thermophilic_methanosaeta_medium (CultureMech:005164)

Overview

External ontology

komodo.medium:387 (source link unavailable) — THERMOPHILIC METHANOSAETA MEDIUM

Applications

Microbial cultivation

pH

6.5

Notes

Archived DSMZ Medium 387 defines THERMOPHILIC METHANOSAETA MEDIUM as 940 mL distilled water with NH4Cl, K2HPO4, MgCl2, resazurin, and DSMZ Medium 141 trace elements, followed after autoclaving by anoxic NaHCO3, CaCl2, Na-acetate, DSMZ Medium 141 vitamins, Coenzyme M, and Na2S stocks; this record expands the trace and vitamin stocks into final per-liter components after the 1.015 L final volume.

Composition

flowchart LR
    CultureMech_005164["thermophilic_methanosaeta_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_005164 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4["K2HPO4"]
    CultureMech_005164 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005164 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Resazurin["Resazurin"]
    CultureMech_005164 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005164 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_005164 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_005164 --> ing6_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005164 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005164 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_005164 --> ing9_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing9_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005164 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005164 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_005164 --> ing12_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_005164 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005164 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005164 --> ing15_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005164 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_KOH["KOH"]
    CultureMech_005164 --> ing17_KOH
    CHEBI_32035["CHEBI:32035"]
    ing17_KOH -.-> CHEBI_32035
    ing18_NaHCO3["NaHCO3"]
    CultureMech_005164 --> ing18_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing18_NaHCO3 -.-> CHEBI_32139
    ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005164 --> ing19_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing20_Na_acetate["Na-acetate"]
    CultureMech_005164 --> ing20_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing20_Na_acetate -.-> CHEBI_32954
    ing21_Biotin["Biotin"]
    CultureMech_005164 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_005164 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005164 --> ing23_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_HCl -.-> CHEBI_30961
    ing24_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005164 --> ing24_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing24_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing25_Riboflavin["Riboflavin"]
    CultureMech_005164 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005164 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_005164 --> ing27_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_005164 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005164 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_005164 --> more["...6 more"]

Ingredients (36)

