Overview
- External ontology
- komodo.medium:345.2 (source link unavailable) — For DSM 3246
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.5}
- Notes
- DSMZ and MediaDive now curate DSM 3246 as DSMZ Medium 298 with strain-specific modifications, replacing 2,3-butanediol with 1 g/L Acetoin.
Composition
flowchart LR
CultureMech_005056["for_dsm_3246"]
ing0_Na2CO3["Na2CO3"]
CultureMech_005056 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaCl["NaCl"]
CultureMech_005056 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Acetoin["Acetoin"]
CultureMech_005056 --> ing2_Acetoin
CHEBI_15688["CHEBI:15688"]
ing2_Acetoin -.-> CHEBI_15688
ing3_KCl["KCl"]
CultureMech_005056 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005056 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005056 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_NH4Cl["NH4Cl"]
CultureMech_005056 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_KH2PO4["KH2PO4"]
CultureMech_005056 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005056 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_HCl["HCl"]
CultureMech_005056 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_005056 --> ing10_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
ing11_Sodium_resazurin["Sodium resazurin"]
CultureMech_005056 --> ing11_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Sodium_resazurin -.-> CHEBI_8806
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005056 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005056 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_ZnCl2["ZnCl2"]
CultureMech_005056 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005056 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005056 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_H3BO3["H3BO3"]
CultureMech_005056 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005056 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 3 |
Acetoin
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:15688
acetoin
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.36 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 10 |
HCl
HCl 36.461 g/mol
|
0.0025 G_PER_L |
CHEBI:17883
hydrogen chloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 11 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.0015 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 12 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.00019 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0001 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
7e-05 G_PER_L |
CHEBI:49976
zinc dichloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3.6e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
2.4e-05 G_PER_L |
CHEBI:34887
nickel dichloride
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
6e-06 G_PER_L |
CHEBI:33118
boric acid
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2e-06 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
MediaDive lists this final concentration for the DSM 3246 strain-specific DSMZ Medium 298 formulation. |
Preparation
- Prepare the DSM 3246 variant of anaerobic DSMZ Medium 298 by omitting 2,3-butanediol and adding 1 g/L Acetoin.
- Sparge the medium with 80% N2 and 20% CO2 for 30 to 45 minutes, dispense under the same gas atmosphere into anoxic tubes or vials, and autoclave.
- Add Acetoin and sulfide from sterile anoxic stock solutions after autoclaving.
- Adjust the complete medium to pH 7.2 to 7.5 before use.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Syntrophotalea acetylenica DSM 3246
- Term
- Id
- NCBITaxon:29542
- Label
- Syntrophotalea acetylenica
- Strain
- WoAcy 1; DSM 3246; NBRC 103808
- Evidence
- Reference
- https://bacdive.dsmz.de/strain/4213
- Supports
- SUPPORT
- Explanation
- BacDive lists Syntrophotalea acetylenica DSM 3246 as cultivated on PELOBACTER PROPIONICUS MEDIUM (FRESHWATER), DSMZ Medium 298, and MediaDive lists the 1 g/L Acetoin strain modification at 30 C under anaerobic conditions.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 345.2 |
| 2026-03-13T06:19:29.834772Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005056 |
| 2026-03-17T19:45:45.471567+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 3246" to "for_dsm_3246" for consistent matching |
| 2026-08-06T08:58:32.436501+00:00 | curate_flag_empty_composition.py | FLAGGED_INCOMPLETE_COMPOSITION | Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175). |
| 2026-09-06T00:00:00-07:00 | repair_bacterial_strain_specific_score45.py | RESOLVED_STRAIN_SPECIFIC_SCORE45_GRAPH | DSMZ and MediaDive now curate DSM 3246 as DSMZ Medium 298 with strain-specific modifications, replacing 2,3-butanediol with 1 g/L Acetoin. |