Overview
- External ontology
- komodo.medium:489 (source link unavailable) — CLOSTRIDIUM AEROTOLERANS medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 489 | DSMZ Medium: 489 (mediadive.medium:489) | Aerobic: Yes Preparation scope: cysteine and sulfide are added after sterilization from sterile anoxic stocks prepared under 100% N2. Their 0.15 g batch inputs do not establish stock strengths or addition volumes. Resazurin is a 0.1% w/v stock added at 0.50 ml per batch. Water is a 1000 ml input; final-medium volume and final concentrations are not inferred. Historical annotations, including any aerobic flag, are not verified incubation or growth evidence; the source establishes anoxic preparation. Chemical mappings and variant links are retained, not independently revalidated.
Composition
flowchart LR
CultureMech_005614["clostridium_aerotolerans_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_005614 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_005614 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_005614 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_005614 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005614 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005614 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Yeast_extract["Yeast extract"]
CultureMech_005614 --> ing6_Yeast_extract
ing7_Xylan["Xylan"]
CultureMech_005614 --> ing7_Xylan
CHEBI_37166["CHEBI:37166"]
ing7_Xylan -.-> CHEBI_37166
ing8_Distilled_water["Distilled water"]
CultureMech_005614 --> ing8_Distilled_water
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.22 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 0.22 g. Retained 0.22 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.22 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 0.22 g. Retained 0.22 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.45 G_PER_L |
CHEBI:26710
sodium chloride
|
Source batch input: 0.45 g. Retained 0.45 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Source batch input: 0.45 g. Retained 0.45 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Source batch input: 0.06 g. Retained 0.06 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.09 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 0.09 g. Retained 0.09 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 7 | Yeast extract | 5 G_PER_L | Source batch input: 5 g. Retained 5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. | |
| 8 | Xylan | 5 G_PER_L |
CHEBI:37166
xylan
|
Source batch input: 5 g. Retained 5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 9 | Distilled water | 1 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
1000 ml distilled-water input per source batch, not a volume percentage or measured final medium volume. |
Preparation
- Dissolve ingredients (except cysteine and sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas.
Solutions
Sodium resazurin stock (0.1% w/v)
Dose: 0.0005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium resazurin | 0.1 PERCENT_W_V | Source-explicit 0.1% w/v stock strength. The former 0.0005 g/L recipe rate is not the stock concentration. Existing resazurin ontology mapping is retained, not independently validated as the sodium salt. |
0.50 ml stock input per source recipe batch, not per liter of measured final medium. No solvent identity is inferred.
L-Cysteine HCl x H2O sterile anoxic stock
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl x H2O | Source batch requirement: 0.15 g supplied from a sterile anoxic stock prepared under 100% N2, added after sterilization. Stock strength, final stock volume, solvent identity and numeric stock dose are unspecified; the former 0.15 g/L flattened rate is withdrawn at this preparation stage. |
Source batch requirement: 0.15 g supplied from a sterile anoxic stock prepared under 100% N2, added after sterilization. Stock strength, final stock volume, solvent identity and numeric stock dose are unspecified; the former 0.15 g/L flattened rate is withdrawn at this preparation stage.
Na2S x 9 H2O sterile anoxic stock
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9 H2O | Source batch requirement: 0.15 g supplied from a sterile anoxic stock prepared under 100% N2, added after sterilization. Stock strength, final stock volume, solvent identity and numeric stock dose are unspecified; the former 0.15 g/L flattened rate is withdrawn at this preparation stage. |
Source batch requirement: 0.15 g supplied from a sterile anoxic stock prepared under 100% N2, added after sterilization. Stock strength, final stock volume, solvent identity and numeric stock dose are unspecified; the former 0.15 g/L flattened rate is withdrawn at this preparation stage.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 489 |
| 2026-02-03T11:18:58.550985 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 489 |
| 2026-03-13T06:19:34.592448Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005614 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:50.856972+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM AEROTOLERANS medium" to "clostridium_aerotolerans_medium" for consistent matching |
| 2026-06-05T07:40:24.053348+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.134042+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T01:10:18.404748+00:00 | codex | CORRECTED_DSMZ489_STOCK_BOUNDARIES_AND_WATER_INPUT | Restored explicit resazurin stock strength/dose and separate post-sterilization reducing stocks. Corrected/restored water input. Preserved history, exact previous snippets, mappings and variants; no unknown stock strength, solvent, final volume or growth association inferred. |