Overview
- External ontology
- komodo.medium:398.2 (source link unavailable) — Aerobic version
- Applications
-
- Microbial cultivation
- pH
- 2.0
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 398.2 | DSMZ Medium: 398 (mediadive.medium:398) | Aerobic: Yes
Composition
flowchart LR
CultureMech_005186["aerobic_version"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005186 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005186 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005186 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_005186 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Yeast_extract["Yeast extract"]
CultureMech_005186 --> ing4_Yeast_extract
ing5_Glucose["Glucose"]
CultureMech_005186 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Sulfur["Sulfur"]
CultureMech_005186 --> ing6_Sulfur
CHEBI_26833["CHEBI:26833"]
ing6_Sulfur -.-> CHEBI_26833
ing7_H2SO4["H2SO4"]
CultureMech_005186 --> ing7_H2SO4
CHEBI_26836["CHEBI:26836"]
ing7_H2SO4 -.-> CHEBI_26836
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 | Yeast extract | 1 G_PER_L | ||
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 |
Sulfur
S 32.067 g/mol
|
4 G_PER_L |
CHEBI:26833
sulfur atom
|
(for anaerobic media only) |
| 8 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 398.2 |
| 2026-02-03T11:18:58.766754 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 398 |
| 2026-03-13T03:08:48.677470Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: H2SO4 |
| 2026-03-13T06:19:30.934633Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005186 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:46.756477+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Aerobic version" to "aerobic_version" for consistent matching |
| 2026-05-16T09:03:23.406212+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:47.535025+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.680180+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_398_thermoplasma_score10.py | RESOLVED_KOMODO_398_THERMOPLASMA_TOPOLOGY | KOMODO Medium 398.2 mirrors DSMZ Medium 398 with sulfuric acid represented as the variable pH-adjustment ingredient. |