clostridium_neopropionicum_medium (CultureMech:005884)

Overview

External ontology

komodo.medium:522 (source link unavailable) — CLOSTRIDIUM NEOPROPIONICUM medium

Applications

Microbial cultivation

pH

7.0

Notes

DSMZ Medium 522 defines CLOSTRIDIUM NEOPROPIONICUM MEDIUM as DSMZ Medium 318 with 4.0 g/L KHCO3 for pH 7.0 and 1.0 g/L ethanol replacing methanol; this record expands the DSMZ Medium 318 trace element stock and the DSMZ Medium 141 vitamin stock into final per-liter components.

Composition

flowchart LR
    CultureMech_005884["clostridium_neopropionicum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_005884 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaCl["NaCl"]
    CultureMech_005884 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_005884 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005884 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_005884 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_005884 --> ing5_Yeast_extract
    ing6_Trypticase["Trypticase"]
    CultureMech_005884 --> ing6_Trypticase
    ing7_Resazurin["Resazurin"]
    CultureMech_005884 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_KHCO3["KHCO3"]
    CultureMech_005884 --> ing8_KHCO3
    CHEBI_81862["CHEBI:81862"]
    ing8_KHCO3 -.-> CHEBI_81862
    ing9_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_005884 --> ing9_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005884 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
    CultureMech_005884 --> ing11_Nitrilotriacetic_acid__NTA_
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
    ing12_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_005884 --> ing12_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing12_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005884 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005884 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_ZnCl2["ZnCl2"]
    CultureMech_005884 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005884 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_H3BO3["H3BO3"]
    CultureMech_005884 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005884 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005884 --> ing19_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005884 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Biotin["Biotin"]
    CultureMech_005884 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_005884 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_005884 --> ing23_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_HCl -.-> CHEBI_30961
    ing24_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_005884 --> ing24_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing24_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing25_Riboflavin["Riboflavin"]
    CultureMech_005884 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_005884 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_005884 --> ing27_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_005884 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_005884 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_005884 --> more["...6 more"]

Ingredients (36)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.294118 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	DSMZ Medium 318 lists 0.300 g KH2PO4 in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.294118 g/L in the 1020 mL formulation.
2	NaCl Cl.Na 58.443 g/mol	0.598039 G_PER_L	CHEBI:26710 sodium chloride	DSMZ Medium 318 lists 0.600 g NaCl in the main recipe and 10 mL of a trace solution containing 1.000 g/L NaCl in 1000 mL water plus 10 mL vitamin solution, yielding 0.598039 g/L in the 1020 mL formulation.
3	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.098039 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	DSMZ Medium 318 lists 0.100 g MgCl2 x 6 H2O in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.098039 g/L in the 1020 mL formulation.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.079412 G_PER_L	CHEBI:86158 calcium chloride dihydrate	DSMZ Medium 318 lists 0.080 g CaCl2 x 2 H2O in the main recipe and 10 mL of a trace solution containing 0.100 g/L CaCl2 x 2 H2O in 1000 mL water plus 10 mL vitamin solution, yielding 0.079412 g/L in the 1020 mL formulation.
5	NH4Cl Cl.H4N 53.492 g/mol	0.980392 G_PER_L	CHEBI:31206 ammonium chloride	DSMZ Medium 318 lists 1.000 g NH4Cl in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.980392 g/L in the 1020 mL formulation.
6	Yeast extract	0.490196 G_PER_L	FOODON:03315426 yeast extract	DSMZ Medium 318 lists 0.500 g Yeast extract in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.490196 g/L in the 1020 mL formulation.
7	Trypticase	0.490196 G_PER_L	MICRO:0000175 (source link unavailable) Trypticase	DSMZ Medium 318 lists 0.500 g Trypticase in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.490196 g/L in the 1020 mL formulation.
8	Resazurin C12H7NO4 229.191 g/mol	0.000980 G_PER_L	CHEBI:8806 Resazurin	DSMZ Medium 318 lists 1.000 mg Resazurin in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.000980 g/L in the 1020 mL formulation.
9	KHCO3 CHO3.K 100.114 g/mol	4.000000 G_PER_L	CHEBI:81862 potassium hydrogencarbonate	DSMZ Medium 522 sets KHCO3 to 4.000000 g/L.
10	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.294118 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	DSMZ Medium 318 lists 0.300 g Cysteine-HCl x H2O in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.294118 g/L in the 1020 mL formulation.
11	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.294118 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	DSMZ Medium 318 lists 0.300 g Na2S x 9 H2O in 1000 mL water and adds 10 mL trace element solution plus 10 mL vitamin solution; after omitting methanol this yields 0.294118 g/L in the 1020 mL formulation.
12	Nitrilotriacetic acid (NTA) C6H9NO6 191.139 g/mol	0.125490 G_PER_L	CHEBI:44557 nitrilotriacetic acid	DSMZ Medium 318 adds 10 mL of a trace element solution containing 12.800 g/L Nitrilotriacetic acid to 1000 mL water plus 10 mL vitamin solution, yielding 0.125490 g/L in the 1020 mL formulation.
13	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.013235 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 1.350 g/L FeCl3 x 6 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.013235 g/L in the 1020 mL formulation.
14	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.000980 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.100 g/L MnCl2 x 4 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000980 g/L in the 1020 mL formulation.
15	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000235 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.024 g/L CoCl2 x 6 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000235 g/L in the 1020 mL formulation.
16	ZnCl2 2Cl.Zn 136.296 g/mol	0.000980 G_PER_L	CHEBI:49976 zinc dichloride	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.100 g/L ZnCl2 to 1000 mL water plus 10 mL vitamin solution, yielding 0.000980 g/L in the 1020 mL formulation.
17	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.000245 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.025 g/L CuCl2 x 2 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000245 g/L in the 1020 mL formulation.
18	H3BO3 H3BO3 61.833 g/mol	0.000098 G_PER_L	CHEBI:33118 boric acid	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.010 g/L H3BO3 to 1000 mL water plus 10 mL vitamin solution, yielding 0.000098 g/L in the 1020 mL formulation.
19	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000235 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.024 g/L Na2MoO4 x 2 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000235 g/L in the 1020 mL formulation.
20	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.001176 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.120 g/L NiCl2 x 6 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.001176 g/L in the 1020 mL formulation.
21	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.000255 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	DSMZ Medium 318 adds 10 mL of a trace element solution containing 0.026 g/L Na2SeO3 x 5 H2O to 1000 mL water plus 10 mL vitamin solution, yielding 0.000255 g/L in the 1020 mL formulation.
22	Biotin C10H16N2O3S 244.316 g/mol	0.000019608 G_PER_L	CHEBI:15956 Biotin	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 2.00 mg/L Biotin, yielding 0.000019608 g/L in the 1020 mL formulation.
23	Folic acid C19H19N7O6 441.404 g/mol	0.000019608 G_PER_L	CHEBI:27470 Folic acid	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 2.00 mg/L Folic acid, yielding 0.000019608 g/L in the 1020 mL formulation.
24	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000098 G_PER_L	CHEBI:30961 Pyridoxine hydrochloride	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 10.00 mg/L Pyridoxine-HCl, yielding 0.000098 g/L in the 1020 mL formulation.
25	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000049 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L Thiamine-HCl x 2 H2O, yielding 0.000049 g/L in the 1020 mL formulation.
26	Riboflavin C17H20N4O6 376.369 g/mol	0.000049 G_PER_L	CHEBI:17015 Riboflavin	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L Riboflavin, yielding 0.000049 g/L in the 1020 mL formulation.
27	Nicotinic acid C6H5NO2 123.111 g/mol	0.000049 G_PER_L	CHEBI:15940 Nicotinic acid	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L Nicotinic acid, yielding 0.000049 g/L in the 1020 mL formulation.
28	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000049 G_PER_L	CHEBI:31345 Calcium pantothenate	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L D-Ca-pantothenate, yielding 0.000049 g/L in the 1020 mL formulation.
29	Vitamin B12	0.000000980 G_PER_L	CHEBI:176843 Vitamin B12	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 0.10 mg/L Vitamin B12, yielding 0.000000980 g/L in the 1020 mL formulation.
30	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000049 G_PER_L	CHEBI:30753 p-Aminobenzoic acid	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L p-Aminobenzoic acid, yielding 0.000049 g/L in the 1020 mL formulation.
31	(DL)-alpha-Lipoic acid C8H14O2S2 206.332 g/mol	0.000049 G_PER_L	CHEBI:16494 (DL)-alpha-Lipoic acid	DSMZ Medium 318 adds 10 mL of the DSMZ Medium 141 vitamin solution to 1000 mL water plus 10 mL trace element solution; the vitamin stock contains 5.00 mg/L Lipoic acid, yielding 0.000049 g/L in the 1020 mL formulation.
32	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	DSMZ Medium 318 adjusts the trace element solution to pH 6.5 with KOH.
33	N2 N2 28.014 g/mol	80 PERCENT_V_V	CHEBI:17997 dinitrogen	DSMZ Medium 318 uses an 80% N2 and 20% CO2 gas phase.
34	CO2 CO2 44.009 g/mol	20 PERCENT_V_V	CHEBI:16526 carbon dioxide	DSMZ Medium 318 uses an 80% N2 and 20% CO2 gas phase.
35	Distilled water H2O 18.015 g/mol	980.392157 ML_PER_L	CHEBI:15377 water	DSMZ Medium 318 lists 1000 mL water before 10 mL trace and 10 mL vitamin stock additions, yielding 980.392157 mL/L in the 1020 mL formulation after omitting methanol.
36	Ethanol C2H6O 46.069 g/mol	1.0 G_PER_L	CHEBI:16236 ethanol	DSMZ Medium 522 replaces methanol with 1.0 g/L ethanol.

Preparation

Prepare DSMZ Medium 318 without methanol and with KHCO3 at 4.0 g/L.
Add ethanol to 1.0 g/L.
Prepare the medium under an 80% N2 and 20% CO2 gas phase.
Sterilize vitamins, cysteine-HCl, and sulfide separately.
Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 522
2026-03-13T06:19:37.053920Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005884
2026-03-17T19:45:53.747495+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CLOSTRIDIUM NEOPROPIONICUM medium" to "clostridium_neopropionicum_medium" for consistent matching
2026-08-06T08:58:32.409823+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_418_522_score30.py	RESOLVED_KOMODO_418_522_SCORE30	DSMZ Medium 522 defines CLOSTRIDIUM NEOPROPIONICUM MEDIUM as DSMZ Medium 318 with 4.0 g/L KHCO3 for pH 7.0 and 1.0 g/L ethanol replacing methanol; this record expands the DSMZ Medium 318 trace element stock and the DSMZ Medium 141 vitamin stock into final per-liter components.