Overview
- External ontology
- komodo.medium:3136 (source link unavailable) — Fastidious Anaerobe Agar
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- KOMODO Medium 3136 resolves to MediaDive/DSMZ Medium 1203: 45.7 g Acumedia Fastidious Anaerobe Agar per liter with 5-10% sterile defibrinated horse blood.
Composition
flowchart LR
CultureMech_004984["fastidious_anaerobe_agar"]
ing0_Fastidious_Anaerobe_Agar__Acumedia_["Fastidious Anaerobe Agar (Acumedia)"]
CultureMech_004984 --> ing0_Fastidious_Anaerobe_Agar__Acumedia_
ing1_Deionized_water["Deionized water"]
CultureMech_004984 --> ing1_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing1_Deionized_water -.-> CHEBI_15377
ing2_Sterile_defibrinated_horse_blood["Sterile defibrinated horse blood"]
CultureMech_004984 --> ing2_Sterile_defibrinated_horse_blood
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Fastidious Anaerobe Agar (Acumedia) | 45.7 G_PER_L | DSMZ Medium 1203 lists this commercial Acumedia agar base as supplied. | |
| 2 |
Deionized water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
DSMZ Medium 1203 brings the agar base to 1000 ml. |
| 3 | Sterile defibrinated horse blood | 5-10 PERCENT_V_V |
UBERON:0000178
blood
|
DSMZ Medium 1203 instructs adding 5-10% sterile defibrinated horse blood after autoclaving and cooling to 47 C. |
Preparation
- Allow the agar powder to soak for 10 min, then swirl to mix.
- Autoclave the mixed medium at 121 C for 15 min.
- Cool the sterilized medium to 47 C.
- Aseptically add 5-10% sterile defibrinated horse blood, mix well, and pour plates.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 3136 |
| 2026-03-13T06:19:29.304994Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004984 |
| 2026-03-17T19:45:44.862683+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fastidious Anaerobe Agar" to "fastidious_anaerobe_agar" for consistent matching |
| 2026-05-20T06:00:51.128144+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.0-7.4' -> {'min': 7.0, 'max': 7.4} |
| 2026-08-06T08:58:32.377425+00:00 | curate_flag_empty_composition.py | FLAGGED_INCOMPLETE_COMPOSITION | Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175). |
| 2026-08-07T05:25:54.947087+00:00 | mediadive-composition-backfill-v1.0 | BACKFILLED_COMPOSITION | Backfilled 3 components from MediaDive medium 1203 (/rest/medium-composition/1203). The record carried only the KOMODO id 3136; MediaDive 1203 is the same medium — identical name and identical pH range 7.0-7.4. Cleared incomplete_composition (#239). |
| 2026-09-10T00:00:00-07:00 | repair_dsmz_1203_fastidious_score20.py | RESOLVED_DSMZ_1203_FASTIDIOUS_SCORE20 | KOMODO Medium 3136 resolves to MediaDive/DSMZ Medium 1203: 45.7 g Acumedia Fastidious Anaerobe Agar per liter with 5-10% sterile defibrinated horse blood. |