acetobacterium_2_medium (CultureMech:005062)

Overview

External ontology

komodo.medium:349 (source link unavailable) — ACETOBACTERIUM 2 medium

Applications

Microbial cultivation

Notes

Archived DSMZ Medium 349 defines ACETOBACTERIUM 2 MEDIUM as DSMZ Medium 135 supplemented with 20 g/L NaCl and with 1.25 g/L ethylene glycol replacing fructose; this record expands the archived DSMZ Medium 135 base and Medium 141 trace and vitamin stocks into final per-liter components.

Composition

flowchart LR
    CultureMech_005062["acetobacterium_2_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_005062 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005062 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_005062 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005062 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_005062 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005062 --> ing5_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_x_H2O -.-> CHEBI_86364
    ing6_NaCl["NaCl"]
    CultureMech_005062 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005062 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_005062 --> ing8_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing8_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005062 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005062 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005062 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_005062 --> ing12_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_005062 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005062 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005062 --> ing15_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005062 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_Biotin["Biotin"]
    CultureMech_005062 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_005062 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005062 --> ing19_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_HCl -.-> CHEBI_30961
    ing20_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005062 --> ing20_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing20_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing21_Riboflavin["Riboflavin"]
    CultureMech_005062 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005062 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_005062 --> ing23_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_D_Ca_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_005062 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005062 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_Lipoic_acid["Lipoic acid"]
    CultureMech_005062 --> ing26_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26_Lipoic_acid -.-> CHEBI_16494
    ing27_Yeast_extract["Yeast extract"]
    CultureMech_005062 --> ing27_Yeast_extract
    ing28_Resazurin["Resazurin"]
    CultureMech_005062 --> ing28_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing28_Resazurin -.-> CHEBI_8806
    ing29_NaHCO3["NaHCO3"]
    CultureMech_005062 --> ing29_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing29_NaHCO3 -.-> CHEBI_32139
    CultureMech_005062 --> more["...4 more"]

Ingredients (34)

#	Ingredient	Concentration	Ontology	Notes
1	NH4Cl Cl.H4N 53.492 g/mol	0.961538 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 135 contributes 0.961538 g/L NH4Cl after scaling the 1040 mL final formulation to one liter.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.317308 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 135 contributes 0.317308 g/L KH2PO4 after scaling the 1040 mL final formulation to one liter.
3	K2HPO4 HO4P.2K 174.174 g/mol	0.432692 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 135 contributes 0.432692 g/L K2HPO4 after scaling the 1040 mL final formulation to one liter.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.153846 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 349 inherits 0.10 g MgSO4 x 7 H2O from Medium 135 and 20 mL of a 3.00 g/L Medium 141 trace stock per 1040 mL final formulation, yielding 0.153846 g/L total.
5	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.028846 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.028846 g/L Nitrilotriacetic acid.
6	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.009615 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.009615 g/L MnSO4 x H2O.
7	NaCl Cl.Na 58.443 g/mol	20.019231 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 349 adds 20 g/L NaCl and Archived DSMZ Medium 135 adds 20 mL of a 1.00 g/L Medium 141 trace stock per 1040 mL final formulation, yielding 20.019231 g/L total.
8	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.001923 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.001923 g/L FeSO4 x 7 H2O.
9	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.003462 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.003462 g/L CoSO4 x 7 H2O.
10	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.001923 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.001923 g/L CaCl2 x 2 H2O.
11	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.003462 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.003462 g/L ZnSO4 x 7 H2O.
12	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.000192 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.000192 g/L CuSO4 x 5 H2O.
13	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000385 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.000385 g/L KAl(SO4)2 x 12 H2O.
14	H3BO3 H3BO3 61.833 g/mol	0.000192 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.000192 g/L H3BO3.
15	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000192 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.000192 g/L Na2MoO4 x 2 H2O.
16	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000577 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.000577 g/L NiCl2 x 6 H2O.
17	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.00000577 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 trace element solution per 1040 mL final formulation, yielding 0.00000577 g/L Na2SeO3 x 5 H2O.
18	Biotin C10H16N2O3S 244.316 g/mol	0.000038 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000038 g/L Biotin.
19	Folic acid C19H19N7O6 441.404 g/mol	0.000038 G_PER_L	CHEBI:27470 folic acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000038 g/L Folic acid.
20	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000192 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000192 g/L Pyridoxine-HCl.
21	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000096 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L Thiamine-HCl x 2 H2O.
22	Riboflavin C17H20N4O6 376.369 g/mol	0.000096 G_PER_L	CHEBI:17015 riboflavin	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L Riboflavin.
23	Nicotinic acid C6H5NO2 123.111 g/mol	0.000096 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L Nicotinic acid.
24	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000096 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L D-Ca-pantothenate.
25	Vitamin B12	0.00000192 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.00000192 g/L Vitamin B12.
26	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000096 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L p-Aminobenzoic acid.
27	Lipoic acid C8H14O2S2 206.332 g/mol	0.000096 G_PER_L	CHEBI:16494 lipoic acid	Archived DSMZ Medium 135 adds 20 mL of the Archived DSMZ Medium 141 vitamin solution per 1040 mL final formulation, yielding 0.000096 g/L Lipoic acid.
28	Yeast extract	1.923077 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 135 contributes 1.923077 g/L Yeast extract after scaling the 1040 mL final formulation to one liter.
29	Resazurin C12H7NO4 229.191 g/mol	0.000962 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 135 contributes 0.000962 g/L Resazurin after scaling the 1040 mL final formulation to one liter.
30	NaHCO3 CHO3.Na 84.006 g/mol	9.615385 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 135 contributes 9.615385 g/L NaHCO3 after scaling the 1040 mL final formulation to one liter.
31	Ethylene glycol C2H6O2 62.068 g/mol	1.250000 G_PER_L	CHEBI:30742 ethylene glycol	Archived DSMZ Medium 349 replaces fructose with 1.25 g/L ethylene glycol.
32	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.480769 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 135 contributes 0.480769 g/L Cysteine-HCl x H2O after scaling the 1040 mL final formulation to one liter.
33	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.480769 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 135 contributes 0.480769 g/L Na2S x 9 H2O after scaling the 1040 mL final formulation to one liter.
34	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 135 lists 1000 mL distilled water.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 349
2026-03-13T06:19:29.886954Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005062
2026-03-17T19:45:45.533531+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ACETOBACTERIUM 2 medium" to "acetobacterium_2_medium" for consistent matching
2026-08-06T08:58:32.380491+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_349_score35.py	RESOLVED_KOMODO_349_SCORE35	Archived DSMZ Medium 349 defines ACETOBACTERIUM 2 MEDIUM as DSMZ Medium 135 supplemented with 20 g/L NaCl and with 1.25 g/L ethylene glycol replacing fructose; this record expands the archived DSMZ Medium 135 base and Medium 141 trace and vitamin stocks into final per-liter components.