desulfovibrio_alcoholovorans_medium (CultureMech:005613)

Overview

External ontology

komodo.medium:488 (source link unavailable) — DESULFOVIBRIO ALCOHOLOVORANS medium

Applications

Microbial cultivation

pH

7.8

Notes

Archived DSMZ Medium 488 defines DESULFOVIBRIO ALCOHOLOVORANS MEDIUM as DSMZ Medium 63 or 194 with 1.5 g/L 1,2-propanediol as substrate and 3 micrograms/L Na2SeO3 x 5 H2O; KOMODO Medium 488 expands the DSMZ Medium 63 branch, so this record expands archived DSMZ Media 488 and 63 into final per-liter components.

Composition

flowchart LR
    CultureMech_005613["desulfovibrio_alcoholovorans_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_005613 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_005613 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_005613 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005613 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005613 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_DL_Na_lactate["DL-Na-lactate"]
    CultureMech_005613 --> ing5_DL_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_DL_Na_lactate -.-> CHEBI_75228
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_005613 --> ing6_Yeast_extract
    ing7_Resazurin["Resazurin"]
    CultureMech_005613 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005613 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_005613 --> ing9_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing9_Na_thioglycolate -.-> CHEBI_86481
    ing10_Ascorbic_acid["Ascorbic acid"]
    CultureMech_005613 --> ing10_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing10_Ascorbic_acid -.-> CHEBI_22652
    ing11_1_2_propanediol["1,2-propanediol"]
    CultureMech_005613 --> ing11_1_2_propanediol
    CHEBI_16997["CHEBI:16997"]
    ing11_1_2_propanediol -.-> CHEBI_16997
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005613 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_NaOH["NaOH"]
    CultureMech_005613 --> ing13_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing13_NaOH -.-> CHEBI_32145
    ing14_N2["N2"]
    CultureMech_005613 --> ing14_N2
    CHEBI_17997["CHEBI:17997"]
    ing14_N2 -.-> CHEBI_17997
    ing15_Distilled_water["Distilled water"]
    CultureMech_005613 --> ing15_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing15_Distilled_water -.-> CHEBI_15377

Ingredients (16)

#	Ingredient	Concentration	Ontology	Notes
1	K2HPO4 HO4P.2K 174.174 g/mol	0.50 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 63 lists 0.50 g K2HPO4 in the final 1000 mL medium.
2	NH4Cl Cl.H4N 53.492 g/mol	1.00 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 63 lists 1.00 g NH4Cl in the final 1000 mL medium.
3	Na2SO4 2Na.O4S 142.043 g/mol	1.00 G_PER_L	CHEBI:32149 sodium sulfate	Archived DSMZ Medium 63 lists 1.00 g Na2SO4 in the final 1000 mL medium.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.10 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 63 lists 0.10 g CaCl2 x 2 H2O in the final 1000 mL medium.
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	2.00 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 63 lists 2.00 g MgSO4 x 7 H2O in the final 1000 mL medium.
6	DL-Na-lactate C3H5O3.Na 112.06 g/mol	2.00 G_PER_L	CHEBI:75228 sodium lactate	Archived DSMZ Medium 63 lists 2.00 g DL-Na-lactate in the final 1000 mL medium.
7	Yeast extract	1.00 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 63 lists 1.00 g Yeast extract in the final 1000 mL medium.
8	Resazurin C12H7NO4 229.191 g/mol	0.001000 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 63 lists 1.00 mg Resazurin in the final 1000 mL medium.
9	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.50 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 63 lists 0.50 g FeSO4 x 7 H2O in the final 1000 mL medium.
10	Na-thioglycolate C2H3O2S.Na 114.101 g/mol	0.10 G_PER_L	CHEBI:86481 sodium thioglycolate	Archived DSMZ Medium 63 lists 0.10 g Na-thioglycolate in the final 1000 mL medium.
11	Ascorbic acid	0.10 G_PER_L	CHEBI:22652 ascorbic acid	Archived DSMZ Medium 63 lists 0.10 g Ascorbic acid in the final 1000 mL medium.
12	1,2-propanediol C3H8O2 76.095 g/mol	1.50 G_PER_L	CHEBI:16997 propane-1,2-diol	Archived DSMZ Medium 488 adds 1.5 g/L 1,2-propanediol as the substrate.
13	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	3.0 MICROG_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 488 adds 3 micrograms/L Na2SeO3 x 5 H2O.
14	NaOH HO.Na 39.997 g/mol	variable VARIABLE	CHEBI:32145 sodium hydroxide	Archived DSMZ Medium 63 adjusts the final pH to 7.8 with NaOH.
15	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 63 gasses the medium with oxygen-free N2.
16	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 63 combines 980 mL distilled water in Solution A, 10 mL in Solution B, and 10 mL in Solution C.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 488
2026-03-13T06:19:34.588353Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005613
2026-03-17T19:45:50.852257+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "DESULFOVIBRIO ALCOHOLOVORANS medium" to "desulfovibrio_alcoholovorans_medium" for consistent matching
2026-08-06T08:58:32.422270+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_488_score35.py	RESOLVED_KOMODO_488_SCORE35	Archived DSMZ Medium 488 defines DESULFOVIBRIO ALCOHOLOVORANS MEDIUM as DSMZ Medium 63 or 194 with 1.5 g/L 1,2-propanediol as substrate and 3 micrograms/L Na2SeO3 x 5 H2O; KOMODO Medium 488 expands the DSMZ Medium 63 branch, so this record expands archived DSMZ Media 488 and 63 into final per-liter components.