Overview
- External ontology
- komodo.medium:436_18848 (source link unavailable) — MEDIUM 436 MODIFIED FOR DSM 18848
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 436_18848 | DSMZ Medium: 436 (mediadive.medium:436) | Aerobic: No Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. KOMODO displayed Gram Amounts and nulls are source-specific assertions. Rounded molar columns and aggregated SEED entries are not ingredient evidence. No organism-growth association is inferred. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified.
Composition
flowchart LR
CultureMech_005283["medium_436_modified_for_dsm_18848"]
ing0_Na2CO3["Na2CO3"]
CultureMech_005283 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_Tryptone["Tryptone"]
CultureMech_005283 --> ing1_Tryptone
ing2_N2["N2"]
CultureMech_005283 --> ing2_N2
ing3_2_Methylbutyric_acid["2-Methylbutyric acid"]
CultureMech_005283 --> ing3_2_Methylbutyric_acid
ing4_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_005283 --> ing4_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Isovaleric_acid["Isovaleric acid"]
CultureMech_005283 --> ing5_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing5_Isovaleric_acid -.-> CHEBI_28484
ing6_CO2["CO2"]
CultureMech_005283 --> ing6_CO2
ing7_Distilled_water["Distilled water"]
CultureMech_005283 --> ing7_Distilled_water
ing8_K2HPO4["K2HPO4"]
CultureMech_005283 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_005283 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_Glucose["Glucose"]
CultureMech_005283 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_CaCl2_x_7_H2O["CaCl2 x 7 H2O"]
CultureMech_005283 --> ing11_CaCl2_x_7_H2O
CHEBI_3312["CHEBI:3312"]
ing11_CaCl2_x_7_H2O -.-> CHEBI_3312
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005283 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_maltose["maltose"]
CultureMech_005283 --> ing13_maltose
ing14_Isobutyric_acid["Isobutyric acid"]
CultureMech_005283 --> ing14_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing14_Isobutyric_acid -.-> CHEBI_16135
ing15_Yeast_extract["Yeast extract"]
CultureMech_005283 --> ing15_Yeast_extract
ing16_Cellobiose["Cellobiose"]
CultureMech_005283 --> ing16_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing16_Cellobiose -.-> CHEBI_17057
ing17_Resazurin["Resazurin"]
CultureMech_005283 --> ing17_Resazurin
CHEBI_8806["CHEBI:8806"]
ing17_Resazurin -.-> CHEBI_8806
ing18_NaCl["NaCl"]
CultureMech_005283 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_starch["starch"]
CultureMech_005283 --> ing19_starch
ing20__NH4_2SO4["(NH4)2SO4"]
CultureMech_005283 --> ing20__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing20__NH4_2SO4 -.-> CHEBI_62946
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4.00 G_PER_L |
CHEBI:29377
sodium carbonate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 2 | Tryptone | 5.00 G_PER_L | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 3 | N2 | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 4 | 2-Methylbutyric acid | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 5 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.50 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 6 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
CHEBI:28484
isovaleric acid
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 7 | CO2 | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 8 | Distilled water | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.24 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
3.00 G_PER_L |
CHEBI:17234
glucose
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 12 |
CaCl2 x 7 H2O
Ca.2Cl 110.984 g/mol
|
0.06 G_PER_L |
CHEBI:3312
calcium dichloride
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. Retain the literal seven-water source label. Other sources use dihydrate or hexahydrate labels; no chemical-form equivalence or molecular weight is inferred. Existing generic calcium-chloride annotation is not hydrate-specific evidence. |
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 14 | maltose | 1.00 G_PER_L | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 15 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
CHEBI:16135
isobutyric acid
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 16 | Yeast extract | 2.00 G_PER_L | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 17 |
Cellobiose
C12H22O11 342.297 g/mol
|
2.00 G_PER_L |
CHEBI:17057
cellobiose
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 18 |
Resazurin
C12H7NO4 229.191 g/mol
|
9.99E-4 G_PER_L |
CHEBI:8806
Resazurin
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
0.48 G_PER_L |
CHEBI:26710
sodium chloride
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
| 20 | starch | 1.00 G_PER_L | Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 21 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.80 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Exact displayed Gram Amount precision and nulls; never rounded Mol Amount or combined SEED metabolites Exact heading: Gram & Mol amounts are per Liter of medium. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 436_18848 |
| 2026-02-03T11:18:55.329038 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 436 |
| 2026-03-13T06:19:31.496660Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005283 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.384755+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 436 MODIFIED FOR DSM 18848" to "medium_436_modified_for_dsm_18848" for consistent matching |
| 2026-06-05T07:41:19.150565+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:02.716435+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T07:30:35.561516+00:00 | codex | RESTORED_RUMINOCOCCUS_ALBUS_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore exact-source medium436 formulation, preparation stages and input units. Preserve qualified prior rates, source-specific nulls and unresolved original bases. No growth association added. |