methanogenium_aggregans_medium_replace_sludge_fluid_medium_119_with_rumen_fluid_clarified

CultureMech:005011 archaea COMPLEX LIQUID
Original name: METHANOGENIUM AGGREGANS medium_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified

Overview

External ontology
komodo.medium:321_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified (source link unavailable) — METHANOGENIUM AGGREGANS medium_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: KOMODO ModelSEED | ID: 321_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified | DSMZ Medium: 321 (mediadive.medium:321) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_005011["methanogenium_aggregans_medium_replace_sludge_fluid_medium_119_with..."]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_005011 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_005011 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005011 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Na_formate["Na-formate"]
    CultureMech_005011 --> ing3_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing3_Na_formate -.-> CHEBI_62965
    ing4_Na_acetate["Na-acetate"]
    CultureMech_005011 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_005011 --> ing5_Trypticase_peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_005011 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_005011 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_005011 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_005011 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005011 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_KH2PO4["KH2PO4"]
    CultureMech_005011 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005011 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946
    ing13_NaCl["NaCl"]
    CultureMech_005011 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005011 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005011 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_005011 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005011 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005011 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_005011 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005011 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005011 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_005011 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_H3BO3["H3BO3"]
    CultureMech_005011 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005011 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005011 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005011 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_005011 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing28_Sludge["Sludge"]
    CultureMech_005011 --> ing28_Sludge

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 Na-formate
CHO2.Na 68.007 g/mol
5 G_PER_L CHEBI:62965
sodium formate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
6 Trypticase peptone 1 G_PER_L
7 Yeast extract 5.0 G_PER_L [Merged 2 duplicates: 1.0, 4.0]
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
9 Na2CO3
CO3.2Na 105.988 g/mol
1.5 G_PER_L CHEBI:29377
sodium carbonate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.2 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 (NH4)2SO4
2H4N.O4S 132.141 g/mol
6 G_PER_L CHEBI:62946
ammonium sulfate
14 NaCl
Cl.Na 58.443 g/mol
13.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 12.0, 1.0]
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 2.6, 3.0]
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.26 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.16, 0.1]
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
29 Sludge 1000 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 321_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified
2026-02-03T11:19:15.538410 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 321
2026-03-13T03:09:33.305817Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:29.477208Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005011
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:45.054046+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOGENIUM AGGREGANS medium_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified" to "methanogenium_aggregans_medium_replace_sludge_fluid_medium_119_with_rumen_fluid_clarified" for consistent matching
2026-06-05T07:41:31.502348+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.969152+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.