columbia_blood_agar

Overview

External ontology

komodo.medium:429 (source link unavailable) — COLUMBIA BLOOD AGAR

Applications

• Microbial cultivation

Notes

Source: KOMODO ModelSEED | ID: 429 | DSMZ Medium: 429 (mediadive.medium:429) | Aerobic: No Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_005268["columbia_blood_agar"]
    ing0_Columbia_agar_base["Columbia agar base"]
    CultureMech_005268 --> ing0_Columbia_agar_base
    ing1_Horse_blood["Horse blood"]
    CultureMech_005268 --> ing1_Horse_blood
    ing2_Casein_peptone["Casein peptone"]
    CultureMech_005268 --> ing2_Casein_peptone
    ing3_Soy_peptone["Soy peptone"]
    CultureMech_005268 --> ing3_Soy_peptone
    ing4_D____Glucose["D(+)-Glucose"]
    CultureMech_005268 --> ing4_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing4_D____Glucose -.-> CHEBI_17634
    ing5_NaCl["NaCl"]
    CultureMech_005268 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_K2HPO4["K2HPO4"]
    CultureMech_005268 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Calf_brains["Calf brains"]
    CultureMech_005268 --> ing7_Calf_brains
    ing8_Beef_heart["Beef heart"]
    CultureMech_005268 --> ing8_Beef_heart
    ing9_Proteose_peptone["Proteose peptone"]
    CultureMech_005268 --> ing9_Proteose_peptone
    ing10_Dextrose["Dextrose"]
    CultureMech_005268 --> ing10_Dextrose
    ing10_Dextrose -.-> CHEBI_17634
    ing11_Sodium_chloride["Sodium chloride"]
    CultureMech_005268 --> ing11_Sodium_chloride
    ing11_Sodium_chloride -.-> CHEBI_26710
    ing12_Disodium_phosphate["Disodium phosphate"]
    CultureMech_005268 --> ing12_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing12_Disodium_phosphate -.-> CHEBI_34683
    ing13_Peptone["Peptone"]
    CultureMech_005268 --> ing13_Peptone
    ing14_Meat_extract["Meat extract"]
    CultureMech_005268 --> ing14_Meat_extract

Ingredients (15)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Staphylococcus saccharolyticus

  Term

  Id

  NCBITaxon:33028

  Label

  Staphylococcus saccharolyticus

  Genome assembly id

  • GCA_003968885.1
  • SAMN05977977

  Strain

  ATCC 14953; DSM 20359; JCM 1768; NCTC 11807; VPI 5661; S1

  Evidence

  • Reference

    https://bacdive.dsmz.de/strain/14567

    Supports

    SUPPORT

    Explanation

    BacDive lists the type strain aliases, NCBI taxon 33028, Columbia Blood Medium cultivation, and positive growth at 37 C for this strain.

  Growth metrics

  • Temperature celsius

    37.0

    Measurement conditions

    Columbia blood agar; anaerobic incubation; qualitative positive growth/count evidence rather than OD or doubling-time measurement.

    Evidence

    • Reference

      PMID:7077295

      Supports

      SUPPORT

      Snippet

      The highest bacterial counts were obtained on Columbia blood agar incubated anaerobically.

      Explanation

      The abstract directly supports growth of Peptococcus saccharolyticus, now Staphylococcus saccharolyticus, on Columbia blood agar under anaerobic incubation.

    • Reference

      https://bacdive.dsmz.de/strain/14567

      Supports

      SUPPORT

      Explanation

      BacDive strain 14567 links the ATCC 14953 / DSM 20359 / NCTC 11807 type strain to positive growth on Columbia Blood Medium at 37 C.

    Is max attainment

    False

Recipe variants

• Name

  columbia_blood_agar_staphylococcus_saccharolyticus_anaerobic

  Description

  Columbia blood agar cultivation evidence for the type strain Staphylococcus saccharolyticus ATCC 14953 / DSM 20359 / NCTC 11807, formerly Peptococcus saccharolyticus.

  Modifications

  • BacDive DSMZ Medium 693 specifies Columbia agar base with 50 g/L defibrinated sheep blood; the local parent record contains a Columbia blood agar base with horse blood at 40 g/L.
  • Incubate anaerobically or under reduced oxygen at 37 C for this anaerobic organism.

  Purpose

  Capture strain-specific qualitative growth evidence while preserving the blood-source and concentration difference as a variant-level formulation note.

  Evidence

  • Reference

    PMID:7077295

    Supports

    SUPPORT

    Snippet

    The highest bacterial counts were obtained on Columbia blood agar incubated anaerobically.

    Explanation

    The primary literature reports quantitative culture of Peptococcus saccharolyticus strains, now Staphylococcus saccharolyticus, on Columbia blood agar under anaerobic incubation.

  • Reference

    https://bacdive.dsmz.de/strain/14567

    Supports

    SUPPORT

    Explanation

    BacDive identifies Staphylococcus saccharolyticus S1 as the type strain with DSM 20359, ATCC 14953, JCM 1768, and NCTC 11807 aliases; its cultivation data list Columbia Blood Medium with positive growth at 37 C.

Provenance