Overview
- External ontology
- komodo.medium:35a.1 (source link unavailable) — For DSM 22701
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: KOMODO ModelSEED | ID: 35a.1 | DSMZ Medium: 35a (mediadive.medium:35a) | Aerobic: No
Composition
flowchart LR
CultureMech_005080["for_dsm_22701"]
ing0_NH4Cl["NH4Cl"]
CultureMech_005080 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_005080 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Agar["Agar"]
CultureMech_005080 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005080 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005080 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_005080 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Yeast_extract["Yeast extract"]
CultureMech_005080 --> ing6_Yeast_extract
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.111111 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.33333 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 | Agar | 22.2222 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
250 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 | Yeast extract | 100 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 35a.1 |
| 2026-02-03T11:18:56.998624 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 35 |
| 2026-03-13T06:19:29.988967Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005080 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.653162+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 22701" to "for_dsm_22701" for consistent matching |
| 2026-05-20T06:01:19.311658+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.5-6.0' -> {'min': 5.5, 'max': 6.0} |
| 2026-06-05T07:41:03.859155+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.210111+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_35_thiobacillus_topology_score10.py | RESOLVED_KOMODO_35_THIOBACILLUS_TOPOLOGY | KOMODO Medium 35a.1 preserves THIOBACILLUS THIOOXIDANS MEDIUM components and concentrations but records pH 6 for DSM 22701. |