for_dsm_13022

CultureMech:005458 bacterial COMPLEX LIQUID
Original name: For DSM 13022

Overview

External ontology
komodo.medium:457b.2 (source link unavailable) — For DSM 13022
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: KOMODO ModelSEED | ID: 457b.2 | DSMZ Medium: 457b (mediadive.medium:457b) | Aerobic: No

Composition

flowchart LR
    CultureMech_005458["for_dsm_13022"]
    ing0_Tween_80["Tween 80"]
    CultureMech_005458 --> ing0_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing0_Tween_80 -.-> CHEBI_53426
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_005458 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_KH2PO4["KH2PO4"]
    CultureMech_005458 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005458 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005458 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005458 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_EDTA["EDTA"]
    CultureMech_005458 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005458 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005458 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005458 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_005458 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005458 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005458 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005458 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005458 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Phenanthrene["Phenanthrene"]
    CultureMech_005458 --> ing15_Phenanthrene
    CHEBI_28851["CHEBI:28851"]
    ing15_Phenanthrene -.-> CHEBI_28851
    ing16_Casamino_acids__DIFCO_["Casamino acids (DIFCO)"]
    CultureMech_005458 --> ing16_Casamino_acids__DIFCO_

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.2 G_PER_L CHEBI:53426
polysorbate 80
2 Na2HPO4
HO4P.2Na 141.958 g/mol
2.44 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
1.52 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Phenanthrene
C14H10 178.234 g/mol
0.02 G_PER_L CHEBI:28851
phenanthrene
 KOMODO Medium 457b.2 lists 0.02 g/L phenanthrene for DSM 13022.
17 Casamino acids (DIFCO) 0.30 G_PER_L FOODON:03315719
mammalian milk protein (hydrolyzed)
 KOMODO Medium 457b.2 lists 0.30 g/L Casamino acids for DSM 13022.

Preparation

  1. DSMZ Medium 457b prepares a 2 g/L fluoranthene stock in acetone and filter-sterilizes it with a cellulose filter membrane.
  2. DSMZ Medium 457b adds 1 mL of the fluoranthene stock to a sterile culture flask, lets the acetone evaporate, and adds 20 mL of medium; direct addition of about 1-2 mg fluoranthene is also possible.
  3. DSMZ Medium 457 adjusts the mineral base to pH 6.9.
  4. DSMZ Medium 457 autoclaves the phosphate solution separately and combines it with the rest of the medium after cooling.
  5. DSMZ Medium 457b reactivates lyophilized cells in a complex medium before transferring them to mineral medium 457 with the appropriate carbon source.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 457b.2
2026-02-03T11:19:06.998656 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 457
2026-03-13T03:09:10.819086Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:33.088508Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005458
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:49.151175+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "For DSM 13022" to "for_dsm_13022" for consistent matching
2026-06-05T07:41:01.107041+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:57.132390+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_dsmz_457b_score15.py RESOLVED_DSMZ_KOMODO_457B_SCORE15 KOMODO Medium 457b.2 records the DSM 13022 formulation with phenanthrene substrate and Casamino acids.