Overview
- External ontology
- komodo.medium:354 (source link unavailable) — L?WENSTEIN-JENSEN medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 354 | DSMZ Medium: 354 (mediadive.medium:354) | Aerobic: No
Composition
flowchart LR
CultureMech_005071["l_wenstein_jensen_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005071 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4["MgSO4"]
CultureMech_005071 --> ing1_MgSO4
CHEBI_32599["CHEBI:32599"]
ing1_MgSO4 -.-> CHEBI_32599
ing2_Mg_citrate["Mg-citrate"]
CultureMech_005071 --> ing2_Mg_citrate
CHEBI_131389["CHEBI:131389"]
ing2_Mg_citrate -.-> CHEBI_131389
ing3_L_Asparagin["L-Asparagin"]
CultureMech_005071 --> ing3_L_Asparagin
CHEBI_17196["CHEBI:17196"]
ing3_L_Asparagin -.-> CHEBI_17196
ing4_Potato_flour["Potato flour"]
CultureMech_005071 --> ing4_Potato_flour
ing5_Malachite_green["Malachite green"]
CultureMech_005071 --> ing5_Malachite_green
CHEBI_72449["CHEBI:72449"]
ing5_Malachite_green -.-> CHEBI_72449
ing6_Glycerol["Glycerol"]
CultureMech_005071 --> ing6_Glycerol
CHEBI_17754["CHEBI:17754"]
ing6_Glycerol -.-> CHEBI_17754
ing7_Fresh_egg_mixture["Fresh egg mixture"]
CultureMech_005071 --> ing7_Fresh_egg_mixture
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.55087 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.148883 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 3 |
Mg-citrate
C6H6O7.Mg 214.412 g/mol
|
0.372208 G_PER_L |
CHEBI:131389
magnesium citrate
|
|
| 4 |
L-Asparagin
C4H8N2O3 132.119 g/mol
|
2.23325 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 5 | Potato flour | 18.6104 G_PER_L | ||
| 6 |
Malachite green
C23H25N2.Cl 364.92 g/mol
|
0.248139 G_PER_L |
CHEBI:72449
malachite green
|
|
| 7 |
Glycerol
C3H8O3 92.094 g/mol
|
12 G_PER_L |
CHEBI:17754
glycerol
|
|
| 8 | Fresh egg mixture | 1000 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 354 |
| 2026-02-03T11:19:06.853397 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 354 |
| 2026-03-13T06:19:29.939851Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005071 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.596157+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "L?WENSTEIN-JENSEN medium" to "l_wenstein_jensen_medium" for consistent matching |
| 2026-06-05T07:40:22.677072+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.673173+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T22:47:39.592416+00:00 | codex | CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 354 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants. |