stygiolobus_medium (CultureMech:005712)

Overview

External ontology

komodo.medium:510 (source link unavailable) — STYGIOLOBUS medium

Applications

Microbial cultivation

pH range

{'min': 2.5, 'max': 3.0}

Notes

Archived DSMZ Medium 510 defines STYGIOLOBUS MEDIUM as the anaerobic branch of DSMZ Medium 358, inheriting the DSMZ Medium 88 salt base, with final 0.2 g/L yeast extract, 5 g/L sulfur, 0.5 mg/L resazurin, pH 2.5-3.0, and 200 kPa 80:20 H2/CO2 overpressure.

Composition

flowchart LR
    CultureMech_005712["stygiolobus_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005712 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005712 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005712 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005712 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_005712 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005712 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_005712 --> ing6_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing6_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_005712 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005712 --> ing8_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing8_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005712 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_005712 --> ing10_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing10_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing11_CoSO4["CoSO4"]
    CultureMech_005712 --> ing11_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing11_CoSO4 -.-> CHEBI_53470
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_005712 --> ing12_Yeast_extract
    ing13_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_005712 --> ing13_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing13_Sulfur__powder_ -.-> CHEBI_33403
    ing14_Resazurin["Resazurin"]
    CultureMech_005712 --> ing14_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Resazurin -.-> CHEBI_8806
    ing15_H2SO4["H2SO4"]
    CultureMech_005712 --> ing15_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing15_H2SO4 -.-> CHEBI_26836
    ing16_H2["H2"]
    CultureMech_005712 --> ing16_H2
    CHEBI_18276["CHEBI:18276"]
    ing16_H2 -.-> CHEBI_18276
    ing17_CO2["CO2"]
    CultureMech_005712 --> ing17_CO2
    CHEBI_16526["CHEBI:16526"]
    ing17_CO2 -.-> CHEBI_16526
    ing18_Demineralized_water["Demineralized water"]
    CultureMech_005712 --> ing18_Demineralized_water
    CHEBI_15377["CHEBI:15377"]
    ing18_Demineralized_water -.-> CHEBI_15377

Ingredients (19)

#	Ingredient	Concentration	Ontology	Notes
1	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.300000 G_PER_L	CHEBI:62946 ammonium sulfate	Archived DSMZ Medium 88 lists 1.30 g/L (NH4)2SO4 per liter in the salt base.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.280000 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 88 lists 0.28 g/L KH2PO4 per liter in the salt base.
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.250000 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 88 lists 0.25 g/L MgSO4 x 7 H2O per liter in the salt base.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.070000 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 88 lists 0.07 g/L CaCl2 x 2 H2O per liter in the salt base.
5	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.020000 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	Archived DSMZ Medium 88 lists 0.02 g/L FeCl3 x 6 H2O per liter in the salt base.
6	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.001800 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 88 lists 1.80 mg/L MnCl2 x 4 H2O per liter in the salt base.
7	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	0.004500 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	Archived DSMZ Medium 88 lists 4.50 mg/L Na2B4O7 x 10 H2O per liter in the salt base.
8	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.000220 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 88 lists 0.22 mg/L ZnSO4 x 7 H2O per liter in the salt base.
9	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.000050 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 88 lists 0.05 mg/L CuCl2 x 2 H2O per liter in the salt base.
10	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000030 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 88 lists 0.03 mg/L Na2MoO4 x 2 H2O per liter in the salt base.
11	VOSO4 x 2 H2O 2H2O.O4S.OV 199.036 g/mol	0.000030 G_PER_L	CHEBI:87009 vanadyl sulfate dihydrate	Archived DSMZ Medium 88 lists 0.03 mg/L VOSO4 x 2 H2O per liter in the salt base.
12	CoSO4 Co.O4S 154.996 g/mol	0.000010 G_PER_L	CHEBI:53470 cobalt(II) sulfate	Archived DSMZ Medium 88 lists 0.01 mg/L CoSO4 per liter in the salt base.
13	Yeast extract	0.200000 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 510 sets the final STYGIOLOBUS medium to 0.2 g/L Yeast extract.
14	Sulfur (powder)	5.000000 G_PER_L	CHEBI:33403 elemental sulfur	Archived DSMZ Medium 510 sets the final STYGIOLOBUS medium to 5 g/L Sulfur (powder).
15	Resazurin C12H7NO4 229.191 g/mol	0.000500 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 510 sets the final STYGIOLOBUS medium to 0.5 mg/L Resazurin.
16	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	Archived DSMZ Medium 510 adjusts the final medium to pH 2.5-3.0.
17	H2 H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Archived DSMZ Medium 510 pressurizes inoculated bottles with 80% H2 and 20% CO2.
18	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 510 pressurizes inoculated bottles with 80% H2 and 20% CO2.
19	Demineralized water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 88 lists 1000 mL demineralized water per liter.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 510
2026-03-13T06:19:35.662185Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005712
2026-03-17T19:45:52.102128+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "STYGIOLOBUS medium" to "stygiolobus_medium" for consistent matching
2026-05-20T06:00:52.388710+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '2.5-3.0' -> {'min': 2.5, 'max': 3.0}
2026-08-06T08:58:32.361497+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_510_score30.py	RESOLVED_KOMODO_510_SCORE30	Archived DSMZ Medium 510 defines STYGIOLOBUS MEDIUM as the anaerobic branch of DSMZ Medium 358, inheriting the DSMZ Medium 88 salt base, with final 0.2 g/L yeast extract, 5 g/L sulfur, 0.5 mg/L resazurin, pH 2.5-3.0, and 200 kPa 80:20 H2/CO2 overpressure.