metallosphaera_medium (CultureMech:005608)

Overview

External ontology

komodo.medium:485 (source link unavailable) — METALLOSPHAERA medium

Applications

Microbial cultivation

Notes

KOMODO MediaInfo 485 lists 14 quantified g/L components for METALLOSPHAERA medium and also lists H2O and H2SO4 without gram or mol amounts.

Composition

flowchart LR
    CultureMech_005608["metallosphaera_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_005608 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_005608 --> ing1_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing1_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing2_sulfitic_ore__e_g__pyrite_["sulfitic ore (e.g. pyrite)"]
    CultureMech_005608 --> ing2_sulfitic_ore__e_g__pyrite_
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005608 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_005608 --> ing4_Yeast_extract
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005608 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005608 --> ing6_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing6_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005608 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005608 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_005608 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005608 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_H2O["H2O"]
    CultureMech_005608 --> ing11_H2O
    CHEBI_15377["CHEBI:15377"]
    ing11_H2O -.-> CHEBI_15377
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005608 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_005608 --> ing13_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing13_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing14_CoSO4["CoSO4"]
    CultureMech_005608 --> ing14_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing14_CoSO4 -.-> CHEBI_53470
    ing15_H2SO4["H2SO4"]
    CultureMech_005608 --> ing15_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing15_H2SO4 -.-> CHEBI_26836

Ingredients (16)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.28 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	KOMODO MediaInfo 485 lists 0.28 g/L KH2PO4.
2	VOSO4 x 2 H2O 2H2O.O4S.OV 199.036 g/mol	3.00E-5 G_PER_L	CHEBI:87009 vanadyl sulfate dihydrate	KOMODO MediaInfo 485 lists 3.00E-5 g/L VOSO4 x 2 H2O.
3	sulfitic ore (e.g. pyrite)	20.00 G_PER_L		KOMODO MediaInfo 485 lists 20.00 g/L sulfitic ore, e.g. pyrite.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.07 G_PER_L	CHEBI:86158 calcium chloride dihydrate	KOMODO MediaInfo 485 lists 0.07 g/L CaCl2 x 2 H2O.
5	Yeast extract	1.00 G_PER_L	FOODON:03315426 yeast extract	KOMODO MediaInfo 485 lists 1.00 g/L yeast extract.
6	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	1.80E-3 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	KOMODO MediaInfo 485 lists 1.80E-3 g/L MnCl2 x 4 H2O.
7	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	5.00E-5 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	KOMODO MediaInfo 485 lists 5.00E-5 g/L CuCl2 x 2 H2O.
8	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.30 G_PER_L	CHEBI:62946 ammonium sulfate	KOMODO MediaInfo 485 lists 1.30 g/L (NH4)2SO4.
9	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3.00E-5 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	KOMODO MediaInfo 485 lists 3.00E-5 g/L Na2MoO4 x 2 H2O.
10	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.02 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	KOMODO MediaInfo 485 lists 0.02 g/L FeCl3 x 6 H2O.
11	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	KOMODO MediaInfo 485 lists 0.25 g/L MgSO4 x 7 H2O.
12	H2O H2O 18.015 g/mol		CHEBI:15377 water	KOMODO MediaInfo 485 lists H2O without a gram or mol amount.
13	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	2.20E-4 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	KOMODO MediaInfo 485 lists 2.20E-4 g/L ZnSO4 x 7 H2O.
14	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	4.50E-3 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	KOMODO MediaInfo 485 lists 4.50E-3 g/L Na2B4O7 x 10 H2O.
15	CoSO4 Co.O4S 154.996 g/mol	1.00E-5 G_PER_L	CHEBI:53470 cobalt(2+) sulfate	KOMODO MediaInfo 485 lists 1.00E-5 g/L CoSO4.
16	H2SO4 H2O4S 98.079 g/mol		CHEBI:26836 sulfuric acid	KOMODO MediaInfo 485 lists H2SO4 without a gram or mol amount.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 485
2026-03-13T06:19:34.543958Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005608
2026-03-17T19:45:50.803008+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METALLOSPHAERA medium" to "metallosphaera_medium" for consistent matching
2026-08-06T08:58:32.363922+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_485_score35.py	RESOLVED_KOMODO_485_SCORE35	KOMODO MediaInfo 485 lists 14 quantified g/L components for METALLOSPHAERA medium and also lists H2O and H2SO4 without gram or mol amounts.