thermotoga_ii_medium

CultureMech:005631 bacterial COMPLEX LIQUID
Original name: THERMOTOGA II medium

Overview

External ontology
komodo.medium:498 (source link unavailable) — THERMOTOGA II medium
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: KOMODO ModelSEED | ID: 498 | DSMZ Medium: 498 (mediadive.medium:498) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_005631["thermotoga_ii_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_005631 --> ing0_Yeast_extract
    ing1_NaCl["NaCl"]
    CultureMech_005631 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Na2_EDTA["Na2-EDTA"]
    CultureMech_005631 --> ing2_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing2_Na2_EDTA -.-> CHEBI_64734
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005631 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005631 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005631 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_005631 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaBr["NaBr"]
    CultureMech_005631 --> ing7_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing7_NaBr -.-> CHEBI_63004
    ing8_H3BO3["H3BO3"]
    CultureMech_005631 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
    CultureMech_005631 --> ing9__NH4_2Ni_SO4_2
    CHEBI_86149["CHEBI:86149"]
    ing9__NH4_2Ni_SO4_2 -.-> CHEBI_86149
    ing10_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_005631 --> ing10_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing10_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing11_KI["KI"]
    CultureMech_005631 --> ing11_KI
    CHEBI_8346["CHEBI:8346"]
    ing11_KI -.-> CHEBI_8346
    ing12_Sodium_resazurin["Sodium resazurin"]
    CultureMech_005631 --> ing12_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Sodium_resazurin -.-> CHEBI_8806
    ing13_KH2PO4["KH2PO4"]
    CultureMech_005631 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_Starch["Starch"]
    CultureMech_005631 --> ing14_Starch
    CHEBI_28017["CHEBI:28017"]
    ing14_Starch -.-> CHEBI_28017
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005631 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_005631 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_005631 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005631 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_005631 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005631 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_005631 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_005631 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005631 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005631 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005631 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_005631 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.488281 G_PER_L
2 NaCl
Cl.Na 58.443 g/mol
4.378909999999999 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 3.37891, 1.0]
3 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.751953 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.859375 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.859375, 3.0]
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.673828 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.236719 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.136719, 0.1]
7 KCl
Cl.K 74.551 g/mol
0.078125 G_PER_L CHEBI:32588
potassium chloride
8 NaBr
Br.Na 102.894 g/mol
0.012207 G_PER_L CHEBI:63004
sodium bromide
9 H3BO3
H3BO3 61.833 g/mol
0.01390625 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.00390625, 0.01]
10 (NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.00292969 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
11 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00185547 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
12 KI
I.K 166.002 g/mol
9.76562e-06 G_PER_L CHEBI:8346
potassium iodide
13 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000488281 G_PER_L CHEBI:8806
Resazurin
14 KH2PO4
H2O4P.K 136.084 g/mol
0.488281 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
15 Starch 4.88281 G_PER_L CHEBI:28017
starch
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.488281 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 498
2026-02-03T11:19:17.611616 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 498
2026-03-13T03:09:38.621436Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:34.783194Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005631
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:51.074593+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOTOGA II medium" to "thermotoga_ii_medium" for consistent matching
2026-06-05T07:41:50.744736+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:13.241670+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.