amb_medium (CultureMech:005305)

Overview

External ontology

komodo.medium:455 (source link unavailable) — AMB medium

Applications

Microbial cultivation

pH

6.9

Notes

Source: KOMODO ModelSEED | ID: 455 | DSMZ Medium: 455 (mediadive.medium:455) | Aerobic: No

Composition

flowchart LR
    CultureMech_005305["amb_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_005305 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_005305 --> ing1_Yeast_extract
    ing2_Starch["Starch"]
    CultureMech_005305 --> ing2_Starch
    CHEBI_28017["CHEBI:28017"]
    ing2_Starch -.-> CHEBI_28017
    ing3_Meat_infusion["Meat infusion"]
    CultureMech_005305 --> ing3_Meat_infusion
    ing4_Glucose["Glucose"]
    CultureMech_005305 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Cysteine["Cysteine"]
    CultureMech_005305 --> ing5_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing5_Cysteine -.-> CHEBI_15356
    ing6_CaCl2["CaCl2"]
    CultureMech_005305 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_MgSO4["MgSO4"]
    CultureMech_005305 --> ing7_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing7_MgSO4 -.-> CHEBI_32599
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005305 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_K2HPO4["K2HPO4"]
    CultureMech_005305 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	Trypticase peptone	4 G_PER_L
2	Yeast extract	5 G_PER_L
3	Starch	1 G_PER_L	CHEBI:28017 starch
4	Meat infusion	25 G_PER_L
5	Glucose C6H12O6 180.156 g/mol	5 G_PER_L	CHEBI:17234 glucose
6	Cysteine C3H7NO2S 121.161 g/mol	1 G_PER_L	CHEBI:15356 cysteine
7	CaCl2 Ca.2Cl 110.984 g/mol	0.01 G_PER_L	CHEBI:3312 calcium dichloride
8	MgSO4 Mg.O4S 120.368 g/mol	0.2 G_PER_L	CHEBI:32599 magnesium sulfate
9	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1 G_PER_L	CHEBI:62946 ammonium sulfate
10	K2HPO4 HO4P.2K 174.174 g/mol	15 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 455
2026-02-03T11:18:55.181726	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 455
2026-03-13T06:19:31.599391Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005305
2026-03-17T06:29:42.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:47.501593+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "AMB medium" to "amb_medium" for consistent matching
2026-06-05T07:40:23.418123+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.921557+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T22:47:39.592416+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 455 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.