salt_solution_medium_592

CultureMech:005192 bacterial DEFINED LIQUID
Original name: Salt solution (medium 592)

Overview

External ontology
komodo.medium:4002 (source link unavailable) — Salt solution (medium 592)
Applications
  • Microbial cultivation
pH
2.0
Notes
KOMODO Medium 4002 is a SubMedium import labeled Salt solution (medium 592). The DSMZ Medium 592 PDF has one embedded stock adjusted to pH 2.0 with H2SO4, Trace elements, prepared in 100.000 ml distilled water; this repair uses that stock formula. The local unrelated mediadive.solution:4002 Main sol. J298 record was not used.

Composition

flowchart LR
    CultureMech_005192["salt_solution_medium_592"]
    ing0_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005192 --> ing0_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing0_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing1_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_005192 --> ing1_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing1_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing2_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005192 --> ing2_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing2_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing3_CuCl2_x_H2O["CuCl2 x H2O"]
    CultureMech_005192 --> ing3_CuCl2_x_H2O
    CHEBI_49553["CHEBI:49553"]
    ing3_CuCl2_x_H2O -.-> CHEBI_49553
    ing4_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005192 --> ing4_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing5_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_005192 --> ing5_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing5_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing6_Distilled_water["Distilled water"]
    CultureMech_005192 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_H2SO4["H2SO4"]
    CultureMech_005192 --> ing7_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing7_H2SO4 -.-> CHEBI_26836

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
18 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 DSMZ Medium 592 Trace elements lists 1.800 g MnCl2 x 4 H2O per 100.000 ml; concentration scaled to per-litre units.
2 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
44 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
 DSMZ Medium 592 Trace elements lists 4.400 g Na2B4O7 x 10 H2O per 100.000 ml; concentration scaled to per-litre units.
3 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
2.2 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 DSMZ Medium 592 Trace elements lists 0.220 g ZnSO4 x 7 H2O per 100.000 ml; concentration scaled to per-litre units.
4 CuCl2 x H2O
Cl2Cu 134.452 g/mol
0.5 G_PER_L CHEBI:49553
copper(II) chloride
 DSMZ Medium 592 Trace elements lists 0.050 g CuCl2 x H2O per 100.000 ml; concentration scaled to per-litre units.
5 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.3 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 DSMZ Medium 592 Trace elements lists 0.030 g Na2MoO4 x 2 H2O per 100.000 ml; concentration scaled to per-litre units.
6 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
0.3 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
 DSMZ Medium 592 Trace elements lists 0.030 g VOSO4 x 2 H2O per 100.000 ml; concentration scaled to per-litre units.
7 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 592 Trace elements lists distilled water brought to 100.000 ml.
8 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 DSMZ Medium 592 Trace elements instructs adjustment to pH 2.0 with H2SO4 and gives no amount.

Preparation

  1. Dissolve the trace-elements stock components in distilled water and bring to 100.000 ml.
  2. Adjust to pH 2.0 with H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 4002
2026-03-13T06:19:30.967115Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005192
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:46.794228+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Salt solution (medium 592)" to "salt_solution_medium_592" for consistent matching
2026-05-16T09:04:31.271343+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:45.191411+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_komodo_4002_salt_solution_592_score15.py RESOLVED_KOMODO_4002_SALT_SOLUTION_592_SCORE15 KOMODO Medium 4002 is a SubMedium import labeled Salt solution (medium 592). The DSMZ Medium 592 PDF has one embedded stock adjusted to pH 2.0 with H2SO4, Trace elements, prepared in 100.000 ml distilled water; this repair uses that stock formula. The local unrelated mediadive.solution:4002 Main sol. J298 record was not used. Replaced the imported H2SO4-only pH-buffer stub with the complete DSMZ trace-elements stock formula, explicit water, the variable H2SO4 pH-adjustment row, and DSMZ preparation steps.