desulfobacterium_anilini_medium (CultureMech:005590)

Overview

External ontology

komodo.medium:476 (source link unavailable) — DESULFOBACTERIUM ANILINI MEDIUM

Applications

Microbial cultivation

pH range

{'min': 7.1, 'max': 7.4}

Notes

Archived DSMZ Medium 476 defines DESULFOBACTERIUM ANILINI MEDIUM as DSMZ Medium 193 with 1 mM phenol replacing sodium acetate, with 1 mL/L DSMZ Medium 385 selenite/tungsten solution, and with 1 mL/L DSMZ Medium 351 Seven Vitamins Solution; this record expands archived DSMZ Media 193, 320, 141, 385, and 351 into final per-liter components.

Composition

flowchart LR
    CultureMech_005590["desulfobacterium_anilini_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_005590 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005590 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_005590 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_005590 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005590 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_005590 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005590 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Resazurin["Resazurin"]
    CultureMech_005590 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_HCl["HCl"]
    CultureMech_005590 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_005590 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_005590 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005590 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_005590 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005590 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005590 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005590 --> ing15_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005590 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NaHCO3["NaHCO3"]
    CultureMech_005590 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_Phenol["Phenol"]
    CultureMech_005590 --> ing18_Phenol
    CHEBI_15882["CHEBI:15882"]
    ing18_Phenol -.-> CHEBI_15882
    ing19_Biotin["Biotin"]
    CultureMech_005590 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_005590 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005590 --> ing21_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_HCl -.-> CHEBI_30961
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005590 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Riboflavin["Riboflavin"]
    CultureMech_005590 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005590 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_005590 --> ing25_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Ca_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_005590 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005590 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_005590 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494
    ing29_Nicotinic_acid_amide["Nicotinic acid amide"]
    CultureMech_005590 --> ing29_Nicotinic_acid_amide
    CHEBI_17154["CHEBI:17154"]
    ing29_Nicotinic_acid_amide -.-> CHEBI_17154
    CultureMech_005590 --> more["...9 more"]

Ingredients (39)

#	Ingredient	Concentration	Ontology	Notes
1	Na2SO4 2Na.O4S 142.043 g/mol	2.991027 G_PER_L	CHEBI:32149 sodium sulfate	Archived DSMZ Medium 193 contributes 3.00 g Na2SO4 to a 1003 mL final formulation, yielding 2.991027 g/L.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.199402 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 193 contributes 0.20 g KH2PO4 to a 1003 mL final formulation, yielding 0.199402 g/L.
3	NH4Cl Cl.H4N 53.492 g/mol	0.299103 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 193 contributes 0.30 g NH4Cl to a 1003 mL final formulation, yielding 0.299103 g/L.
4	NaCl Cl.Na 58.443 g/mol	6.979063 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 193 contributes 7.00 g NaCl to a 1003 mL final formulation, yielding 6.979063 g/L.
5	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	1.296112 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 193 contributes 1.30 g MgCl2 x 6 H2O to a 1003 mL final formulation, yielding 1.296112 g/L.
6	KCl Cl.K 74.551 g/mol	0.498504 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 193 contributes 0.50 g KCl to a 1003 mL final formulation, yielding 0.498504 g/L.
7	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.149551 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 193 contributes 0.15 g CaCl2 x 2 H2O to a 1003 mL final formulation, yielding 0.149551 g/L.
8	Resazurin C12H7NO4 229.191 g/mol	0.000997 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 193 contributes 1.00 mg Resazurin to a 1003 mL final formulation, yielding 0.000997 g/L.
9	HCl HCl 36.461 g/mol	0.002493 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002493 g/L.
10	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001496 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001496 g/L.
11	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000698 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000698 g/L.
12	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000997 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000997 g/L.
13	H3BO3 H3BO3 61.833 g/mol	0.00000598 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000598 g/L.
14	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000189 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000189 g/L.
15	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000199 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000199 g/L.
16	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000239 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000239 g/L.
17	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000359 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1003 mL final formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000359 g/L.
18	NaHCO3 CHO3.Na 84.006 g/mol	4.985045 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 193 contributes 5.00 g NaHCO3 to a 1003 mL final formulation, yielding 4.985045 g/L.
19	Phenol C6H6O 94.113 g/mol	1.000 MILLIMOLAR	CHEBI:15882 phenol	Archived DSMZ Medium 476 replaces sodium acetate with 1 mM phenol.
20	Biotin C10H16N2O3S 244.316 g/mol	0.000120 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 351 Seven Vitamins Solution both contribute Biotin; their scaled contributions of 0.0000199 and 0.0000997 g/L yield 0.000120 g/L in the 1003 mL final formulation.
21	Folic acid C19H19N7O6 441.404 g/mol	0.0000199 G_PER_L	CHEBI:27470 folic acid	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.002 g/L Folic acid, yielding 0.0000199 g/L.
22	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.0000997 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.010 g/L Pyridoxine-HCl, yielding 0.0000997 g/L.
23	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000349 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 351 Seven Vitamins Solution both contribute Thiamine-HCl x 2 H2O; their scaled contributions of 0.0000499 and 0.000299 g/L yield 0.000349 g/L in the 1003 mL final formulation.
24	Riboflavin C17H20N4O6 376.369 g/mol	0.0000499 G_PER_L	CHEBI:17015 riboflavin	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.005 g/L Riboflavin, yielding 0.0000499 g/L.
25	Nicotinic acid C6H5NO2 123.111 g/mol	0.0000499 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.005 g/L Nicotinic acid, yielding 0.0000499 g/L.
26	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000499 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.005 g/L D-Ca-pantothenate, yielding 0.0000499 g/L.
27	Vitamin B12	0.0000508 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 351 Seven Vitamins Solution both contribute Vitamin B12; their scaled contributions of 0.000000997 and 0.0000499 g/L yield 0.0000508 g/L in the 1003 mL final formulation.
28	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000249 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 351 Seven Vitamins Solution both contribute p-Aminobenzoic acid; their scaled contributions of 0.0000499 and 0.000199 g/L yield 0.000249 g/L in the 1003 mL final formulation.
29	Lipoic acid C8H14O2S2 206.332 g/mol	0.0000499 G_PER_L	CHEBI:16494 lipoic acid	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1003 mL final formulation; the vitamin stock contains 0.005 g/L Lipoic acid, yielding 0.0000499 g/L.
30	Nicotinic acid amide C6H6N2O 122.127 g/mol	0.000349 G_PER_L	CHEBI:17154 nicotinamide	Archived DSMZ Medium 476 uses 1 mL/L of the Archived DSMZ Medium 351 Seven Vitamins Solution; the Seven Vitamins stock contains 0.350 g/L Nicotinic acid amide, yielding 0.000349 g/L in the 1003 mL final formulation.
31	Pyridoxal hydrochloride C8H9NO3.HCl 203.625 g/mol	0.0000997 G_PER_L	CHEBI:131529 pyridoxal hydrochloride	Archived DSMZ Medium 476 uses 1 mL/L of the Archived DSMZ Medium 351 Seven Vitamins Solution; the Seven Vitamins stock contains 0.100 g/L Pyridoxal hydrochloride, yielding 0.0000997 g/L in the 1003 mL final formulation.
32	Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000997 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 476 uses 1 mL/L of the Archived DSMZ Medium 351 Seven Vitamins Solution; the Seven Vitamins stock contains 0.100 g/L Ca-pantothenate, yielding 0.0000997 g/L in the 1003 mL final formulation.
33	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.398804 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 193 contributes 0.40 g Na2S x 9 H2O to a 1003 mL final formulation, yielding 0.398804 g/L.
34	NaOH HO.Na 39.997 g/mol	0.000499 G_PER_L	CHEBI:32145 sodium hydroxide	Archived DSMZ Medium 476 adds 1 mL/L of the Archived DSMZ Medium 385 selenite/tungsten solution; the stock contains 0.500 g/L NaOH, yielding 0.000499 g/L in the 1003 mL final formulation.
35	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.00000299 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 476 adds 1 mL/L of the Archived DSMZ Medium 385 selenite/tungsten solution; the stock contains 0.003 g/L Na2SeO3 x 5 H2O, yielding 0.00000299 g/L in the 1003 mL final formulation.
36	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.00000399 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Archived DSMZ Medium 476 adds 1 mL/L of the Archived DSMZ Medium 385 selenite/tungsten solution; the stock contains 0.004 g/L Na2WO4 x 2 H2O, yielding 0.00000399 g/L in the 1003 mL final formulation.
37	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 193 prepares the medium under 80% N2 and 20% CO2.
38	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 193 prepares the medium under 80% N2 and 20% CO2.
39	Distilled water H2O 18.015 g/mol	990.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 193 lists 990 mL direct distilled water across solutions A, C, D, and F before stock additions and before Archived DSMZ Medium 476 replaces the dissolved acetate with a phenol stock.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 476
2026-03-13T06:19:34.348129Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005590
2026-03-17T19:45:50.571295+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "DESULFOBACTERIUM ANILINI MEDIUM" to "desulfobacterium_anilini_medium" for consistent matching
2026-08-06T08:58:32.415297+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_476_score35.py	RESOLVED_KOMODO_476_SCORE35	Archived DSMZ Medium 476 defines DESULFOBACTERIUM ANILINI MEDIUM as DSMZ Medium 193 with 1 mM phenol replacing sodium acetate, with 1 mL/L DSMZ Medium 385 selenite/tungsten solution, and with 1 mL/L DSMZ Medium 351 Seven Vitamins Solution; this record expands archived DSMZ Media 193, 320, 141, 385, and 351 into final per-liter components.