mineral_medium_with_dichloromethane

CultureMech:005571 bacterial DEFINED LIQUID
Original name: MINERAL MEDIUM WITH DICHLOROMETHANE

Overview

External ontology
komodo.medium:465c (source link unavailable) — MINERAL MEDIUM WITH DICHLOROMETHANE
Applications
  • Microbial cultivation
pH
7.25
Notes
Source: KOMODO ModelSEED | ID: 465c | DSMZ Medium: 465c (mediadive.medium:465c) | Aerobic: No

Composition

flowchart LR
    CultureMech_005571["mineral_medium_with_dichloromethane"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_005571 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005571 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005571 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005571 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_005571 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Bromothymol_blue["Bromothymol blue"]
    CultureMech_005571 --> ing5_Bromothymol_blue
    CHEBI_86155["CHEBI:86155"]
    ing5_Bromothymol_blue -.-> CHEBI_86155
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_005571 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005571 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005571 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005571 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_005571 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005571 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005571 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005571 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005571 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
3.5 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.05 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
6 Bromothymol blue
C27H28Br2O5S 624.391 g/mol
0.05 G_PER_L CHEBI:86155
bromothymol blue
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.0005 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0002 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0001 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.0003 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0002 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.00001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00002 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00003 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare medium 465 with optional 50 mg/l bromothymol blue as pH indicator and autoclave. For DSM 6813, add 10 ml/l instead of 1 ml/l trace element solution SL-4.
  2. Grow cells in medium 465 with methanol supplied via incubation atmosphere up to 10 ml methanol per liter medium, then feed dichloromethane via the incubation atmosphere at 2 mmol/l or less.
  3. Maintain pH 7.25 and readjust the culture with sterile 1 M NaOH if necessary.
  4. Rehydrate lyophilized cells in the appropriate complex medium and then cultivate on mineral medium with the appropriate carbon source.
  5. For trace element solution SL-4, first dissolve EDTA in distilled water and adjust to pH 7.0 with 2 N NaOH, then add the other compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 465c
2026-02-03T11:19:04.921980 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 465
2026-03-13T03:09:04.834934Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:34.183361Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005571
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:50.381677+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM WITH DICHLOROMETHANE" to "mineral_medium_with_dichloromethane" for consistent matching
2026-06-05T07:40:23.725147+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:28.118946+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:45.026590+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_dsmz_465c_dichloromethane_score15.py RESOLVED_DSMZ_KOMODO_465C_SCORE15 Removed non-source complex-medium contaminant rows and resolved standard DSMZ Medium 465c trace element solution SL-4 dilution.