Overview
- External ontology
- komodo.medium:389 (source link unavailable) — THIOBACILLUS AQUAESULIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: KOMODO ModelSEED | ID: 389 | DSMZ Medium: 389 (mediadive.medium:389) | Aerobic: No
Composition
flowchart LR
CultureMech_005166["thiobacillus_aquaesulis_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_005166 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_005166 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_005166 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005166 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Agar["Agar"]
CultureMech_005166 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_005166 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_005166 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005166 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005166 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_005166 --> ing9__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing9__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_005166 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_NaOH["NaOH"]
CultureMech_005166 --> ing11_NaOH
CHEBI_32145["CHEBI:32145"]
ing11_NaOH -.-> CHEBI_32145
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
79 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
15 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.45977 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.114943 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Agar | 17.2414 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
250 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
7.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.5 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 10 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.5 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 |
NaOH
HO.Na 39.997 g/mol
|
11 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Solutions
Trace element solution
Dose: 10 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 11 G_PER_L | |
| MnCl2 x 4 H2O | 2.5 G_PER_L | |
| FeSO4 x 7 H2O | 5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 389 |
| 2026-02-03T11:19:15.631616 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 389 |
| 2026-03-13T06:19:30.753714Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005166 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:46.552373+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THIOBACILLUS AQUAESULIS MEDIUM" to "thiobacillus_aquaesulis_medium" for consistent matching |
| 2026-06-05T07:41:51.175387+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.397865+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:31.986921+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-08-15T05:35:05.379256+00:00 | promote_komodo_volume_candidates.py | ASSERTED_VOLUME_FROM_COMPOSITION_MATCH | This record reproduces MediaDive medium 389 ('ANNWOODIA MEDIUM') across 15 shared compounds at 100% identical values. That identifies the medium despite the name difference, so its stated addition volume is now asserted rather than proposed (#262). |