Overview
- External ontology
- komodo.medium:334b (source link unavailable) — METHANOSAETA HARUNDINACEA medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: KOMODO ModelSEED | ID: 334b | DSMZ Medium: 334b (mediadive.medium:334b) | Aerobic: No
Composition
flowchart LR
CultureMech_005029["methanosaeta_harundinacea_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005029 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_005029 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_Na_acetate["Na-acetate"]
CultureMech_005029 --> ing2_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Na_acetate -.-> CHEBI_32954
ing3_Na2_EDTA["Na2-EDTA"]
CultureMech_005029 --> ing3_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing3_Na2_EDTA -.-> CHEBI_64734
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_005029 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_005029 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_NaCl["NaCl"]
CultureMech_005029 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005029 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005029 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_NH4Cl["NH4Cl"]
CultureMech_005029 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_Butyl_vinyl_ether["Butyl vinyl ether"]
CultureMech_005029 --> ing10_Butyl_vinyl_ether
ing11_Methanol["Methanol"]
CultureMech_005029 --> ing11_Methanol
CHEBI_17790["CHEBI:17790"]
ing11_Methanol -.-> CHEBI_17790
ing12_L___Phosphatidylcholine["L-α-Phosphatidylcholine"]
CultureMech_005029 --> ing12_L___Phosphatidylcholine
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005029 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_005029 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_005029 --> ing15_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing15_FeCl3_x_6_H2O -.-> CHEBI_86254
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005029 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005029 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_ZnCl2["ZnCl2"]
CultureMech_005029 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_CuCl2["CuCl2"]
CultureMech_005029 --> ing19_CuCl2
CHEBI_49553["CHEBI:49553"]
ing19_CuCl2 -.-> CHEBI_49553
ing20_H3BO3["H3BO3"]
CultureMech_005029 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005029 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005029 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_005029 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Biotin["Biotin"]
CultureMech_005029 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_005029 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_005029 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_005029 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_005029 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_005029 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_005029 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.233546 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.912951 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
7.21868 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 4 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.00106157 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000530786 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
31.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 30.0, 1.0] |
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
11.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 11.0, 0.1] |
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
30 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 11 | Butyl vinyl ether | 5 G_PER_L | ||
| 12 |
Methanol
CH4O 32.042 g/mol
|
792.0 G_PER_L |
CHEBI:17790
methanol
|
[Collapsed 2 identical duplicates: 792.0, 792.0] |
| 13 | L-α-Phosphatidylcholine | 5 G_PER_L | ||
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
50 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.03 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.03 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 334b |
| 2026-02-03T11:19:00.435297 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 334 |
| 2026-02-06T05:10:21.731858Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:52.904293Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:29.670273Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005029 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.274027+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSAETA HARUNDINACEA medium" to "methanosaeta_harundinacea_medium" for consistent matching |
| 2026-05-20T06:01:56.144787+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.5' -> {'min': 7.2, 'max': 7.5} |
| 2026-06-05T07:41:32.207373+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.227297+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:30.650790+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |