acetobacterium_carbinolicum_medium (CultureMech:004988)

Overview

External ontology

komodo.medium:313 (source link unavailable) — ACETOBACTERIUM CARBINOLICUM medium

Applications

Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Archived DSMZ Medium 313 defines ACETOBACTERIUM CARBINOLICUM MEDIUM as DSMZ Medium 124 with 20 mM ethanol as the substrate; this record expands the archived DSMZ Medium 124 base, Solution B, trace stock, and vitamin stock into final per-liter components.

Composition

flowchart LR
    CultureMech_004988["acetobacterium_carbinolicum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_004988 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_004988 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_004988 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004988 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_004988 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_004988 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2SO4["Na2SO4"]
    CultureMech_004988 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_004988 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_ZnCl2["ZnCl2"]
    CultureMech_004988 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004988 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_004988 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004988 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004988 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004988 --> ing13_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004988 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_HCl["HCl"]
    CultureMech_004988 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_Na_acetate["Na-acetate"]
    CultureMech_004988 --> ing16_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing16_Na_acetate -.-> CHEBI_32954
    ing17_Na_butyrate["Na-butyrate"]
    CultureMech_004988 --> ing17_Na_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing17_Na_butyrate -.-> CHEBI_64103
    ing18_Yeast_extract["Yeast extract"]
    CultureMech_004988 --> ing18_Yeast_extract
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_004988 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_D____Biotin["D(+)-Biotin"]
    CultureMech_004988 --> ing20_D____Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_D____Biotin -.-> CHEBI_15956
    ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004988 --> ing21_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing22_Resazurin["Resazurin"]
    CultureMech_004988 --> ing22_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing22_Resazurin -.-> CHEBI_8806
    ing23_NaHCO3["NaHCO3"]
    CultureMech_004988 --> ing23_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing23_NaHCO3 -.-> CHEBI_32139
    ing24_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004988 --> ing24_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing24_Na2S_x_9_H2O -.-> CHEBI_76209
    ing25_Ethanol["Ethanol"]
    CultureMech_004988 --> ing25_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing25_Ethanol -.-> CHEBI_16236
    ing26_Distilled_water["Distilled water"]
    CultureMech_004988 --> ing26_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing26_Distilled_water -.-> CHEBI_15377

Ingredients (27)

#	Ingredient	Concentration	Ontology	Notes
1	NaCl Cl.Na 58.443 g/mol	1.156126 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 124 contributes 1.17 g NaCl in a 1012 mL final formulation, yielding 1.156126 g/L.
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.395257 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 124 contributes 0.40 g MgCl2 x 6 H2O in a 1012 mL final formulation, yielding 0.395257 g/L.
3	KCl Cl.K 74.551 g/mol	0.296443 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 124 contributes 0.30 g KCl in a 1012 mL final formulation, yielding 0.296443 g/L.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.148221 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 124 contributes 0.15 g CaCl2 x 2 H2O in a 1012 mL final formulation, yielding 0.148221 g/L.
5	NH4Cl Cl.H4N 53.492 g/mol	0.266798 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 124 contributes 0.27 g NH4Cl in a 1012 mL final formulation, yielding 0.266798 g/L.
6	KH2PO4 H2O4P.K 136.084 g/mol	0.197628 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 124 contributes 0.20 g KH2PO4 in a 1012 mL final formulation, yielding 0.197628 g/L.
7	Na2SO4 2Na.O4S 142.043 g/mol	2.806324 G_PER_L	CHEBI:32149 sodium sulfate	Archived DSMZ Medium 124 contributes 2.84 g Na2SO4 in a 1012 mL final formulation, yielding 2.806324 g/L.
8	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001482 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001482 g/L.
9	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000672 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.068 g/L ZnCl2, yielding 0.0000672 g/L.
10	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000988 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000988 g/L.
11	H3BO3 H3BO3 61.833 g/mol	0.0000613 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.062 g/L H3BO3, yielding 0.0000613 g/L.
12	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000119 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.120 g/L CoCl2 x 6 H2O, yielding 0.000119 g/L.
13	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.0000168 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.017 g/L CuCl2 x 2 H2O, yielding 0.0000168 g/L.
14	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000237 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000237 g/L.
15	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000237 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 trace element solution per 1012 mL final formulation; the trace stock contains 0.024 g/L Na2MoO4 x 2 H2O, yielding 0.0000237 g/L.
16	HCl HCl 36.461 g/mol	0.049407 MILLIMOLAR	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 124 adds 1 mL of 0.05 M HCl trace stock per 1012 mL final formulation, yielding 0.049407 mM HCl.
17	Na-acetate C2H3O2.Na 82.034 g/mol	1.383399 G_PER_L	CHEBI:32954 sodium acetate	Archived DSMZ Medium 124 contributes 1.40 g Na-acetate in a 1012 mL final formulation, yielding 1.383399 g/L.
18	Na-butyrate C4H7O2.Na 110.088 g/mol	1.383399 G_PER_L	CHEBI:64103 sodium butyrate	Archived DSMZ Medium 124 contributes 1.40 g Na-butyrate in a 1012 mL final formulation, yielding 1.383399 g/L.
19	Yeast extract	0.988142 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 124 contributes 1.00 g Yeast extract in a 1012 mL final formulation, yielding 0.988142 g/L.
20	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.0000395 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 vitamin solution per 1012 mL final formulation; the vitamin stock contains 0.040 g/L p-Aminobenzoic acid, yielding 0.0000395 g/L.
21	D(+)-Biotin C10H16N2O3S 244.316 g/mol	0.00000988 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 vitamin solution per 1012 mL final formulation; the vitamin stock contains 0.010 g/L D(+)-Biotin, yielding 0.00000988 g/L.
22	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.0000988 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 124 adds 1 mL of the Archived DSMZ Medium 124 vitamin solution per 1012 mL final formulation; the vitamin stock contains 0.100 g/L Thiamine-HCl x 2 H2O, yielding 0.0000988 g/L.
23	Resazurin C12H7NO4 229.191 g/mol	0.000494 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 124 contributes 0.50 mg Resazurin in a 1012 mL final formulation, yielding 0.000494 g/L.
24	NaHCO3 CHO3.Na 84.006 g/mol	4.446640 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 124 contributes 4.50 g NaHCO3 in a 1012 mL final formulation, yielding 4.446640 g/L.
25	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.355731 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 124 adds all 10 mL of Archived DSMZ Medium 124 Solution B per 1012 mL final formulation, yielding 0.355731 g/L Na2S x 9 H2O.
26	Ethanol C2H6O 46.069 g/mol	20.000 MILLIMOLAR	CHEBI:16236 ethanol	Archived DSMZ Medium 313 uses DSMZ Medium 124 with 20 mM ethanol as the substrate.
27	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 124 lists 1000 mL distilled water for Solution A.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 313
2026-03-13T06:19:29.310795Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004988
2026-03-17T19:45:44.869564+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ACETOBACTERIUM CARBINOLICUM medium" to "acetobacterium_carbinolicum_medium" for consistent matching
2026-08-06T08:58:32.381777+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_313_score35.py	RESOLVED_KOMODO_313_SCORE35	Archived DSMZ Medium 313 defines ACETOBACTERIUM CARBINOLICUM MEDIUM as DSMZ Medium 124 with 20 mM ethanol as the substrate; this record expands the archived DSMZ Medium 124 base, Solution B, trace stock, and vitamin stock into final per-liter components.