Overview
- External ontology
- komodo.medium:491 (source link unavailable) — HYPERTHERMUS BUTYLICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.5, 'max': 7.0}
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 491 | DSMZ Medium: 491 (mediadive.medium:491) | Aerobic: Yes
Composition
flowchart LR
CultureMech_005619["hyperthermus_butylicus_medium"]
ing0_NaCl["NaCl"]
CultureMech_005619 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005619 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005619 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_005619 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaBr["NaBr"]
CultureMech_005619 --> ing4_NaBr
CHEBI_63004["CHEBI:63004"]
ing4_NaBr -.-> CHEBI_63004
ing5_H3BO3["H3BO3"]
CultureMech_005619 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_005619 --> ing6_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_005619 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_NH4Cl["NH4Cl"]
CultureMech_005619 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
ing9_Citric_acid["Citric acid"]
CultureMech_005619 --> ing9_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing9_Citric_acid -.-> CHEBI_30769
ing10_KI["KI"]
CultureMech_005619 --> ing10_KI
CHEBI_8346["CHEBI:8346"]
ing10_KI -.-> CHEBI_8346
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005619 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_KH2PO4["KH2PO4"]
CultureMech_005619 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005619 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Tryptone["Tryptone"]
CultureMech_005619 --> ing14_Tryptone
ing15_Sodium_resazurin["Sodium resazurin"]
CultureMech_005619 --> ing15_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing15_Sodium_resazurin -.-> CHEBI_8806
ing16_Sulfur["Sulfur"]
CultureMech_005619 --> ing16_Sulfur
CHEBI_26833["CHEBI:26833"]
ing16_Sulfur -.-> CHEBI_26833
ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005619 --> ing17_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing17_Na2S_x_9_H2O -.-> CHEBI_76209
ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_005619 --> ing18_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
ing19_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_005619 --> ing19_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing19_MnSO4_x_H2O -.-> CHEBI_86364
ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005619 --> ing20_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_005619 --> ing21_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005619 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_005619 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_005619 --> ing24_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing24_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005619 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_005619 --> ing26_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_005619 --> ing27_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing28_H2SO4["H2SO4"]
CultureMech_005619 --> ing28_H2SO4
CHEBI_26836["CHEBI:26836"]
ing28_H2SO4 -.-> CHEBI_26836
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
17.5531 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 16.5531, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.40798 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.40798, 3.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.6777 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.321324 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaBr
Br.Na 102.894 g/mol
|
0.0486855 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
0.0246056 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.0146056, 0.01] |
| 7 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00681597 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.0097371 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.486855 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Citric acid
C6H8O7 192.123 g/mol
|
0.00486855 G_PER_L |
CHEBI:30769
citric acid
|
|
| 11 |
KI
I.K 166.002 g/mol
|
0.00243427 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.830282 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.730282, 0.1] |
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.486855 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03194742 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00194742, 0.03] |
| 15 | Tryptone | 5.84226 G_PER_L | ||
| 16 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000486855 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 17 |
Sulfur
S 32.067 g/mol
|
5.84226 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 18 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.292113 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 19 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 20 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 21 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 22 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 25 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 28 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 29 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 491 |
| 2026-02-03T11:19:00.630081 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 491 |
| 2026-03-13T03:08:53.430043Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s); Added pH buffer: H2SO4 |
| 2026-03-13T06:19:34.634383Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005619 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:50.904950+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPERTHERMUS BUTYLICUS MEDIUM" to "hyperthermus_butylicus_medium" for consistent matching |
| 2026-05-16T09:03:03.158380+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:00:52.282351+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0} |
| 2026-06-05T07:40:24.081621+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.140614+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |