methanosarcina_frisia_medium (CultureMech:005072)

Overview

External ontology

komodo.medium:355 (source link unavailable) — METHANOSARCINA FRISIA medium

Applications

Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Archived DSMZ Medium 355 defines METHANOSARCINA FRISIA MEDIUM as DSMZ Medium 141 prepared under 80:20 N2/CO2, with separately sterilized methanol added to 0.2% (v/v) and final pH 6.8-7.0; this record expands DSMZ Medium 141 trace and vitamin stocks into final per-liter components.

Composition

flowchart LR
    CultureMech_005072["methanosarcina_frisia_medium"]
    ing0_KCl["KCl"]
    CultureMech_005072 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005072 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005072 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_005072 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005072 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_005072 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_005072 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Fe_NH4_2_SO4_2_x_7_H2O["Fe(NH4)2(SO4)2 x 7 H2O"]
    CultureMech_005072 --> ing7_Fe_NH4_2_SO4_2_x_7_H2O
    CHEBI_131378["CHEBI:131378"]
    ing7_Fe_NH4_2_SO4_2_x_7_H2O -.-> CHEBI_131378
    ing8_NaHCO3["NaHCO3"]
    CultureMech_005072 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Na_acetate["Na-acetate"]
    CultureMech_005072 --> ing9_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Na_acetate -.-> CHEBI_32954
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_005072 --> ing10_Yeast_extract
    ing11_Trypticase["Trypticase"]
    CultureMech_005072 --> ing11_Trypticase
    ing12_Resazurin["Resazurin"]
    CultureMech_005072 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_005072 --> ing13_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005072 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_005072 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005072 --> ing16_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_x_H2O -.-> CHEBI_86364
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005072 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_005072 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005072 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005072 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_005072 --> ing21_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_H3BO3["H3BO3"]
    CultureMech_005072 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005072 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005072 --> ing24_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005072 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Biotin["Biotin"]
    CultureMech_005072 --> ing26_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing26_Biotin -.-> CHEBI_15956
    ing27_Folic_acid["Folic acid"]
    CultureMech_005072 --> ing27_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing27_Folic_acid -.-> CHEBI_27470
    ing28_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005072 --> ing28_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing28_Pyridoxine_HCl -.-> CHEBI_30961
    ing29_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005072 --> ing29_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing29_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    CultureMech_005072 --> more["...11 more"]

Ingredients (41)

