPHOMONAS MEDIUM, DSMZ Medium 212 with strain-specific modifications, at 35 C under anaerobic conditions.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 307.1
2026-03-13T06:19:28.907758Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004855
2026-03-17T19:45:44.397976+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "For DSM 2805" to "for_dsm_2805" for consistent matching
2026-08-06T08:58:32.435088+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-06T00:00:00-07:00	repair_bacterial_strain_specific_score45.py	RESOLVED_STRAIN_SPECIFIC_SCORE45_GRAPH	BacDive curates DSM 2805 as SYNTHROPHOMONAS MEDIUM, DSMZ Medium 212 with strain-specific modifications, and lists the flattened final DSM 2805 composition with Na-propionate and D-Glucose.
2026-10-09T11:24:57.076143+00:00	codex	RESTORED_SYNTHROPHOMONAS_SOURCE_FORMULATIONS_WITH_EVIDENCE	Restore source-specific medium212 formulations, stock-stage inputs and literal quantity evidence. Preserve old evidence content and history. Same-reference explanations are append-only; no final-volume, density, percent-basis or growth assertion is inferred.