for_thiobacillus_halophilus_dsm_6132

CultureMech:005606 bacterial DEFINED SOLID_AGAR
Original name: For Thiobacillus halophilus DSM 6132

Overview

External ontology
komodo.medium:484.1 (source link unavailable) — For Thiobacillus halophilus DSM 6132
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.6}
Notes
Source: KOMODO ModelSEED | ID: 484.1 | DSMZ Medium: 484 (mediadive.medium:484) | Aerobic: No

Composition

flowchart LR
    CultureMech_005606["for_thiobacillus_halophilus_dsm_6132"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_005606 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005606 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_005606 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005606 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_005606 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Agar["Agar"]
    CultureMech_005606 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_005606 --> ing6_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing6_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005606 --> ing7_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing8_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005606 --> ing8_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing8_Nicotinic_acid -.-> CHEBI_15940
    ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_005606 --> ing9_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing10_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005606 --> ing10_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing10_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing11_Riboflavin["Riboflavin"]
    CultureMech_005606 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_005606 --> ing12_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing12_Calcium_pantothenate -.-> CHEBI_31345
    ing13_Biotin["Biotin"]
    CultureMech_005606 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_005606 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_Na2_EDTA["Na2-EDTA"]
    CultureMech_005606 --> ing15_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing15_Na2_EDTA -.-> CHEBI_64734
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005606 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005606 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_005606 --> ing18__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing18__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005606 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_NaOH["NaOH"]
    CultureMech_005606 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
79 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
15 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.465116 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.116279 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NaCl
Cl.Na 58.443 g/mol
29.0698 G_PER_L CHEBI:26710
sodium chloride
6 Agar 17.4419 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
250 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
8 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
9 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
10 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
11 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.02 G_PER_L CHEBI:17015
riboflavin
13 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.02 G_PER_L CHEBI:31345
Calcium pantothenate
14 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
15 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
16 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
7.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.5 G_PER_L CHEBI:91249
ammonium molybdate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 NaOH
HO.Na 39.997 g/mol
11 G_PER_L CHEBI:32145
sodium hydroxide

Solutions

Trace element solution

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 11 G_PER_L
MnCl2 x 4 H2O 2.5 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 484.1
2026-02-03T11:19:18.667363 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 484
2026-03-13T06:19:34.528641Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005606
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:50.785109+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "For Thiobacillus halophilus DSM 6132" to "for_thiobacillus_halophilus_dsm_6132" for consistent matching
2026-05-20T06:01:20.391554+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.6' -> {'min': 7.3, 'max': 7.6}
2026-06-05T07:41:05.357003+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.765286+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-15T00:17:30.967748+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).