#	Ingredient	Concentration	Ontology	Notes
1	NH4Cl Cl.H4N 53.492 g/mol	0.492611 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 387 lists 0.5 g NH4Cl in 940 mL water with 10 mL trace elements and 65 mL of post-autoclave stocks, yielding 0.492611 g/L in the 1015 mL final formulation.
2	K2HPO4 HO4P.2K 174.174 g/mol	0.394089 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 387 lists 0.4 g K2HPO4 in 940 mL water with 10 mL trace elements and 65 mL of post-autoclave stocks, yielding 0.394089 g/L in the 1015 mL final formulation.
3	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.098522 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 387 lists 0.1 g MgCl2 x 6 H2O in 940 mL water with 10 mL trace elements and 65 mL of post-autoclave stocks, yielding 0.098522 g/L in the 1015 mL final formulation.
4	Resazurin C12H7NO4 229.191 g/mol	0.000493 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 387 lists 0.5 mg Resazurin in 940 mL water with 10 mL trace elements and 65 mL of post-autoclave stocks, yielding 0.000493 g/L in the 1015 mL final formulation.
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.029557 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 3.00 g/L MgSO4 x 7 H2O, yielding 0.029557 g/L in the 1015 mL final formulation.
6	NaCl Cl.Na 58.443 g/mol	0.009852 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 1.00 g/L NaCl, yielding 0.009852 g/L in the 1015 mL final formulation.
7	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.014778 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 1.50 g/L Nitrilotriacetic acid, yielding 0.014778 g/L in the 1015 mL final formulation.
8	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.004926 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.50 g/L MnSO4 x H2O, yielding 0.004926 g/L in the 1015 mL final formulation.
9	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.000985 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.10 g/L FeSO4 x 7 H2O, yielding 0.000985 g/L in the 1015 mL final formulation.
10	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.001773 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.18 g/L CoSO4 x 7 H2O, yielding 0.001773 g/L in the 1015 mL final formulation.
11	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.001773 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.18 g/L ZnSO4 x 7 H2O, yielding 0.001773 g/L in the 1015 mL final formulation.
12	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.000099 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.01 g/L CuSO4 x 5 H2O, yielding 0.000099 g/L in the 1015 mL final formulation.
13	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000197 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.02 g/L KAl(SO4)2 x 12 H2O, yielding 0.000197 g/L in the 1015 mL final formulation.
14	H3BO3 H3BO3 61.833 g/mol	0.000099 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.01 g/L H3BO3, yielding 0.000099 g/L in the 1015 mL final formulation.
15	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000099 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.01 g/L Na2MoO4 x 2 H2O, yielding 0.000099 g/L in the 1015 mL final formulation.
16	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000296 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.03 g/L NiCl2 x 6 H2O, yielding 0.000296 g/L in the 1015 mL final formulation.
17	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.000002956 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 trace elements containing 0.30 mg/L Na2SeO3 x 5 H2O, yielding 0.000002956 g/L in the 1015 mL final formulation.
18	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	Archived DSMZ Medium 141 adjusts the trace element solution with KOH; Archived DSMZ Medium 387 adds 10 mL of that trace solution.
19	NaHCO3 CHO3.Na 84.006 g/mol	0.985222 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 387 adds 20 mL of an anoxic sterile 5% w/v NaHCO3 stock per liter medium after autoclaving, yielding 0.985222 g/L in the 1015 mL final formulation.
20	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.099507 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 387 adds 10 mL of 1% w/v CaCl2 x 2 H2O stock and 10 mL of Archived DSMZ Medium 141 trace elements containing 0.10 g/L CaCl2 x 2 H2O, yielding 0.099507 g/L in the 1015 mL final formulation.
21	Na-acetate C2H3O2.Na 82.034 g/mol	3.251232 G_PER_L	CHEBI:32954 Na-acetate	Archived DSMZ Medium 387 adds 10 mL of an anoxic sterile 33% w/v Na-acetate stock per liter medium after autoclaving, yielding 3.251232 g/L in the 1015 mL final formulation.
22	Biotin C10H16N2O3S 244.316 g/mol	0.000019704 G_PER_L	CHEBI:15956 Biotin	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 2.00 mg/L Biotin, yielding 0.000019704 g/L in the 1015 mL final formulation.
23	Folic acid C19H19N7O6 441.404 g/mol	0.000019704 G_PER_L	CHEBI:27470 Folic acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 2.00 mg/L Folic acid, yielding 0.000019704 g/L in the 1015 mL final formulation.
24	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000098522 G_PER_L	CHEBI:30961 Pyridoxine hydrochloride	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 10.00 mg/L Pyridoxine-HCl, yielding 0.000098522 g/L in the 1015 mL final formulation.
25	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000049261 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L Thiamine-HCl x 2 H2O, yielding 0.000049261 g/L in the 1015 mL final formulation.
26	Riboflavin C17H20N4O6 376.369 g/mol	0.000049261 G_PER_L	CHEBI:17015 Riboflavin	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L Riboflavin, yielding 0.000049261 g/L in the 1015 mL final formulation.
27	Nicotinic acid C6H5NO2 123.111 g/mol	0.000049261 G_PER_L	CHEBI:15940 Nicotinic acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L Nicotinic acid, yielding 0.000049261 g/L in the 1015 mL final formulation.
28	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000049261 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L D-Ca-pantothenate, yielding 0.000049261 g/L in the 1015 mL final formulation.
29	Vitamin B12	0.000000985 G_PER_L	CHEBI:176843 Vitamin B12	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 0.10 mg/L Vitamin B12, yielding 0.000000985 g/L in the 1015 mL final formulation.
30	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000049261 G_PER_L	CHEBI:30753 p-Aminobenzoic acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L p-Aminobenzoic acid, yielding 0.000049261 g/L in the 1015 mL final formulation.
31	(DL)-alpha-Lipoic acid C8H14O2S2 206.332 g/mol	0.000049261 G_PER_L	CHEBI:16494 (DL)-alpha-Lipoic acid	Archived DSMZ Medium 387 adds 10 mL of Archived DSMZ Medium 141 vitamin solution containing 5.00 mg/L Lipoic acid, yielding 0.000049261 g/L in the 1015 mL final formulation.
32	Coenzyme M C2H6O3S2 142.201 g/mol	0.139901 G_PER_L	CHEBI:17905 coenzyme M	Archived DSMZ Medium 387 adds 10 mL of an anoxic sterile 1.42% w/v Coenzyme M stock per liter medium after autoclaving, yielding 0.139901 g/L in the 1015 mL final formulation.
33	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.246305 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 387 adds 5 mL of an anoxic sterile 5% w/v Na2S x 9 H2O stock per liter medium after autoclaving, yielding 0.246305 g/L in the 1015 mL final formulation.
34	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 387 cools and dispenses the medium under an 80% N2 plus 20% CO2 atmosphere before adding extra CO2.
35	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 387 cools and dispenses the medium under 80:20 N2/CO2 and then adds CO2 by syringe to bring the gas atmosphere to 30% CO2.
36	Distilled water H2O 18.015 g/mol	940.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 387 lists 940 mL distilled water before stock additions.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 387
2026-03-13T06:19:30.736847Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005164
2026-03-17T19:45:46.533713+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "THERMOPHILIC METHANOSAETA MEDIUM" to "thermophilic_methanosaeta_medium" for consistent matching
2026-08-06T08:58:32.373074+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_387_score30.py	RESOLVED_KOMODO_387_SCORE30	Archived DSMZ Medium 387 defines THERMOPHILIC METHANOSAETA MEDIUM as 940 mL distilled water with NH4Cl, K2HPO4, MgCl2, resazurin, and DSMZ Medium 141 trace elements, followed after autoclaving by anoxic NaHCO3, CaCl2, Na-acetate, DSMZ Medium 141 vitamins, Coenzyme M, and Na2S stocks; this record expands the trace and vitamin stocks into final per-liter components after the 1.015 L final volume.