anaerobic_acetoin_medium (CultureMech:005020)

Overview

External ontology

komodo.medium:327 (source link unavailable) — ANAEROBIC ACETOIN medium

Applications

Microbial cultivation

pH

7.2

Notes

Archived DSMZ Medium 327 defines ANAEROBIC ACETOIN medium as DSMZ Medium 213 with 1.5 g/L acetoin replacing butyrate; Medium 213 is Medium 212 prepared without Na2SO4. This record expands archived DSMZ Media 212, 213, 327, and 320 into final per-liter components.

Composition

flowchart LR
    CultureMech_005020["anaerobic_acetoin_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_005020 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005020 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NaCl["NaCl"]
    CultureMech_005020 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_005020 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005020 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_HCl["HCl"]
    CultureMech_005020 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_005020 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_ZnCl2["ZnCl2"]
    CultureMech_005020 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005020 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_005020 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005020 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005020 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005020 --> ing12_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005020 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Trypticase["Trypticase"]
    CultureMech_005020 --> ing14_Trypticase
    ing15_Rumen_fluid__clarified["Rumen fluid, clarified"]
    CultureMech_005020 --> ing15_Rumen_fluid__clarified
    ing16_Resazurin["Resazurin"]
    CultureMech_005020 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806
    ing17_NaHCO3["NaHCO3"]
    CultureMech_005020 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_Acetoin["Acetoin"]
    CultureMech_005020 --> ing18_Acetoin
    CHEBI_15688["CHEBI:15688"]
    ing18_Acetoin -.-> CHEBI_15688
    ing19_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_005020 --> ing19_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing19_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing20_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005020 --> ing20_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing20_Na2S_x_9_H2O -.-> CHEBI_76209
    ing21_Biotin["Biotin"]
    CultureMech_005020 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005020 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005020 --> ing23_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005020 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Pantothenic_acid["Pantothenic acid"]
    CultureMech_005020 --> ing25_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing25_Pantothenic_acid -.-> CHEBI_7916
    ing26_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005020 --> ing26_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_HCl -.-> CHEBI_30961
    ing27_CO2["CO2"]
    CultureMech_005020 --> ing27_CO2
    CHEBI_16526["CHEBI:16526"]
    ing27_CO2 -.-> CHEBI_16526
    ing28_N2["N2"]
    CultureMech_005020 --> ing28_N2
    CHEBI_17997["CHEBI:17997"]
    ing28_N2 -.-> CHEBI_17997
    ing29_Distilled_water["Distilled water"]
    CultureMech_005020 --> ing29_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing29_Distilled_water -.-> CHEBI_15377

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.497018 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 212 adds 50 mL of its mineral solution per 1006 mL final formulation; the mineral stock contains 10.00 g/L KH2PO4, yielding 0.497018 g/L.
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.328032 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 212 adds 50 mL of its mineral solution per 1006 mL final formulation; the mineral stock contains 6.60 g/L MgCl2 x 6 H2O, yielding 0.328032 g/L.
3	NaCl Cl.Na 58.443 g/mol	0.397614 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 212 adds 50 mL of its mineral solution per 1006 mL final formulation; the mineral stock contains 8.00 g/L NaCl, yielding 0.397614 g/L.
4	NH4Cl Cl.H4N 53.492 g/mol	0.397614 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 212 adds 50 mL of its mineral solution per 1006 mL final formulation; the mineral stock contains 8.00 g/L NH4Cl, yielding 0.397614 g/L.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.049702 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 212 adds 50 mL of its mineral solution per 1006 mL final formulation; the mineral stock contains 1.00 g/L CaCl2 x 2 H2O, yielding 0.049702 g/L.
6	HCl HCl 36.461 g/mol	0.002485 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002485 g/L.
7	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001491 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001491 g/L.
8	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000696 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000696 g/L.
9	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000994 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000994 g/L.
10	H3BO3 H3BO3 61.833 g/mol	0.00000596 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000596 g/L.
11	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000189 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000189 g/L.
12	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000199 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000199 g/L.
13	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000239 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000239 g/L.
14	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000358 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 212 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1006 mL final formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000358 g/L.
15	Trypticase	0.994036 G_PER_L		Archived DSMZ Medium 212 contributes 1.00 g Trypticase to the 1006 mL final formulation, yielding 0.994036 g/L.
16	Rumen fluid, clarified	49.701789 ML_PER_L		Archived DSMZ Medium 212 contributes 50 mL clarified rumen fluid to the 1006 mL final formulation, yielding 49.701789 mL/L.
17	Resazurin C12H7NO4 229.191 g/mol	0.000994 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 212 contributes 1 mg Resazurin to the 1006 mL final formulation, yielding 0.000994 g/L.
18	NaHCO3 CHO3.Na 84.006 g/mol	3.479125 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 212 contributes 3.50 g NaHCO3 to the 1006 mL final formulation, yielding 3.479125 g/L.
19	Acetoin C4H8O2 88.106 g/mol	1.500000 G_PER_L	CHEBI:15688 acetoin	Archived DSMZ Medium 327 replaces the butyrate in Medium 213 with 1.5 g/L acetoin.
20	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.298211 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 212 contributes 0.30 g Cysteine-HCl x H2O to the 1006 mL final formulation, yielding 0.298211 g/L.
21	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.298211 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 212 contributes 0.30 g Na2S x 9 H2O to the 1006 mL final formulation, yielding 0.298211 g/L.
22	Biotin C10H16N2O3S 244.316 g/mol	0.00000124 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 0.25 mg/L Biotin, yielding 0.00000124 g/L.
23	Nicotinic acid C6H5NO2 123.111 g/mol	0.0000124 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 2.50 mg/L Nicotinic acid, yielding 0.0000124 g/L.
24	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.00000621 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 1.25 mg/L Thiamine-HCl x 2 H2O, yielding 0.00000621 g/L.
25	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.00000621 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 1.25 mg/L p-Aminobenzoic acid, yielding 0.00000621 g/L.
26	Pantothenic acid C9H17NO5 219.237 g/mol	0.00000308 G_PER_L	CHEBI:7916 pantothenic acid	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 0.62 mg/L Pantothenic acid, yielding 0.00000308 g/L.
27	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.0000308 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 212 adds 5 mL of its vitamin solution per 1006 mL final formulation; the vitamin stock contains 6.20 mg/L Pyridoxine-HCl, yielding 0.0000308 g/L.
28	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 212 prepares solutions A and B under 80% N2 and 20% CO2.
29	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 212 prepares the medium under 80% N2 and 20% CO2.
30	Distilled water H2O 18.015 g/mol	900.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 212 lists 900 mL direct distilled water across solutions A-D before adding mineral, trace, vitamin, and rumen-fluid stocks.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 327
2026-03-13T06:19:29.572985Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005020
2026-03-17T19:45:45.162727+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ANAEROBIC ACETOIN medium" to "anaerobic_acetoin_medium" for consistent matching
2026-08-06T08:58:32.384705+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_327_score35.py	RESOLVED_KOMODO_327_SCORE35	Archived DSMZ Medium 327 defines ANAEROBIC ACETOIN medium as DSMZ Medium 213 with 1.5 g/L acetoin replacing butyrate; Medium 213 is Medium 212 prepared without Na2SO4. This record expands archived DSMZ Media 212, 213, 327, and 320 into final per-liter components.