Overview
- External ontology
- komodo.medium:514b.2 (source link unavailable) — For strains DSM 5349, DSM 24106, DSM 24107, DSM 24186, DSM 23100, DSM 21021, DSM 19800, DSM 19821
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: KOMODO ModelSEED | ID: 514b.2 | DSMZ Medium: 514b (mediadive.medium:514b) | Aerobic: No
Composition
flowchart LR
CultureMech_005859["for_strains_dsm_5349_dsm_24106_dsm_24107_dsm_24186_dsm_23100_dsm_21..."]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_005859 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_005859 --> ing1_Yeast_extract
ing2_Fe_III__citrate["Fe(III) citrate"]
CultureMech_005859 --> ing2_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing2_Fe_III__citrate -.-> CHEBI_144421
ing3_NaCl["NaCl"]
CultureMech_005859 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2["MgCl2"]
CultureMech_005859 --> ing4_MgCl2
CHEBI_6636["CHEBI:6636"]
ing4_MgCl2 -.-> CHEBI_6636
ing5_Na2SO4["Na2SO4"]
CultureMech_005859 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_CaCl2["CaCl2"]
CultureMech_005859 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_KCl["KCl"]
CultureMech_005859 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_005859 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_KBr["KBr"]
CultureMech_005859 --> ing9_KBr
CHEBI_32030["CHEBI:32030"]
ing9_KBr -.-> CHEBI_32030
ing10_SrCl2["SrCl2"]
CultureMech_005859 --> ing10_SrCl2
CHEBI_36383["CHEBI:36383"]
ing10_SrCl2 -.-> CHEBI_36383
ing11_H3BO3["H3BO3"]
CultureMech_005859 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_Na_silicate["Na-silicate"]
CultureMech_005859 --> ing12_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing12_Na_silicate -.-> CHEBI_60720
ing13_NaF["NaF"]
CultureMech_005859 --> ing13_NaF
CHEBI_28741["CHEBI:28741"]
ing13_NaF -.-> CHEBI_28741
ing14__NH4_NO3["(NH4)NO3"]
CultureMech_005859 --> ing14__NH4_NO3
CHEBI_63038["CHEBI:63038"]
ing14__NH4_NO3 -.-> CHEBI_63038
ing15_Na2HPO4["Na2HPO4"]
CultureMech_005859 --> ing15_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing15_Na2HPO4 -.-> CHEBI_34683
ing16_Sea_water["Sea water"]
CultureMech_005859 --> ing16_Sea_water
ing17_Agar["Agar"]
CultureMech_005859 --> ing17_Agar
CHEBI_2509["CHEBI:2509"]
ing17_Agar -.-> CHEBI_2509
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 2.5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.05 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
9.725 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2
2Cl.Mg 95.211 g/mol
|
2.95 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.62 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.9 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.275 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.08 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
KBr
Br.K 119.002 g/mol
|
0.04 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 11 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.017 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.011 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
0.002 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 14 |
NaF
F.Na 41.988 g/mol
|
0.0012 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 15 |
(NH4)NO3
H4N.NO3 80.043 g/mol
|
0.0008 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 16 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.004 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 17 | Sea water | 500 G_PER_L | ||
| 18 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 514b.2 |
| 2026-02-03T11:19:19.124940 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 514 |
| 2026-03-13T06:19:36.836822Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005859 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:53.447881+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For strains DSM 5349, DSM 24106, DSM 24107, DSM 24186, DSM 23100, DSM 21021, DSM 19800, DSM 19821" to "for_strains_dsm_5349_dsm_24106_dsm_24107_dsm_24186_dsm_23100_dsm_21021_dsm_19800_dsm_19821" for consistent matching |
| 2026-06-05T07:41:05.305659+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.747472+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |