Overview
- External ontology
- komodo.medium:371 (source link unavailable) — NATRONOBACTERIA medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 9.0, 'max': 9.5}
- High-metal flag
- True
- Notes
- Source: KOMODO ModelSEED | ID: 371 | DSMZ Medium: 371 (mediadive.medium:371) | Aerobic: No
Composition
flowchart LR
CultureMech_005093["natronobacteria_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005093 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_KCl["KCl"]
CultureMech_005093 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_NH4Cl["NH4Cl"]
CultureMech_005093 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005093 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_005093 --> ing4_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
ing5_Agar["Agar"]
CultureMech_005093 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_NaCl["NaCl"]
CultureMech_005093 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Na2_glutamate["Na2-glutamate"]
CultureMech_005093 --> ing7_Na2_glutamate
CHEBI_64220["CHEBI:64220"]
ing7_Na2_glutamate -.-> CHEBI_64220
ing8_Yeast_extract["Yeast extract"]
CultureMech_005093 --> ing8_Yeast_extract
ing9_Casamino_acids["Casamino acids"]
CultureMech_005093 --> ing9_Casamino_acids
ing10_Na2CO3["Na2CO3"]
CultureMech_005093 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_HCl["HCl"]
CultureMech_005093 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_005093 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_ZnCl2["ZnCl2"]
CultureMech_005093 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005093 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_005093 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005093 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005093 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005093 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005093 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.24 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.17 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
Na2-glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 9 | Yeast extract | 5 G_PER_L | ||
| 10 | Casamino acids | 5 G_PER_L | ||
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 371 |
| 2026-02-03T11:19:04.376553 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 371 |
| 2026-03-13T06:19:30.052280Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005093 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.730539+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRONOBACTERIA medium" to "natronobacteria_medium" for consistent matching |
| 2026-05-20T06:00:51.433874+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.0-9.5' -> {'min': 9.0, 'max': 9.5} |
| 2026-06-05T07:40:22.774201+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.928439+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:44.700923+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |