Overview
- External ontology
- komodo.medium:330 (source link unavailable) — RUMEN BACTERIA medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.7, 'max': 6.8}
- Notes
- Source: KOMODO ModelSEED | ID: 330 | DSMZ Medium: 330 (mediadive.medium:330) | Aerobic: Yes Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve exact displayed Gram Amounts and nulls as source-specific assertions. Rounded molar columns and aggregated SEED rows are not ingredient evidence. No growth association is inferred. Retain source-specific pH; a null variant or stock pH does not inherit the base pH. Complete inspected source rows define this reconstruction. Archive old flattened quantities, false LB expansions, placeholders and copied stock-solvent claims in append-only history. Unrelated annotations remain preserved, not recertified.
Composition
flowchart LR
CultureMech_005024["rumen_bacteria_medium"]
ing0_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005024 --> ing0_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing0_Na2S_x_9_H2O -.-> CHEBI_76209
ing1_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_005024 --> ing1_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing1_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing2_Haemin["Haemin"]
CultureMech_005024 --> ing2_Haemin
CHEBI_50385["CHEBI:50385"]
ing2_Haemin -.-> CHEBI_50385
ing3_CO2["CO2"]
CultureMech_005024 --> ing3_CO2
ing4_K2HPO4["K2HPO4"]
CultureMech_005024 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_005024 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Glucose["Glucose"]
CultureMech_005024 --> ing6_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_Glucose -.-> CHEBI_17634
ing7_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_005024 --> ing7_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing7_iso_Butyric_acid -.-> CHEBI_16135
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005024 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_Butyric_acid["Butyric acid"]
CultureMech_005024 --> ing9_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing9_Butyric_acid -.-> CHEBI_30772
ing10_Yeast_extract["Yeast extract"]
CultureMech_005024 --> ing10_Yeast_extract
ing11_Cellobiose["Cellobiose"]
CultureMech_005024 --> ing11_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing11_Cellobiose -.-> CHEBI_17057
ing12_Resazurin["Resazurin"]
CultureMech_005024 --> ing12_Resazurin
ing13_Trypticase_Peptone__BBL_["Trypticase Peptone (BBL)"]
CultureMech_005024 --> ing13_Trypticase_Peptone__BBL_
ing14__NH4_2SO4["(NH4)2SO4"]
CultureMech_005024 --> ing14__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing14__NH4_2SO4 -.-> CHEBI_62946
ing15_Na2CO3["Na2CO3"]
CultureMech_005024 --> ing15_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing15_Na2CO3 -.-> CHEBI_29377
ing16_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_005024 --> ing16_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing16_iso_Valeric_acid -.-> CHEBI_28484
ing17_Propionic_acid["Propionic acid"]
CultureMech_005024 --> ing17_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing17_Propionic_acid -.-> CHEBI_30768
ing18_Distilled_water["Distilled water"]
CultureMech_005024 --> ing18_Distilled_water
ing19_DL_2_Methyl_butyric_acid["DL-2-Methyl butyric acid"]
CultureMech_005024 --> ing19_DL_2_Methyl_butyric_acid
ing20_Glycerol["Glycerol"]
CultureMech_005024 --> ing20_Glycerol
CHEBI_17754["CHEBI:17754"]
ing20_Glycerol -.-> CHEBI_17754
ing21_Maltose["Maltose"]
CultureMech_005024 --> ing21_Maltose
CHEBI_17306["CHEBI:17306"]
ing21_Maltose -.-> CHEBI_17306
ing22_n_Valeric_acid["n-Valeric acid"]
CultureMech_005024 --> ing22_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing22_n_Valeric_acid -.-> CHEBI_17418
ing23_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005024 --> ing23_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing23_CaCl2_x_2_H2O -.-> CHEBI_86158
ing24_NaCl["NaCl"]
CultureMech_005024 --> ing24_NaCl
CHEBI_26710["CHEBI:26710"]
ing24_NaCl -.-> CHEBI_26710
ing25_Starch__soluble["Starch, soluble"]
CultureMech_005024 --> ing25_Starch__soluble
CHEBI_28017["CHEBI:28017"]
ing25_Starch__soluble -.-> CHEBI_28017
ing26_Acetic_acid["Acetic acid"]
CultureMech_005024 --> ing26_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing26_Acetic_acid -.-> CHEBI_15366
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 2 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 3 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
9.99E-4 G_PER_L |
CHEBI:50385
hemin
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 4 | CO2 | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.30 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.23 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
0.50 G_PER_L |
CHEBI:17634
D-glucose
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 8 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
CHEBI:16135
isobutyric acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.09 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 10 |
Butyric acid
C4H8O2 88.106 g/mol
|
CHEBI:30772
butyric acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 11 | Yeast extract | 0.50 G_PER_L | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 12 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.50 G_PER_L |
CHEBI:17057
cellobiose
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 13 | Resazurin | 9.99E-4 G_PER_L | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. The source says Resazurin, not sodium resazurin. Withdraw copied salt-specific grounding; no replacement chemical identifier is inferred. | |
| 14 | Trypticase Peptone (BBL) | 2.00 G_PER_L | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 15 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.23 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 16 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4.00 G_PER_L |
CHEBI:29377
sodium carbonate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 17 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
CHEBI:28484
isovaleric acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 18 |
Propionic acid
C3H6O2 74.079 g/mol
|
CHEBI:30768
propionic acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 19 | Distilled water | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 20 | DL-2-Methyl butyric acid | Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 21 |
Glycerol
C3H8O3 92.094 g/mol
|
0.50 G_PER_L |
CHEBI:17754
glycerol
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 22 |
Maltose
C12H22O11 342.297 g/mol
|
0.50 G_PER_L |
CHEBI:17306
maltose
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 23 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
CHEBI:17418
valeric acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 24 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 25 |
NaCl
Cl.Na 58.443 g/mol
|
0.46 G_PER_L |
CHEBI:26710
sodium chloride
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 26 | Starch, soluble | 0.50 G_PER_L |
CHEBI:28017
starch
|
(soluble) Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
| 27 |
Acetic acid
C2H4O2 60.052 g/mol
|
CHEBI:15366
acetic acid
|
Exact displayed Gram Amount precision/nulls only; not rounded Mol Amount or combined SEED metabolites. Even rumen-fluid grams are a provider assertion, not a conversion from DSMZ ml/l. Exact heading: Gram & Mol amounts are per Liter of medium. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 330 |
| 2026-02-03T11:19:19.385193 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 330 |
| 2026-03-13T06:19:29.615105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005024 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.211102+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMEN BACTERIA medium" to "rumen_bacteria_medium" for consistent matching |
| 2026-05-20T06:00:51.226666+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.7-6.8' -> {'min': 6.7, 'max': 6.8} |
| 2026-06-05T07:40:22.496588+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.602510+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T08:40:02.418657+00:00 | codex | RESTORED_RUMEN_BACTERIA_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore source-specific medium330 ingredients, stock scopes and input units with literal evidence. Preserve prior supported entries, provider nulls, unknown original bases and the JCM1029 access gap. No growth association added. |