#	Ingredient	Concentration	Ontology	Notes
1	KCl Cl.K 74.551 g/mol	0.333333 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 141 lists 0.34 g KCl and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.333333 g/L in the 1020 mL aqueous formulation.
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	3.921569 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 141 lists 4.00 g MgCl2 x 6 H2O and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 3.921569 g/L in the 1020 mL aqueous formulation.
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	3.411765 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 141 lists 3.45 g MgSO4 x 7 H2O in the main recipe and adds 10 mL of a trace solution containing 3.00 g/L MgSO4 x 7 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 3.411765 g/L in the 1020 mL aqueous formulation.
4	NH4Cl Cl.H4N 53.492 g/mol	0.245098 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 141 lists 0.25 g NH4Cl and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.245098 g/L in the 1020 mL aqueous formulation.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.138235 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 141 lists 0.14 g CaCl2 x 2 H2O in the main recipe and adds 10 mL of a trace solution containing 0.10 g/L CaCl2 x 2 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.138235 g/L in the 1020 mL aqueous formulation.
6	K2HPO4 HO4P.2K 174.174 g/mol	0.137255 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 141 lists 0.14 g K2HPO4 and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.137255 g/L in the 1020 mL aqueous formulation.
7	NaCl Cl.Na 58.443 g/mol	17.656863 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 141 lists 18.00 g NaCl in the main recipe and adds 10 mL of a trace solution containing 1.00 g/L NaCl to 1000 mL water plus 10 mL vitamin solution, yielding 17.656863 g/L in the 1020 mL aqueous formulation.
8	Fe(NH4)2(SO4)2 x 7 H2O Fe.7H2O.2H4N.2O4S 410.154 g/mol	0.001961 G_PER_L	CHEBI:131378 Fe(NH4)2(SO4)2 x 7 H2O	Archived DSMZ Medium 141 lists 2.00 mg Fe(NH4)2(SO4)2 x 7 H2O and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.001961 g/L in the 1020 mL aqueous formulation.
9	NaHCO3 CHO3.Na 84.006 g/mol	4.901961 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 141 lists 5.00 g NaHCO3 and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 4.901961 g/L in the 1020 mL aqueous formulation.
10	Na-acetate C2H3O2.Na 82.034 g/mol	0.980392 G_PER_L	CHEBI:32954 Na-acetate	Archived DSMZ Medium 141 lists 1.00 g Na-acetate and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.980392 g/L in the 1020 mL aqueous formulation.
11	Yeast extract	1.960784 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 141 lists 2.00 g Yeast extract and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 1.960784 g/L in the 1020 mL aqueous formulation.
12	Trypticase	1.960784 G_PER_L	MICRO:0000175 (source link unavailable) Trypticase	Archived DSMZ Medium 141 lists 2.00 g Trypticase and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 1.960784 g/L in the 1020 mL aqueous formulation.
13	Resazurin C12H7NO4 229.191 g/mol	0.000980 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 141 lists 1.00 mg Resazurin and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.000980 g/L in the 1020 mL aqueous formulation.
14	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.490196 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 141 lists 0.50 g Cysteine-HCl x H2O and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.490196 g/L in the 1020 mL aqueous formulation.
15	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.490196 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 141 lists 0.50 g Na2S x 9 H2O and adds 10 mL trace solution and 10 mL vitamin solution to 1000 mL water, yielding 0.490196 g/L in the 1020 mL aqueous formulation.
16	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.014706 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 1.50 g/L Nitrilotriacetic acid to 1000 mL water plus 10 mL vitamin solution, yielding 0.014706 g/L in the 1020 mL aqueous formulation.
17	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.004902 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.50 g/L MnSO4 x H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.004902 g/L in the 1020 mL aqueous formulation.
18	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.000980 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.10 g/L FeSO4 x 7 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000980 g/L in the 1020 mL aqueous formulation.
19	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.001765 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.18 g/L CoSO4 x 7 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.001765 g/L in the 1020 mL aqueous formulation.
20	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.001765 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.18 g/L ZnSO4 x 7 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.001765 g/L in the 1020 mL aqueous formulation.
21	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.000098 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.01 g/L CuSO4 x 5 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000098 g/L in the 1020 mL aqueous formulation.
22	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000196 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.02 g/L KAl(SO4)2 x 12 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000196 g/L in the 1020 mL aqueous formulation.
23	H3BO3 H3BO3 61.833 g/mol	0.000098 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.01 g/L H3BO3 to 1000 mL water plus 10 mL vitamin solution, yielding 0.000098 g/L in the 1020 mL aqueous formulation.
24	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000098 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.01 g/L Na2MoO4 x 2 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000098 g/L in the 1020 mL aqueous formulation.
25	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000294 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.03 g/L NiCl2 x 6 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000294 g/L in the 1020 mL aqueous formulation.
26	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.000002941 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 141 adds 10 mL of a trace solution containing 0.30 mg/L Na2SeO3 x 5 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000002941 g/L in the 1020 mL aqueous formulation.
27	Biotin C10H16N2O3S 244.316 g/mol	0.000019608 G_PER_L	CHEBI:15956 Biotin	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 2.00 mg/L Biotin to 1000 mL water plus 10 mL trace solution, yielding 0.000019608 g/L in the 1020 mL aqueous formulation.
28	Folic acid C19H19N7O6 441.404 g/mol	0.000019608 G_PER_L	CHEBI:27470 Folic acid	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 2.00 mg/L Folic acid to 1000 mL water plus 10 mL trace solution, yielding 0.000019608 g/L in the 1020 mL aqueous formulation.
29	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000098 G_PER_L	CHEBI:30961 Pyridoxine hydrochloride	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 10.00 mg/L Pyridoxine-HCl to 1000 mL water plus 10 mL trace solution, yielding 0.000098 g/L in the 1020 mL aqueous formulation.
30	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000049 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L Thiamine-HCl x 2 H2O to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
31	Riboflavin C17H20N4O6 376.369 g/mol	0.000049 G_PER_L	CHEBI:17015 Riboflavin	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L Riboflavin to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
32	Nicotinic acid C6H5NO2 123.111 g/mol	0.000049 G_PER_L	CHEBI:15940 Nicotinic acid	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L Nicotinic acid to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
33	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000049 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L D-Ca-pantothenate to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
34	Vitamin B12	0.000000980 G_PER_L	CHEBI:176843 Vitamin B12	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 0.10 mg/L Vitamin B12 to 1000 mL water plus 10 mL trace solution, yielding 0.000000980 g/L in the 1020 mL aqueous formulation.
35	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000049 G_PER_L	CHEBI:30753 p-Aminobenzoic acid	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L p-Aminobenzoic acid to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
36	(DL)-alpha-Lipoic acid C8H14O2S2 206.332 g/mol	0.000049 G_PER_L	CHEBI:16494 (DL)-alpha-Lipoic acid	Archived DSMZ Medium 141 adds 10 mL of a vitamin solution containing 5.00 mg/L Lipoic acid to 1000 mL water plus 10 mL trace solution, yielding 0.000049 g/L in the 1020 mL aqueous formulation.
37	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	Archived DSMZ Medium 141 adjusts the trace element solution with KOH.
38	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 355 prepares DSMZ Medium 141 under 80% N2 plus 20% CO2.
39	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 355 prepares DSMZ Medium 141 under 80% N2 plus 20% CO2.
40	Methanol CH4O 32.042 g/mol	0.200000 PERCENT_V_V	CHEBI:17790 methanol	Archived DSMZ Medium 355 adds separately sterilized methanol to the medium to a final concentration of 0.2% v/v.
41	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 141 lists 1000 mL distilled water before stock additions.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 355
2026-03-13T06:19:29.942291Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005072
2026-03-17T19:45:45.599035+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOSARCINA FRISIA medium" to "methanosarcina_frisia_medium" for consistent matching
2026-05-20T06:01:56.295312+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '6.8-7.0' -> {'min': 6.8, 'max': 7.0}
2026-08-06T08:58:32.369761+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_355_score30.py	RESOLVED_KOMODO_355_SCORE30	Archived DSMZ Medium 355 defines METHANOSARCINA FRISIA MEDIUM as DSMZ Medium 141 prepared under 80:20 N2/CO2, with separately sterilized methanol added to 0.2% (v/v) and final pH 6.8-7.0; this record expands DSMZ Medium 141 trace and vitamin stocks into final per-liter components.