nitrogen_free_medium_for_pseudomonas_stutzeri (CultureMech:005466)

Overview

External ontology

komodo.medium:460 (source link unavailable) — NITROGEN-FREE medium FOR PSEUDOMONAS STUTZERI

Applications

Microbial cultivation

pH

7.0

Notes

Source: KOMODO ModelSEED | ID: 460 | DSMZ Medium: 460 (mediadive.medium:460) | Aerobic: No

Composition

flowchart LR
    CultureMech_005466["nitrogen_free_medium_for_pseudomonas_stutzeri"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_005466 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005466 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_005466 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_005466 --> ing3_Yeast_extract
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_005466 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_DL_Na_malate["DL-Na-malate"]
    CultureMech_005466 --> ing5_DL_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing5_DL_Na_malate -.-> CHEBI_91260

Ingredients (6)

#	Ingredient	Concentration	Ontology
1	K2HPO4 HO4P.2K 174.174 g/mol	0.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
3	NaCl Cl.Na 58.443 g/mol	0.1 G_PER_L	CHEBI:26710 sodium chloride
4	Yeast extract	0.2 G_PER_L
5	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.015 G_PER_L	CHEBI:86254 iron trichloride hexahydrate
6	DL-Na-malate	6.6 G_PER_L	CHEBI:91260 disodium malate

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 460
2026-02-03T11:19:13.778326	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 460
2026-03-13T06:19:33.146658Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005466
2026-03-17T06:30:39.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:49.216620+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "NITROGEN-FREE medium FOR PSEUDOMONAS STUTZERI" to "nitrogen_free_medium_for_pseudomonas_stutzeri" for consistent matching
2026-06-05T07:40:23.512442+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.952375+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-08T00:00:39.369716+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 460 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.