Overview
- External ontology
- komodo.medium:332 (source link unavailable) — METHANOGENIUM BOURGENSE medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.7, 'max': 7.0}
- Notes
- Source: KOMODO ModelSEED | ID: 332 | DSMZ Medium: 332 (mediadive.medium:332) | Aerobic: Yes
Composition
flowchart LR
CultureMech_005026["methanogenium_bourgense_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_005026 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_005026 --> ing1_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005026 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Na_formate["Na-formate"]
CultureMech_005026 --> ing3_Na_formate
CHEBI_62965["CHEBI:62965"]
ing3_Na_formate -.-> CHEBI_62965
ing4_Na_acetate["Na-acetate"]
CultureMech_005026 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Trypticase_peptone["Trypticase peptone"]
CultureMech_005026 --> ing5_Trypticase_peptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_005026 --> ing6_Yeast_extract
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_005026 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005026 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2CO3["Na2CO3"]
CultureMech_005026 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005026 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Na-formate
CHO2.Na 68.007 g/mol
|
5 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Trypticase peptone | 1 G_PER_L | ||
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 332 |
| 2026-02-03T11:19:01.825973 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 332 |
| 2026-03-13T06:19:29.636437Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005026 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.235256+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOGENIUM BOURGENSE medium" to "methanogenium_bourgense_medium" for consistent matching |
| 2026-05-20T06:01:55.669124+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.7-7.0' -> {'min': 6.7, 'max': 7.0} |
| 2026-06-05T07:41:31.513132+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.975036+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |