acetonema_medium (CultureMech:005871)

Overview

External ontology

komodo.medium:515 (source link unavailable) — ACETONEMA medium

Applications

Microbial cultivation

pH

7.0

Notes

Archived DSMZ Medium 515 defines ACETONEMA MEDIUM as DSMZ Medium 311 with 0.2% separately sterilized glucose replacing betaine and 1 mM dithiothreitol replacing cysteine and sulfide; this record expands archived DSMZ Media 311, 320, and 141 into final per-liter components.

Composition

flowchart LR
    CultureMech_005871["acetonema_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_005871 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_005871 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_005871 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005871 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005871 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_005871 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005871 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_HCl["HCl"]
    CultureMech_005871 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_005871 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_005871 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005871 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_005871 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005871 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005871 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005871 --> ing14_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005871 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaHSeO3["NaHSeO3"]
    CultureMech_005871 --> ing16_NaHSeO3
    CHEBI_29924["CHEBI:29924"]
    ing16_NaHSeO3 -.-> CHEBI_29924
    ing17_Yeast_extract["Yeast extract"]
    CultureMech_005871 --> ing17_Yeast_extract
    ing18_Casitone["Casitone"]
    CultureMech_005871 --> ing18_Casitone
    ing19_D_Glucose["D-Glucose"]
    CultureMech_005871 --> ing19_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing19_D_Glucose -.-> CHEBI_17634
    ing20_NaHCO3["NaHCO3"]
    CultureMech_005871 --> ing20_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing20_NaHCO3 -.-> CHEBI_32139
    ing21_Resazurin["Resazurin"]
    CultureMech_005871 --> ing21_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing21_Resazurin -.-> CHEBI_8806
    ing22_Dithiothreitol["Dithiothreitol"]
    CultureMech_005871 --> ing22_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing22_Dithiothreitol -.-> CHEBI_18320
    ing23_Biotin["Biotin"]
    CultureMech_005871 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_005871 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005871 --> ing25_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_HCl -.-> CHEBI_30961
    ing26_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005871 --> ing26_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing26_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing27_Riboflavin["Riboflavin"]
    CultureMech_005871 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005871 --> ing28_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing28_Nicotinic_acid -.-> CHEBI_15940
    ing29_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_005871 --> ing29_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_D_Ca_pantothenate -.-> CHEBI_31345
    CultureMech_005871 --> more["...6 more"]

Ingredients (36)

#	Ingredient	Concentration	Ontology	Notes
1	K2HPO4 HO4P.2K 174.174 g/mol	0.344214 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 311 contributes 0.348 g K2HPO4 to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.344214 g/L in 1011 mL final formulation.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.224530 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 311 contributes 0.227 g KH2PO4 to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.224530 g/L in 1011 mL final formulation.
3	NH4Cl Cl.H4N 53.492 g/mol	0.494560 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 311 contributes 0.500 g NH4Cl to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.494560 g/L in 1011 mL final formulation.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.494560 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 311 contributes 0.500 g MgSO4 x 7 H2O to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.494560 g/L in 1011 mL final formulation.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.247280 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 311 contributes 0.250 g CaCl2 x 2 H2O to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.247280 g/L in 1011 mL final formulation.
6	NaCl Cl.Na 58.443 g/mol	2.225519 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 311 contributes 2.250 g NaCl to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 2.225519 g/L in 1011 mL final formulation.
7	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.001978 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 311 contributes 0.002 g FeSO4 x 7 H2O to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.001978 g/L in 1011 mL final formulation.
8	HCl HCl 36.461 g/mol	0.002473 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002473 g/L.
9	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001484 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001484 g/L.
10	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000692 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000692 g/L.
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000989 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000989 g/L.
12	H3BO3 H3BO3 61.833 g/mol	0.00000593 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000593 g/L.
13	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000188 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000188 g/L.
14	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000198 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000198 g/L.
15	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000237 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000237 g/L.
16	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000356 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 311 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution per 1011 mL final formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000356 g/L.
17	NaHSeO3 HO3Se 127.965 g/mol	0.100 MICROMOLAR	CHEBI:29924 hydrogenselenite	Archived DSMZ Medium 311 specifies NaHSeO3 at a final 10^-7 M concentration.
18	Yeast extract	1.978239 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 311 contributes 2.000 g Yeast extract to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 1.978239 g/L in 1011 mL final formulation.
19	Casitone	1.978239 G_PER_L		Archived DSMZ Medium 311 contributes 2.000 g Casitone to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 1.978239 g/L in 1011 mL final formulation.
20	D-Glucose C6H12O6 180.156 g/mol	2.000000 G_PER_L	CHEBI:17634 D-glucose	Archived DSMZ Medium 515 replaces betaine with 0.2% separately sterilized glucose, equivalent to 2 g/L.
21	NaHCO3 CHO3.Na 84.006 g/mol	3.956479 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 311 contributes 4.000 g NaHCO3 to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 3.956479 g/L in 1011 mL final formulation.
22	Resazurin C12H7NO4 229.191 g/mol	0.000989 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 311 contributes 1.000 mg Resazurin to 1000 mL distilled water plus 10 mL vitamin stock and 1 mL SL-10 trace stock, yielding 0.000989 g/L in 1011 mL final formulation.
23	Dithiothreitol C4H10O2S2 154.256 g/mol	1.000 MILLIMOLAR	CHEBI:18320 1,4-dithiothreitol	Archived DSMZ Medium 515 replaces cysteine and sodium sulfide with 1 mmol/L dithiothreitol.
24	Biotin C10H16N2O3S 244.316 g/mol	0.0000198 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.002 g/L Biotin, yielding 0.0000198 g/L.
25	Folic acid C19H19N7O6 441.404 g/mol	0.0000198 G_PER_L	CHEBI:27470 folic acid	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.002 g/L Folic acid, yielding 0.0000198 g/L.
26	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.0000989 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.010 g/L Pyridoxine-HCl, yielding 0.0000989 g/L.
27	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.0000495 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L Thiamine-HCl x 2 H2O, yielding 0.0000495 g/L.
28	Riboflavin C17H20N4O6 376.369 g/mol	0.0000495 G_PER_L	CHEBI:17015 riboflavin	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L Riboflavin, yielding 0.0000495 g/L.
29	Nicotinic acid C6H5NO2 123.111 g/mol	0.0000495 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L Nicotinic acid, yielding 0.0000495 g/L.
30	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000495 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L D-Ca-pantothenate, yielding 0.0000495 g/L.
31	Vitamin B12	0.000000989 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.0001 g/L Vitamin B12, yielding 0.000000989 g/L.
32	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.0000495 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L p-Aminobenzoic acid, yielding 0.0000495 g/L.
33	Lipoic acid C8H14O2S2 206.332 g/mol	0.0000495 G_PER_L	CHEBI:16494 lipoic acid	Archived DSMZ Medium 311 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution per 1011 mL final formulation; the vitamin stock contains 0.005 g/L Lipoic acid, yielding 0.0000495 g/L.
34	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 311 prepares the medium under 80% N2 and 20% CO2.
35	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 311 prepares the medium under 80% N2 and 20% CO2.
36	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 311 lists 1000 mL distilled water before addition of 10 mL vitamin stock and 1 mL SL-10 trace stock.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 515
2026-03-13T06:19:36.927447Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005871
2026-03-17T19:45:53.553350+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ACETONEMA medium" to "acetonema_medium" for consistent matching
2026-08-06T08:58:32.383827+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_515_score35.py	RESOLVED_KOMODO_515_SCORE35	Archived DSMZ Medium 515 defines ACETONEMA MEDIUM as DSMZ Medium 311 with 0.2% separately sterilized glucose replacing betaine and 1 mM dithiothreitol replacing cysteine and sulfide; this record expands archived DSMZ Media 311, 320, and 141 into final per-liter components.