scy_medium

CultureMech:004977 bacterial COMPLEX LIQUID
Original name: SCY - medium

Overview

External ontology
komodo.medium:312 (source link unavailable) — SCY - medium
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: KOMODO ModelSEED | ID: 312 | DSMZ Medium: 312 (mediadive.medium:312) | Aerobic: No Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_004977["scy_medium"]
    ing0_Sucrose["Sucrose"]
    CultureMech_004977 --> ing0_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing0_Sucrose -.-> CHEBI_17992
    ing1_Casitone["Casitone"]
    CultureMech_004977 --> ing1_Casitone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_004977 --> ing2_Yeast_extract
    ing3_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_004977 --> ing3_Pancreatic_digest_of_casein
    ing4_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_004977 --> ing4_Peptic_digest_of_soybean_meal
    ing5_Glucose["Glucose"]
    CultureMech_004977 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_004977 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_004977 --> ing7_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing7_Dipotassium_phosphate -.-> CHEBI_131527
    ing8_Agar["Agar"]
    CultureMech_004977 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004977 --> ing9_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing9_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_004977 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Sucrose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17992
sucrose
2 Casitone 0.75 G_PER_L
3 Yeast extract 0.25 G_PER_L
4 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
8 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
9 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
10 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.0004 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
11 Vitamin B12 1e-05 G_PER_L CHEBI:176843
vitamin B12

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 312
2026-02-03T11:19:08.491581 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 312
2026-03-13T06:19:29.294027Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004977
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:44.848475+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SCY - medium" to "scy_medium" for consistent matching
2026-06-05T07:40:22.362046+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.