medium_457_modified_for_dsm_11345

CultureMech:005318 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 457 MODIFIED FOR DSM 11345

Overview

External ontology
komodo.medium:457_11345 (source link unavailable) — MEDIUM 457 MODIFIED FOR DSM 11345
Applications
  • Microbial cultivation
Notes
Source: KOMODO ModelSEED | ID: 457_11345 | DSMZ Medium: 457 (mediadive.medium:457) | Aerobic: No Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_005318["medium_457_modified_for_dsm_11345"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_005318 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005318 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005318 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005318 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005318 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Dibenzofuran["Dibenzofuran"]
    CultureMech_005318 --> ing5_Dibenzofuran
    CHEBI_28145["CHEBI:28145"]
    ing5_Dibenzofuran -.-> CHEBI_28145
    ing6_Dimethyl_sulfoxide["Dimethyl sulfoxide"]
    CultureMech_005318 --> ing6_Dimethyl_sulfoxide
    CHEBI_28262["CHEBI:28262"]
    ing6_Dimethyl_sulfoxide -.-> CHEBI_28262
    ing7_Peptone["Peptone"]
    CultureMech_005318 --> ing7_Peptone
    ing8_Meat_extract["Meat extract"]
    CultureMech_005318 --> ing8_Meat_extract
    ing9_Agar["Agar"]
    CultureMech_005318 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_Casein_peptone["Casein peptone"]
    CultureMech_005318 --> ing10_Casein_peptone
    ing11_Soy_peptone["Soy peptone"]
    CultureMech_005318 --> ing11_Soy_peptone
    ing12_NaCl["NaCl"]
    CultureMech_005318 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_005318 --> ing13_Pancreatic_digest_of_casein
    ing14_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_005318 --> ing14_Peptic_digest_of_soybean_meal
    ing15_Glucose["Glucose"]
    CultureMech_005318 --> ing15_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing15_Glucose -.-> CHEBI_17234
    ing16_Sodium_chloride["Sodium chloride"]
    CultureMech_005318 --> ing16_Sodium_chloride
    ing16_Sodium_chloride -.-> CHEBI_26710
    ing17_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_005318 --> ing17_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing17_Dipotassium_phosphate -.-> CHEBI_131527
    ing18_Agar["Agar"]
    CultureMech_005318 --> ing18_Agar
    ing18_Agar -.-> CHEBI_2509
    ing19_EDTA["EDTA"]
    CultureMech_005318 --> ing19_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing19_EDTA -.-> CHEBI_4735
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005318 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005318 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005318 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_H3BO3["H3BO3"]
    CultureMech_005318 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005318 --> ing24_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005318 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005318 --> ing26_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005318 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
2.44 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.52 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Dibenzofuran
C12H8O 168.195 g/mol
8.4 G_PER_L CHEBI:28145
dibenzofuran
7 Dimethyl sulfoxide
C2H6OS 78.136 g/mol
1000 G_PER_L CHEBI:28262
dimethyl sulfoxide
8 Peptone 5 G_PER_L
9 Meat extract 3 G_PER_L
10 Agar 50.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Merged 3 duplicates: 15.0, 15.0, 20.0]
11 Casein peptone 15 G_PER_L
12 Soy peptone 5 G_PER_L
13 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
14 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
15 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
16 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
17 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
18 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
19 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
20 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
25 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
26 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
27 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 457_11345
2026-02-03T11:19:01.097813 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 457
2026-03-13T03:08:54.574529Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:31.718650Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005318
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:47.634931+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 457 MODIFIED FOR DSM 11345" to "medium_457_modified_for_dsm_11345" for consistent matching
2026-06-05T07:41:19.405826+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:02.796241+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.